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Home / Equine Research Bank / Respir Res / Neutrophil extracellular traps are downregulated by glucocor...
Respiratory research2017; 18(1); 207; doi: 10.1186/s12931-017-0689-4

Neutrophil extracellular traps are downregulated by glucocorticosteroids in lungs in an equine model of asthma.

Abstract: Severe neutrophilic asthma is poorly responsive to glucocorticosteroids (GC). Neutrophil extracellular traps (NETs) within the lungs have been associated with the severity of airway obstruction and inflammation in asthma, and were found to be unaffected by GC in vitro. As IL-17 is overexpressed in neutrophilic asthma and contributes to steroid insensitivity in different cell types, we hypothesized that NETs formation in asthmatic airways would be resistant to GC through an IL-17 mediated pathway. Six neutrophilic severe asthmatic horses and six healthy controls were studied while being treated with dexamethasone. Lung function, bronchoalveolar lavage fluid (BALF) cytology and NETs formation, as well as the expression of CD11b and CD13 by blood and airway neutrophils were evaluated. The expression of IL-17 and its role in NETs formation were also studied. Airway neutrophils from asthmatic horses, as opposed to blood neutrophils, enhanced NETs formation, which was then decreased by GC. GC also tended to decrease the expression of CD11b in blood neutrophils, but not in airway neutrophils. IL-17 mRNA was increased in BALF cells of asthmatic horses and was unaffected by GC. However, both GC and IL-17 inhibited NETs formation in vitro. GC decreased NETs formation in vitro and also in vivo in the lungs of asthmatic horses. However, airway neutrophil activation during asthmatic inflammation was otherwise relatively insensitive to GC. The contribution of IL-17 to these responses requires further study.
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Publication Date: 2017-12-12 PubMed ID: 29233147PubMed Central: PMC5727947DOI: 10.1186/s12931-017-0689-4Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the effect of glucocorticosteroids (a kind of drug) on the structures called neutrophil extracellular traps in the lungs of horses that have a severe type of asthma. The findings suggest that glucocorticosteroids lessen the formation of these structures in the lungs but do not necessarily influence the behaviour of specific immune cells in the lungs.

Study Design and Purpose

  • The aim of this research was to investigate how neutrophil extracellular traps (NETs) behave in the presence of glucocorticosteroids (GC). NETs are DNA structures released by neutrophils, a type of white blood cell, to trap and kill pathogens. However, in a severe form of asthma often observed in horses, these NETs contribute to airway obstruction and inflammation.
  • The research sought to understand if GC treatment is effective in managing inflammatory parameters associated with neutrophilic severe asthma. Specifically, the study aimed to discover if GC could decrease the formation of NETs, especially considering that a protein called IL-17 believed to be overexpressed in neutrophilic asthma can contribute to steroid (like GC) insensitivity.

Research Methodology

  • The study involved twelve horses in total, six of which were severely asthmatic and the other six were healthy controls. All were treated with dexamethasone, a type of GC.
  • The researchers used various types of measurements to understand GC’s impact on NET generation and other inflammatory parameters. They investigated lung function, conducted bronchoalveolar lavage fluid (BALF) cytology (examination of cell types), measured NETs formation, and analysed the expression of certain proteins (CD11b and CD13) in blood and airway neutrophils.
  • The expression of IL-17 was also considered both in BALF cells and in NET formation.

Results

  • The results showed that when horses had asthmatic inflammation, their airway neutrophils significantly boosted the formation of NETs compared to their blood neutrophils.
  • This increased formation of NETs was successfully downregulated by GC treatment. However, the GC therapy did not notably impact the activation of airway neutrophils, indicating that certain aspects of airway neutrophil activation during asthma are insensitive to GC.
  • GC also lowered the expression of the protein CD11b in blood neutrophils, but not in airway neutrophils.
  • Another important finding was that the expression of IL-17 in BALF cells significantly increased in asthmatic horses. Surprisingly, GC did not appear to influence IL-17 expression. Still, both IL-17 and GC inhibited the formation of NETs in a controlled in vitro environment.

Conclusion

  • This research concluded that GC can induce a decrease in NETs formation both in vitro (lab test tubes) and in vivo (in the living organism, here, the lungs of asthmatic horses).
  • However, this study notes that the airway neutrophil activation, which is a significant aspect of asthma, was mostly unaffected by GC treatment. This implies that further research is required to comprehend the complex dynamics of neutrophil activity, NETs generation, and GC interaction, especially considering the role of IL-17.

