Notes from the Field: Multistate Outbreak of Eastern Equine Encephalitis Virus – United States, 2019.

1. Calisher CH. Medically important arboviruses of the United States and Canada.. Clin Microbiol Rev 1994 Jan;7(1):89-116.
   doi: 10.1128/CMR.7.1.89pmc: PMC358307pubmed: 8118792google scholar: lookup
2. Silverman MA, Misasi J, Smole S, Feldman HA, Cohen AB, Santagata S, McManus M, Ahmed AA. Eastern equine encephalitis in children, Massachusetts and New Hampshire,USA, 1970-2010.. Emerg Infect Dis 2013 Feb;19(2):194-201; quiz 352.
   doi: 10.3201/eid1902.120039pmc: PMC3559032pubmed: 23343480google scholar: lookup
3. Lindsey NP, Staples JE, Fischer M. Eastern Equine Encephalitis Virus in the United States, 2003-2016.. Am J Trop Med Hyg 2018 May;98(5):1472-1477.
   doi: 10.4269/ajtmh.17-0927pmc: PMC5953388pubmed: 29557336google scholar: lookup
4. CDC. Eastern equine encephalitis virus: statistics & maps. Fort Collins, CO: US Department of Health and Human Services, CDC; 2019. https://www.cdc.gov/easternequineencephalitis/tech/epi.html
5. Getting VA. Equine encephalomyelitis in Massachusetts: an analysis of the 1938 outbreak, a follow-up of cases and a report of a mosquito survey.. NEJM 1941;224:99–1006.
   doi: 10.3201/eid1902.120039pubmed: 0google scholar: lookup
6. Feemster RF. Outbreak of Encephalitis in Man Due to the Eastern Virus of Equine Encephalomyelitis.. Am J Public Health Nations Health 1938 Dec;28(12):1403-10.
   doi: 10.2105/AJPH.28.12.1403pmc: PMC1527806pubmed: 18014957google scholar: lookup
7. Goldfield M, Sussman O. The 1959 outbreak of Eastern encephalitis in New Jersey. I. Introduction and description of outbreak.. Am J Epidemiol 1968 Jan;87(1):1-10.
   doi: 10.1093/oxfordjournals.aje.a120789pubmed: 5637871google scholar: lookup

