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Veterinary sciences2021; 8(5); 79; doi: 10.3390/vetsci8050079

New Sperm Morphology Analysis in Equids: Trumorph® Vs Eosin-Nigrosin Stain.

Abstract: The evaluation of the male fertility potential is based on the analysis of the basic spermatic characteristics of concentration, motility and morphology. Thus, the study of sperm morphology is a fundamental element in the seminal analysis, but its real meaning has been biased by the techniques used for its evaluation. These techniques involve dehydration phases and subsequent staining, which involves the production of artifacts. The aim of the study is to compare two methods for equid semen morphology evaluation, Trumorph using living sperm vs. eosin-nigrosine stain. A total of 49 ejaculates from stallions and donkeys were used. After semen collection and dilution, an aliquot was placed on the slide and introduced in the Trumorph device. Then observation was made with a 40x objective and negative phase-contrast microscope. Another aliquot was stained using eosin-nigrosine stain and viewed using 100× magnification. Well-formed sperm were observed, and different abnormalities were identified using Trumorph. The use of eosin-nigrosin staining method and Trumorph led to the same results and both techniques can be used for stallion and donkey sperm morphological analysis. However, considering the fact that Trumorph uses living sperm helps prevent sperm cell alteration during sample preparation. Therefore, Trumorph can be a good alternative to the conventional staining method, which provides a quick test on live sperm.
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Publication Date: 2021-05-06 PubMed ID: 34066550PubMed Central: PMC8148583DOI: 10.3390/vetsci8050079Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study aims to compare two methods used in assessing horse sperm morphology: Trumorph technique that uses live sperm and the eosin-nigrosine stain method. The Trumorph proved to be a viable alternative as both methods presented similar results, but Trumorph has the advantage of preventing sperm cell alteration during sample preparation.

Comparing Two Sperm Morphology Evaluation Methods

  • The researchers are comparing two techniques used in the analysis of sperm morphology as part of evaluating male fertility. The methods compared were Trumorph, which uses living sperm, and the eosin-nigrosine stain technique.
  • Understanding sperm morphology is a key part of seminal analysis and fertility evaluation, but the meaning and accuracy of such analyses can be compromised by the techniques used.
  • Traditional methods of sperm morphology evaluation often involve the dehydration and staining of sperm, which can produce artifacts which may distort the results.

Trumorph vs Eosin-Nigrosin Staining Technique

  • The researchers used a total of 49 ejaculates from stallions and donkeys. They first collected and diluted the semen, and placed an aliquot on a slide and introduced it in the Trumorph device.
  • They observed the sperm using a 40x objective and negative phase-contrast microscope. They noted well-formed sperm and various sperm abnormalities.
  • They performed the same process with another aliquot using the eosin-nigrosine stain method, but in this case, they viewed the sample using 100x magnification. The two techniques were compared based on their results.

Research Findings and Conclusion

  • The researchers found that both the eosin-nigrosine staining method and Trumorph led to the same results, suggesting that either technique can be used for stallion and donkey sperm morphological analysis.
  • However, given that Trumorph uses living sperm, it avoids the possibility of sperm cell alteration during sample preparation. This makes it potentially more reliable.
  • Consequently, the researchers concluded that Trumorph is a good alternative to the conventional staining method as it allows for quick testing on live sperm without the risk of altering sperm cells.

Cite This Article

APA
Gacem S, Catalán J, Yánez-Ortiz I, Soler C, Miró J. (2021). New Sperm Morphology Analysis in Equids: Trumorph® Vs Eosin-Nigrosin Stain. Vet Sci, 8(5), 79. https://doi.org/10.3390/vetsci8050079

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 8
Issue: 5
PII: 79

Researcher Affiliations

Gacem, Sabrina
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain.
Catalán, Jaime
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain.
Yánez-Ortiz, Iván
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain.
Soler, Carles
  • Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100, Burjassot, Valencia, Spain. Proiser R+D, Scientific Park, University of Valencia, C/ Catedràtic Agustín Escardino, 9, Building 3 (CUE), Floor 1, 46980 Paterna, Spain.
Miró, Jordi
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Veterinary Faculty, Autonomous University of Barcelona, 08193 Bellaterra, Spain.

Conflict of Interest Statement

The authors declare no conflict of interest. One author (C.S.) is employee for the company that manufacture the CASA-Mot system and the Trumorph machine used. In any case, it does not imply any conflict of interest, because no publicity (direct or indirect) is done in the manuscript. These components were just used in the same way we could use from other brands and the elements studied are not related with the brand but with physic and biological topics.

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Citations

This article has been cited 8 times.
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