Cite This Article

APA
Vargas A, Boivin R, Cano P, Murcia Y, Bazin I, Lavoie JP. (2017). Neutrophil extracellular traps are downregulated by glucocorticosteroids in lungs in an equine model of asthma. Respir Res, 18(1), 207. https://doi.org/10.1186/s12931-017-0689-4

Publication

Respiratory research
ISSN: 1465-993X
NlmUniqueID: 101090633
Country: England
Language: English
Volume: 18
Issue: 1
Pages: 207
PII: 207

Researcher Affiliations

Vargas, Amandine
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, 3200, rue Sicotte, Saint-Hyacinthe, Q, J2S 2M2, Canada.
Boivin, Roxane
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, 3200, rue Sicotte, Saint-Hyacinthe, Q, J2S 2M2, Canada.
Cano, Patricia
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, 3200, rue Sicotte, Saint-Hyacinthe, Q, J2S 2M2, Canada.
Murcia, Yoana
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, 3200, rue Sicotte, Saint-Hyacinthe, Q, J2S 2M2, Canada.
Bazin, Isabelle
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, 3200, rue Sicotte, Saint-Hyacinthe, Q, J2S 2M2, Canada.
Lavoie, Jean-Pierre
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, 3200, rue Sicotte, Saint-Hyacinthe, Q, J2S 2M2, Canada. jean-pierre.lavoie@umontreal.ca.

MeSH Terms

  • Animals
  • Asthma / drug therapy
  • Asthma / metabolism
  • Asthma / pathology
  • Disease Models, Animal
  • Down-Regulation / drug effects
  • Down-Regulation / physiology
  • Extracellular Traps / drug effects
  • Extracellular Traps / metabolism
  • Female
  • Glucocorticoids / pharmacology
  • Glucocorticoids / therapeutic use
  • Horses
  • Lung / drug effects
  • Lung / metabolism
  • Lung / pathology
  • Male
  • Neutrophils / drug effects
  • Neutrophils / metabolism
  • Neutrophils / pathology

Conflict of Interest Statement

ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

This article includes 49 references
  1. Bentley AM, Hamid Q, Robinson DS, Schotman E, Meng Q, Assoufi B, Kay AB, Durham SR. Prednisolone treatment in asthma. Reduction in the numbers of eosinophils, T cells, tryptase-only positive mast cells, and modulation of IL-4, IL-5, and interferon-gamma cytokine gene expression within the bronchial mucosa.. Am J Respir Crit Care Med 1996 Feb;153(2):551-6.
    doi: 10.1164/ajrccm.153.2.8564096pubmed: 8564096google scholar: lookup
  2. Hauber HP, Gotfried M, Newman K, Danda R, Servi RJ, Christodoulopoulos P, Hamid Q. Effect of HFA-flunisolide on peripheral lung inflammation in asthma.. J Allergy Clin Immunol 2003 Jul;112(1):58-63.
    doi: 10.1067/mai.2003.1612pubmed: 12847480google scholar: lookup
  3. Saffar AS, Ashdown H, Gounni AS. The molecular mechanisms of glucocorticoids-mediated neutrophil survival.. Curr Drug Targets 2011 Apr;12(4):556-62.
    doi: 10.2174/138945011794751555pmc: PMC3267167pubmed: 21504070google scholar: lookup
  4. Hirsch G, Lavoie-Lamoureux A, Beauchamp G, Lavoie JP. Neutrophils are not less sensitive than other blood leukocytes to the genomic effects of glucocorticoids.. PLoS One 2012;7(9):e44606.
    doi: 10.1371/journal.pone.0044606pmc: PMC3440353pubmed: 22984532google scholar: lookup
  5. Liu FC, Chuang YH, Tsai YF, Yu HP. Role of neutrophil extracellular traps following injury.. Shock 2014 Jun;41(6):491-8.
    doi: 10.1097/SHK.0000000000000146pubmed: 24837201google scholar: lookup
  6. McIlroy DJ, Jarnicki AG, Au GG, Lott N, Smith DW, Hansbro PM, Balogh ZJ. Mitochondrial DNA neutrophil extracellular traps are formed after trauma and subsequent surgery.. J Crit Care 2014 Dec;29(6):1133.e1-5.
    pubmed: 25128442doi: 10.1016/j.jcrc.2014.07.013google scholar: lookup
  7. Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss DS, Weinrauch Y, Zychlinsky A. Neutrophil extracellular traps kill bacteria.. Science 2004 Mar 5;303(5663):1532-5.
    doi: 10.1126/science.1092385pubmed: 15001782google scholar: lookup
  8. Garcia-Romo GS, Caielli S, Vega B, Connolly J, Allantaz F, Xu Z, Punaro M, Baisch J, Guiducci C, Coffman RL, Barrat FJ, Banchereau J, Pascual V. Netting neutrophils are major inducers of type I IFN production in pediatric systemic lupus erythematosus.. Sci Transl Med 2011 Mar 9;3(73):73ra20.
    doi: 10.1126/scitranslmed.3001201pmc: PMC3143837pubmed: 21389264google scholar: lookup
  9. Lande R, Ganguly D, Facchinetti V, Frasca L, Conrad C, Gregorio J, Meller S, Chamilos G, Sebasigari R, Riccieri V, Bassett R, Amuro H, Fukuhara S, Ito T, Liu YJ, Gilliet M. Neutrophils activate plasmacytoid dendritic cells by releasing self-DNA-peptide complexes in systemic lupus erythematosus.. Sci Transl Med 2011 Mar 9;3(73):73ra19.
    doi: 10.1126/scitranslmed.3001180pmc: PMC3399524pubmed: 21389263google scholar: lookup
  10. Neeli I, Dwivedi N, Khan S, Radic M. Regulation of extracellular chromatin release from neutrophils.. J Innate Immun 2009;1(3):194-201.
    doi: 10.1159/000206974pmc: PMC6951038pubmed: 20375577google scholar: lookup
  11. Lim MB, Kuiper JW, Katchky A, Goldberg H, Glogauer M. Rac2 is required for the formation of neutrophil extracellular traps.. J Leukoc Biol 2011 Oct;90(4):771-6.
    doi: 10.1189/jlb.1010549pubmed: 21712395google scholar: lookup
  12. Oehmcke S, Mörgelin M, Herwald H. Activation of the human contact system on neutrophil extracellular traps.. J Innate Immun 2009;1(3):225-30.
    doi: 10.1159/000203700pmc: PMC6951039pubmed: 20375580google scholar: lookup
  13. Fuchs TA, Abed U, Goosmann C, Hurwitz R, Schulze I, Wahn V, Weinrauch Y, Brinkmann V, Zychlinsky A. Novel cell death program leads to neutrophil extracellular traps.. J Cell Biol 2007 Jan 15;176(2):231-41.
    doi: 10.1083/jcb.200606027pmc: PMC2063942pubmed: 17210947google scholar: lookup
  14. Yuen J, Pluthero FG, Douda DN, Riedl M, Cherry A, Ulanova M, Kahr WH, Palaniyar N, Licht C. NETosing Neutrophils Activate Complement Both on Their Own NETs and Bacteria via Alternative and Non-alternative Pathways.. Front Immunol 2016;7:137.
    doi: 10.3389/fimmu.2016.00137pmc: PMC4831636pubmed: 27148258google scholar: lookup
  15. Chakravarti A, Rusu D, Flamand N, Borgeat P, Poubelle PE. Reprogramming of a subpopulation of human blood neutrophils by prolonged exposure to cytokines.. Lab Invest 2009 Oct;89(10):1084-99.
    doi: 10.1038/labinvest.2009.74pubmed: 19636293google scholar: lookup
  16. Wright TK, Gibson PG, Simpson JL, McDonald VM, Wood LG, Baines KJ. Neutrophil extracellular traps are associated with inflammation in chronic airway disease.. Respirology 2016 Apr;21(3):467-75.
    doi: 10.1111/resp.12730pubmed: 26804470google scholar: lookup
  17. Cortjens B, de Boer OJ, de Jong R, Antonis AF, Sabogal Piñeros YS, Lutter R, van Woensel JB, Bem RA. Neutrophil extracellular traps cause airway obstruction during respiratory syncytial virus disease.. J Pathol 2016 Feb;238(3):401-11.
    doi: 10.1002/path.4660pubmed: 26468056google scholar: lookup
  18. Grabcanovic-Musija F, Obermayer A, Stoiber W, Krautgartner WD, Steinbacher P, Winterberg N, Bathke AC, Klappacher M, Studnicka M. Neutrophil extracellular trap (NET) formation characterises stable and exacerbated COPD and correlates with airflow limitation.. Respir Res 2015 May 22;16(1):59.
    doi: 10.1186/s12931-015-0221-7pmc: PMC4455316pubmed: 25994149google scholar: lookup
  19. Lapponi MJ, Carestia A, Landoni VI, Rivadeneyra L, Etulain J, Negrotto S, Pozner RG, Schattner M. Regulation of neutrophil extracellular trap formation by anti-inflammatory drugs.. J Pharmacol Exp Ther 2013 Jun;345(3):430-7.
    doi: 10.1124/jpet.112.202879pubmed: 23536315google scholar: lookup
  20. Kolaczkowska E, Jenne CN, Surewaard BG, Thanabalasuriar A, Lee WY, Sanz MJ, Mowen K, Opdenakker G, Kubes P. Molecular mechanisms of NET formation and degradation revealed by intravital imaging in the liver vasculature.. Nat Commun 2015 Mar 26;6:6673.
    doi: 10.1038/ncomms7673pmc: PMC4389265pubmed: 25809117google scholar: lookup
  21. Lin AM, Rubin CJ, Khandpur R, Wang JY, Riblett M, Yalavarthi S, Villanueva EC, Shah P, Kaplan MJ, Bruce AT. Mast cells and neutrophils release IL-17 through extracellular trap formation in psoriasis.. J Immunol 2011 Jul 1;187(1):490-500.
    doi: 10.4049/jimmunol.1100123pmc: PMC3119764pubmed: 21606249google scholar: lookup
  22. Chesné J, Braza F, Mahay G, Brouard S, Aronica M, Magnan A. IL-17 in severe asthma. Where do we stand?. Am J Respir Crit Care Med 2014 Nov 15;190(10):1094-101.
    doi: 10.1164/rccm.201405-0859PPpubmed: 25162311google scholar: lookup
  23. Ricciardolo FLM, Sorbello V, Folino A, Gallo F, Massaglia GM, Favatà G, Conticello S, Vallese D, Gani F, Malerba M, Folkerts G, Rolla G, Profita M, Mauad T, Di Stefano A, Ciprandi G. Identification of IL-17F/frequent exacerbator endotype in asthma.. J Allergy Clin Immunol 2017 Aug;140(2):395-406.
    doi: 10.1016/j.jaci.2016.10.034pubmed: 27931975google scholar: lookup
  24. Murcia RY, Vargas A, Lavoie JP. The Interleukin-17 Induced Activation and Increased Survival of Equine Neutrophils Is Insensitive to Glucocorticoids.. PLoS One 2016;11(5):e0154755.
    doi: 10.1371/journal.pone.0154755pmc: PMC4854453pubmed: 27138006google scholar: lookup
  25. Leclere M, Lavoie-Lamoureux A, Lavoie JP. Heaves, an asthma-like disease of horses.. Respirology 2011 Oct;16(7):1027-46.
    pubmed: 21824219doi: 10.1111/j.1440-1843.2011.02033.xgoogle scholar: lookup
  26. Herszberg B, Ramos-Barbón D, Tamaoka M, Martin JG, Lavoie JP. Heaves, an asthma-like equine disease, involves airway smooth muscle remodeling.. J Allergy Clin Immunol 2006 Aug;118(2):382-8.
    doi: 10.1016/j.jaci.2006.03.044pubmed: 16890762google scholar: lookup
  27. Setlakwe EL, Lemos KR, Lavoie-Lamoureux A, Duguay JD, Lavoie JP. Airway collagen and elastic fiber content correlates with lung function in equine heaves.. Am J Physiol Lung Cell Mol Physiol 2014 Aug 1;307(3):L252-60.
    doi: 10.1152/ajplung.00019.2014pubmed: 24879055google scholar: lookup
  28. Bullone M, Moran K, Lavoie-Lamoureux A, Lavoie JP. PI3K and MAPKs regulate neutrophil migration toward the airways in heaves.. J Vet Intern Med 2013 Jan-Feb;27(1):164-70.
    doi: 10.1111/jvim.12008pubmed: 23194017google scholar: lookup
  29. Lavoie JP, Maghni K, Desnoyers M, Taha R, Martin JG, Hamid QA. Neutrophilic airway inflammation in horses with heaves is characterized by a Th2-type cytokine profile.. Am J Respir Crit Care Med 2001 Oct 15;164(8 Pt 1):1410-3.
    doi: 10.1164/ajrccm.164.8.2012091pubmed: 11704587google scholar: lookup
  30. Grishagin IV. Automatic cell counting with ImageJ.. Anal Biochem 2015 Mar 15;473:63-5.
    doi: 10.1016/j.ab.2014.12.007pubmed: 25542972google scholar: lookup
  31. da Cunha AA, Nuñez NK, de Souza RG, Moraes Vargas MH, Silveira JS, Antunes GL, Durante Lda S, Porto BN, Marczak ES, Jones MH, Pitrez PM. Recombinant human deoxyribonuclease therapy improves airway resistance and reduces DNA extracellular traps in a murine acute asthma model.. Exp Lung Res 2016;42(2):66-74.
    doi: 10.3109/01902148.2016.1143537pubmed: 27070484google scholar: lookup
  32. Porto BN, Stein RT. Neutrophil Extracellular Traps in Pulmonary Diseases: Too Much of a Good Thing?. Front Immunol 2016;7:311.
    doi: 10.3389/fimmu.2016.00311pmc: PMC4983612pubmed: 27574522google scholar: lookup
  33. Gibson PG, Simpson JL, Saltos N. Heterogeneity of airway inflammation in persistent asthma : evidence of neutrophilic inflammation and increased sputum interleukin-8.. Chest 2001 May;119(5):1329-36.
    doi: 10.1378/chest.119.5.1329pubmed: 11348936google scholar: lookup
  34. Wenzel SE, Szefler SJ, Leung DY, Sloan SI, Rex MD, Martin RJ. Bronchoscopic evaluation of severe asthma. Persistent inflammation associated with high dose glucocorticoids.. Am J Respir Crit Care Med 1997 Sep;156(3 Pt 1):737-43.
    doi: 10.1164/ajrccm.156.3.9610046pubmed: 9309987google scholar: lookup
  35. Kamath AV, Pavord ID, Ruparelia PR, Chilvers ER. Is the neutrophil the key effector cell in severe asthma?. Thorax 2005 Jul;60(7):529-30.
    doi: 10.1136/thx.2005.043182pmc: PMC1747453pubmed: 15994257google scholar: lookup
  36. Côté O, Clark ME, Viel L, Labbé G, Seah SY, Khan MA, Douda DN, Palaniyar N, Bienzle D. Secretoglobin 1A1 and 1A1A differentially regulate neutrophil reactive oxygen species production, phagocytosis and extracellular trap formation.. PLoS One 2014;9(4):e96217.
    doi: 10.1371/journal.pone.0096217pmc: PMC4002474pubmed: 24777050google scholar: lookup
  37. Dworski R, Simon HU, Hoskins A, Yousefi S. Eosinophil and neutrophil extracellular DNA traps in human allergic asthmatic airways.. J Allergy Clin Immunol 2011 May;127(5):1260-6.
    doi: 10.1016/j.jaci.2010.12.1103pmc: PMC3085562pubmed: 21315435google scholar: lookup
  38. Hamaguchi S, Hirose T, Matsumoto N, Akeda Y, Irisawa T, Seki M, Hosotsubo H, Yamamoto K, Tasaki O, Oishi K, Shimazu T, Tomono K. Neutrophil extracellular traps in bronchial aspirates: a quantitative analysis.. Eur Respir J 2014 Jun;43(6):1709-18.
    doi: 10.1183/09031936.00139813pubmed: 24603817google scholar: lookup
  39. Fiddler CA, Parfrey H, Cowburn AS, Luo D, Nash GB, Murphy G, Chilvers ER. The Aminopeptidase CD13 Induces Homotypic Aggregation in Neutrophils and Impairs Collagen Invasion.. PLoS One 2016;11(7):e0160108.
    doi: 10.1371/journal.pone.0160108pmc: PMC4965216pubmed: 27467268google scholar: lookup
  40. Mann BS, Chung KF. Blood neutrophil activation markers in severe asthma: lack of inhibition by prednisolone therapy.. Respir Res 2006 Apr 6;7(1):59.
    doi: 10.1186/1465-9921-7-59pmc: PMC1458332pubmed: 16600024google scholar: lookup
  41. Hennersdorf F, Florian S, Jakob A, Baumgärtner K, Sonneck K, Nordheim A, Biedermann T, Valent P, Bühring HJ. Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation.. Cell Res 2005 May;15(5):325-35.
    doi: 10.1038/sj.cr.7290301pubmed: 15916720google scholar: lookup
  42. Bartko J, Stiebellehner L, Derhaschnig U, Schoergenhofer C, Schwameis M, Prosch H, Jilma B. Dissociation between systemic and pulmonary anti-inflammatory effects of dexamethasone in humans.. Br J Clin Pharmacol 2016 May;81(5):865-77.
    doi: 10.1111/bcp.12857pmc: PMC4834593pubmed: 26647918google scholar: lookup
  43. McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK. TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.. J Immunol 2008 Sep 15;181(6):4089-97.
    doi: 10.4049/jimmunol.181.6.4089pmc: PMC3638757pubmed: 18768865google scholar: lookup
  44. Al-Ramli W, Préfontaine D, Chouiali F, Martin JG, Olivenstein R, Lemière C, Hamid Q. T(H)17-associated cytokines (IL-17A and IL-17F) in severe asthma.. J Allergy Clin Immunol 2009 May;123(5):1185-7.
    doi: 10.1016/j.jaci.2009.02.024pubmed: 19361847google scholar: lookup
  45. Bullens DM, Truyen E, Coteur L, Dilissen E, Hellings PW, Dupont LJ, Ceuppens JL. IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx?. Respir Res 2006 Nov 3;7(1):135.
    doi: 10.1186/1465-9921-7-135pmc: PMC1636037pubmed: 17083726google scholar: lookup
  46. Chrysanthopoulou A, Mitroulis I, Apostolidou E, Arelaki S, Mikroulis D, Konstantinidis T, Sivridis E, Koffa M, Giatromanolaki A, Boumpas DT, Ritis K, Kambas K. Neutrophil extracellular traps promote differentiation and function of fibroblasts.. J Pathol 2014 Jul;233(3):294-307.
    doi: 10.1002/path.4359pubmed: 24740698google scholar: lookup
  47. Narasaraju T, Yang E, Samy RP, Ng HH, Poh WP, Liew AA, Phoon MC, van Rooijen N, Chow VT. Excessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis.. Am J Pathol 2011 Jul;179(1):199-210.
    doi: 10.1016/j.ajpath.2011.03.013pmc: PMC3123873pubmed: 21703402google scholar: lookup
  48. Cox G. Glucocorticoid treatment inhibits apoptosis in human neutrophils. Separation of survival and activation outcomes.. J Immunol 1995 May 1;154(9):4719-25.
    pubmed: 7722324
  49. Zhang X, Moilanen E, Kankaanranta H. Beclomethasone, budesonide and fluticasone propionate inhibit human neutrophil apoptosis.. Eur J Pharmacol 2001 Nov 23;431(3):365-71.
    doi: 10.1016/S0014-2999(01)01437-6pubmed: 11730731google scholar: lookup

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  1. Lv B, Huang S, Huang H, Niu N, Liu J. Endothelial Glycocalyx Injury in SARS-CoV-2 Infection: Molecular Mechanisms and Potential Targeted Therapy.. Mediators Inflamm 2023;2023:6685251.
    doi: 10.1155/2023/6685251pubmed: 37674786google scholar: lookup
  2. Kim JH, Na YS, Lee SI, Moon YY, Hwang BS, Baek AR, Kim WY, Lee BY, Seong GM, Baek MS. Corticosteroid outcome may be dependent of duration of use in severe COVID-19.. Korean J Intern Med 2023 May;38(3):382-392.
    doi: 10.3904/kjim.2022.201pubmed: 37038264google scholar: lookup
  3. Salinas C, Barriga K, Albornoz A, Alarcon P, Quiroga J, Uberti B, Sarmiento J, Henriquez C, Ehrenfeld P, Burgos RA, Moran G. Tamoxifen triggers the in vitro release of neutrophil extracellular traps in healthy horses.. Front Vet Sci 2022;9:1025249.
    doi: 10.3389/fvets.2022.1025249pubmed: 36686170google scholar: lookup
  4. Sim S, Choi Y, Park HS. Immunologic Basis of Type 2 Biologics for Severe Asthma.. Immune Netw 2022 Dec;22(6):e45.
    doi: 10.4110/in.2022.22.e45pubmed: 36627938google scholar: lookup
  5. Poto R, Shamji M, Marone G, Durham SR, Scadding GW, Varricchi G. Neutrophil Extracellular Traps in Asthma: Friends or Foes?. Cells 2022 Nov 7;11(21).
    doi: 10.3390/cells11213521pubmed: 36359917google scholar: lookup
  6. Sage SE, Nicholson P, Peters LM, Leeb T, Jagannathan V, Gerber V. Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells.. Front Immunol 2022;13:929922.
    doi: 10.3389/fimmu.2022.929922pubmed: 36105804google scholar: lookup
  7. Hoffmann ALC, Hauck SM, Deeg CA, Degroote RL. Pre-Activated Granulocytes from an Autoimmune Uveitis Model Show Divergent Pathway Activation Profiles upon IL8 Stimulation In Vitro.. Int J Mol Sci 2022 Aug 23;23(17).
    doi: 10.3390/ijms23179555pubmed: 36076947google scholar: lookup
  8. Wang J, Li Q, Qiu Y, Lu H. COVID-19: imbalanced cell-mediated immune response drives to immunopathology.. Emerg Microbes Infect 2022 Dec;11(1):2393-2404.
    doi: 10.1080/22221751.2022.2122579pubmed: 36069182google scholar: lookup
  9. Janssen P, Tosi I, Hego A, Maréchal P, Marichal T, Radermecker C. Neutrophil Extracellular Traps Are Found in Bronchoalveolar Lavage Fluids of Horses With Severe Asthma and Correlate With Asthma Severity.. Front Immunol 2022;13:921077.
    doi: 10.3389/fimmu.2022.921077pubmed: 35911691google scholar: lookup
  10. Mukherjee P, Roy S, Ghosh D, Nandi SK. Role of animal models in biomedical research: a review.. Lab Anim Res 2022 Jul 1;38(1):18.
    doi: 10.1186/s42826-022-00128-1pubmed: 35778730google scholar: lookup
  11. Ventura-Santana E, Ninan JR, Snyder CM, Okeke EB. Neutrophil Extracellular Traps, Sepsis and COVID-19 - A Tripod Stand.. Front Immunol 2022;13:902206.
    doi: 10.3389/fimmu.2022.902206pubmed: 35757734google scholar: lookup
  12. Block H, Rossaint J, Zarbock A. The Fatal Circle of NETs and NET-Associated DAMPs Contributing to Organ Dysfunction.. Cells 2022 Jun 14;11(12).
    doi: 10.3390/cells11121919pubmed: 35741047google scholar: lookup
  13. Morán G, Uberti B, Quiroga J. Role of Cellular Metabolism in the Formation of Neutrophil Extracellular Traps in Airway Diseases.. Front Immunol 2022;13:850416.
    doi: 10.3389/fimmu.2022.850416pubmed: 35493475google scholar: lookup
  14. Simões J, Batista M, Tilley P. The Immune Mechanisms of Severe Equine Asthma-Current Understanding and What Is Missing.. Animals (Basel) 2022 Mar 16;12(6).
    doi: 10.3390/ani12060744pubmed: 35327141google scholar: lookup
  15. Rebelatto CLK, Senegaglia AC, Franck CL, Daga DR, Shigunov P, Stimamiglio MA, Marsaro DB, Schaidt B, Micosky A, de Azambuja AP, Leitão CA, Petterle RR, Jamur VR, Vaz IM, Mallmann AP, Carraro Junior H, Ditzel E, Brofman PRS, Correa A. Safety and long-term improvement of mesenchymal stromal cell infusion in critically COVID-19 patients: a randomized clinical trial.. Stem Cell Res Ther 2022 Mar 21;13(1):122.
    doi: 10.1186/s13287-022-02796-1pubmed: 35313959google scholar: lookup
  16. Theuerkauf K, Obach-Schröck C, Staszyk C, Moritz A, Roscher KA. Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.. J Vet Diagn Invest 2022 May;34(3):448-457.
    doi: 10.1177/10406387221077969pubmed: 35168432google scholar: lookup
  17. Umbrello M, Formenti P, Nespoli S, Pisano E, Bonino C, Muttini S. Effect of Different Corticosteroid Regimens on the Outcome of Severe COVID-19-Related Acute Respiratory Failure. A Retrospective Analysis.. J Clin Med 2021 Oct 21;10(21).
    doi: 10.3390/jcm10214847pubmed: 34768369google scholar: lookup
  18. Clemente-Moragón A, Martínez-Milla J, Oliver E, Santos A, Flandes J, Fernández I, Rodríguez-González L, Serrano Del Castillo C, Ioan AM, López-Álvarez M, Gómez-Talavera S, Galán-Arriola C, Fuster V, Pérez-Calvo C, Ibáñez B. Metoprolol in Critically Ill Patients With COVID-19.. J Am Coll Cardiol 2021 Sep 7;78(10):1001-1011.
    doi: 10.1016/j.jacc.2021.07.003pubmed: 34474731google scholar: lookup
  19. Block H, Zarbock A. A Fragile Balance: Does Neutrophil Extracellular Trap Formation Drive Pulmonary Disease Progression?. Cells 2021 Jul 29;10(8).
    doi: 10.3390/cells10081932pubmed: 34440701google scholar: lookup
  20. Steffensen N, Imker R, Lassnig S, Fulde M, Rieder JC, de Buhr N. Methylprednisolone Induces Extracellular Trap Formation and Enhances Bactericidal Effect of Canine Neutrophils.. Int J Mol Sci 2021 Jul 20;22(14).
    doi: 10.3390/ijms22147734pubmed: 34299355google scholar: lookup
  21. Bjørnkjær-Nielsen KA, Bjørnvad CR. Corticosteroid treatment for acute/acute-on-chronic experimental and naturally occurring pancreatitis in several species: a scoping review to inform possible use in dogs.. Acta Vet Scand 2021 Jul 13;63(1):28.
    doi: 10.1186/s13028-021-00592-0pubmed: 34256804google scholar: lookup
  22. Mainguy-Seers S, Lavoie JP. Glucocorticoid treatment in horses with asthma: A narrative review.. J Vet Intern Med 2021 Jul;35(4):2045-2057.
    doi: 10.1111/jvim.16189pubmed: 34085342google scholar: lookup
  23. Millares-Ramirez EM, Lavoie JP. Bronchial angiogenesis in horses with severe asthma and its response to corticosteroids.. J Vet Intern Med 2021 Jul;35(4):2026-2034.
    doi: 10.1111/jvim.16159pubmed: 34048095google scholar: lookup
  24. Ackermann M, Anders HJ, Bilyy R, Bowlin GL, Daniel C, De Lorenzo R, Egeblad M, Henneck T, Hidalgo A, Hoffmann M, Hohberger B, Kanthi Y, Kaplan MJ, Knight JS, Knopf J, Kolaczkowska E, Kubes P, Leppkes M, Mahajan A, Manfredi AA, Maueröder C, Maugeri N, Mitroulis I, Muñoz LE, Narasaraju T, Naschberger E, Neeli I, Ng LG, Radic MZ, Ritis K, Rovere-Querini P, Schapher M, Schauer C, Simon HU, Singh J, Skendros P, Stark K, Stürzl M, van der Vlag J, Vandenabeele P, Vitkov L, von Köckritz-Blickwede M, Yanginlar C, Yousefi S, Zarbock A, Schett G, Herrmann M. Patients with COVID-19: in the dark-NETs of neutrophils.. Cell Death Differ 2021 Nov;28(11):3125-3139.
    doi: 10.1038/s41418-021-00805-zpubmed: 34031543google scholar: lookup
  25. Zuo Y, Kanthi Y, Knight JS, Kim AHJ. The interplay between neutrophils, complement, and microthrombi in COVID-19.. Best Pract Res Clin Rheumatol 2021 Mar;35(1):101661.
    doi: 10.1016/j.berh.2021.101661pubmed: 33526325google scholar: lookup
  26. Trivedi A, Khan MA, Bade G, Talwar A. Orchestration of Neutrophil Extracellular Traps (Nets), a Unique Innate Immune Function during Chronic Obstructive Pulmonary Disease (COPD) Development.. Biomedicines 2021 Jan 8;9(1).
    doi: 10.3390/biomedicines9010053pubmed: 33435568google scholar: lookup
  27. Flanders AJ, Ossiboff RJ, Wellehan JFX Jr, Alexander AB, Fredholm DVE, Desiderio TM, Stacy NI. Presumptive heterophil extracellular traps recognized cytologically in nine reptile patients with inflammatory conditions.. Vet Q 2021 Dec;41(1):89-96.
    doi: 10.1080/01652176.2021.1873453pubmed: 33416037google scholar: lookup
  28. Vitkov L, Minnich B, Knopf J, Schauer C, Hannig M, Herrmann M. NETs Are Double-Edged Swords with the Potential to Aggravate or Resolve Periodontal Inflammation.. Cells 2020 Dec 5;9(12).
    doi: 10.3390/cells9122614pubmed: 33291407google scholar: lookup
  29. Wan T, Zhang Y, Yuan K, Min J, Mou Y, Jin X. Acetylsalicylic Acid Promotes Corneal Epithelium Migration by Regulating Neutrophil Extracellular Traps in Alkali Burn.. Front Immunol 2020;11:551057.
    doi: 10.3389/fimmu.2020.551057pubmed: 33178183google scholar: lookup
  30. Davis KU, Sheats MK. The Role of Neutrophils in the Pathophysiology of Asthma in Humans and Horses.. Inflammation 2021 Apr;44(2):450-465.
    doi: 10.1007/s10753-020-01362-2pubmed: 33150539google scholar: lookup
  31. Arabi YM, Chrousos GP, Meduri GU. The ten reasons why corticosteroid therapy reduces mortality in severe COVID-19.. Intensive Care Med 2020 Nov;46(11):2067-2070.
    doi: 10.1007/s00134-020-06223-ypubmed: 33026460google scholar: lookup
  32. Thierry AR, Roch B. Neutrophil Extracellular Traps and By-Products Play a Key Role in COVID-19: Pathogenesis, Risk Factors, and Therapy.. J Clin Med 2020 Sep 11;9(9).
    doi: 10.3390/jcm9092942pubmed: 32933031google scholar: lookup
  33. Couetil L, Cardwell JM, Leguillette R, Mazan M, Richard E, Bienzle D, Bullone M, Gerber V, Ivester K, Lavoie JP, Martin J, Moran G, Niedźwiedź A, Pusterla N, Swiderski C. Equine Asthma: Current Understanding and Future Directions.. Front Vet Sci 2020;7:450.
    doi: 10.3389/fvets.2020.00450pubmed: 32903600google scholar: lookup
  34. Schönrich G, Raftery MJ, Samstag Y. Devilishly radical NETwork in COVID-19: Oxidative stress, neutrophil extracellular traps (NETs), and T cell suppression.. Adv Biol Regul 2020 Aug;77:100741.
    doi: 10.1016/j.jbior.2020.100741pubmed: 32773102google scholar: lookup
  35. Dominguez EC, Heires AJ, Pavlik J, Larsen TD, Guardado S, Sisson JH, Baack ML, Romberger DJ, Nordgren TM. A High Docosahexaenoic Acid Diet Alters the Lung Inflammatory Response to Acute Dust Exposure.. Nutrients 2020 Aug 4;12(8).
    doi: 10.3390/nለ2334pubmed: 32759853google scholar: lookup
  36. Kuczia P, Zuk J, Iwaniec T, Soja J, Dropinski J, Malesa-Wlodzik M, Zareba L, Bazan JG, Undas A, Bazan-Socha S. Citrullinated histone H3, a marker of extracellular trap formation, is increased in blood of stable asthma patients.. Clin Transl Allergy 2020;10:31.
    doi: 10.1186/s13601-020-00337-8pubmed: 32685129google scholar: lookup
  37. Neumann A, Brogden G, von Köckritz-Blickwede M. Extracellular Traps: An Ancient Weapon of Multiple Kingdoms.. Biology (Basel) 2020 Feb 18;9(2).
    doi: 10.3390/biology9020034pubmed: 32085405google scholar: lookup
  38. Degroote RL, Weigand M, Hauck SM, Deeg CA. IL8 and PMA Trigger the Regulation of Different Biological Processes in Granulocyte Activation.. Front Immunol 2019;10:3064.
    doi: 10.3389/fimmu.2019.03064pubmed: 32010136google scholar: lookup
  39. Ravindran M, Khan MA, Palaniyar N. Neutrophil Extracellular Trap Formation: Physiology, Pathology, and Pharmacology.. Biomolecules 2019 Aug 14;9(8).
    doi: 10.3390/biom9080365pubmed: 31416173google scholar: lookup
  40. Sheats MK. A Comparative Review of Equine SIRS, Sepsis, and Neutrophils.. Front Vet Sci 2019;6:69.
    doi: 10.3389/fvets.2019.00069pubmed: 30931316google scholar: lookup
  41. Ruggeri P, Caramori G. Functional Role of Inflammasome Activation in a Subset of Obese Nonsmoking Patients with Severe Asthma.. Am J Respir Crit Care Med 2019 May 1;199(9):1045-1047.
    doi: 10.1164/rccm.201903-0667EDpubmed: 30908928google scholar: lookup
  42. Bond S, Léguillette R, Richard EA, Couetil L, Lavoie JP, Martin JG, Pirie RS. Equine asthma: Integrative biologic relevance of a recently proposed nomenclature.. J Vet Intern Med 2018 Nov;32(6):2088-2098.
    doi: 10.1111/jvim.15302pubmed: 30294851google scholar: lookup
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