Citations

1. Langsjoen RM, Key A, Shariatzadeh N, Jackson CR, Mahmood F, Arkun K, Alexandrescu S, Solomon IH, Piantadosi A. Eastern Equine Encephalitis Virus Diversity in Massachusetts Patients, 1938-2020. Am J Trop Med Hyg 2023 Aug 2;109(2):387-396.
   doi: 10.4269/ajtmh.23-0047pubmed: 37339758google scholar: lookup
2. Hill V, Koch RT, Bialosuknia SM, Ngo K, Zink SD, Koetzner CA, Maffei JG, Dupuis AP, Backenson PB, Oliver J, Bransfield AB, Misencik MJ, Petruff TA, Shepard JJ, Warren JL, Gill MS, Baele G, Vogels CBF, Gallagher G, Burns P, Hentoff A, Smole S, Brown C, Osborne M, Kramer LD, Armstrong PM, Ciota AT, Grubaugh ND. Dynamics of eastern equine encephalitis virus during the 2019 outbreak in the Northeast United States. Curr Biol 2023 Jun 19;33(12):2515-2527.e6.
   doi: 10.1016/j.cub.2023.05.047pubmed: 37295427google scholar: lookup
3. Ladzinski AT, Tai A, Rumschlag MT, Smith CS, Mehta A, Boapimp P, Edewaard EJ, Douce RW, Morgan LF, Wang MS, Fisher-Hubbard AO, Cummings MJ, Jagger BW. Clinical Characteristics of the 2019 Eastern Equine Encephalitis Outbreak in Michigan. Open Forum Infect Dis 2023 May;10(5):ofad206.
   doi: 10.1093/ofid/ofad206pubmed: 37180595google scholar: lookup
4. Zang C, Wang X, Cheng P, Liu L, Guo X, Wang H, Lou Z, Lei J, Wang W, Wang Y, Gong M, Liu H. Evaluation of the evolutionary genetics and population structure of Culex pipiens pallens in Shandong province, China based on knockdown resistance (kdr) mutations and the mtDNA-COI gene. BMC Genomics 2023 Mar 24;24(1):145.
   doi: 10.1186/s12864-023-09243-2pubmed: 36964519google scholar: lookup
5. Williamson LE, Bandyopadhyay A, Bailey K, Sirohi D, Klose T, Julander JG, Kuhn RJ, Crowe JE Jr. Structural constraints link differences in neutralization potency of human anti-Eastern equine encephalitis virus monoclonal antibodies. Proc Natl Acad Sci U S A 2023 Mar 28;120(13):e2213690120.
   doi: 10.1073/pnas.2213690120pubmed: 36961925google scholar: lookup
6. Hill V, Koch RT, Bialosuknia SM, Ngo K, Zink SD, Koetzner CA, Maffei JG, Dupuis AP, Backenson PB, Oliver J, Bransfield AB, Misencik MJ, Petruff TA, Shepard JJ, Warren JL, Gill MS, Baele G, Vogels CBF, Gallagher G, Burns P, Hentoff A, Smole S, Brown C, Osborne M, Kramer LD, Armstrong PM, Ciota AT, Grubaugh ND. Dynamics of Eastern equine encephalitis virus during the 2019 outbreak in the Northeast United States. medRxiv 2023 Mar 6;.
   doi: 10.1101/2023.03.06.23286851pubmed: 36945576google scholar: lookup
7. Ogorek TJ, Golden JE. Advances in the Development of Small Molecule Antivirals against Equine Encephalitic Viruses. Viruses 2023 Feb 1;15(2).
   doi: 10.3390/v15020413pubmed: 36851628google scholar: lookup
8. Chen CL, Klose T, Sun C, Kim AS, Buda G, Rossmann MG, Diamond MS, Klimstra WB, Kuhn RJ. Cryo-EM structures of alphavirus conformational intermediates in low pH-triggered prefusion states. Proc Natl Acad Sci U S A 2022 Jul 26;119(30):e2114119119.
   doi: 10.1073/pnas.2114119119pubmed: 35867819google scholar: lookup
9. Ryan MC, Kim E, Cao X, Reichard W, Ogorek TJ, Das P, Jonsson CB, Baudry J, Chung D, Golden JE. Piperazinobenzodiazepinones: New Encephalitic Alphavirus Inhibitors via Ring Expansion of 2-Dichloromethylquinazolinones. ACS Med Chem Lett 2022 Apr 14;13(4):546-553.
   doi: 10.1021/acsmedchemlett.1c00539pubmed: 35450382google scholar: lookup
10. Tang X, Sedda L, Brown HE. Predicting eastern equine encephalitis spread in North America: An ecological study. Curr Res Parasitol Vector Borne Dis 2021;1:100064.
    doi: 10.1016/j.crpvbd.2021.100064pubmed: 35284888google scholar: lookup
11. Montalvo M, Ayoub D, McGary M, Byrd K, Mahmoud L, Mermel L, Thompson B, Wendell L. Eastern Equine Encephalitis: Case Series in Southern New England and Review of the Literature. Neurol Clin Pract 2021 Oct;11(5):e714-e721.
    doi: 10.1212/CPJ.0000000000001079pubmed: 34840888google scholar: lookup
12. Stobierski MG, Signs K, Dinh E, Cooley TM, Melotti J, Schalow M, Patterson JS, Bolin SR, Walker ED. Eastern Equine Encephalomyelitis in Michigan: Historical Review of Equine, Human, and Wildlife Involvement, Epidemiology, Vector Associations, and Factors Contributing to Endemicity. J Med Entomol 2022 Jan 12;59(1):27-40.
    doi: 10.1093/jme/tjab153pubmed: 34734638google scholar: lookup
13. Armstrong PM, Andreadis TG. Ecology and Epidemiology of Eastern Equine Encephalitis Virus in the Northeastern United States: An Historical Perspective. J Med Entomol 2022 Jan 12;59(1):1-13.
    doi: 10.1093/jme/tjab077pubmed: 34734628google scholar: lookup
14. Gorris ME, Bartlow AW, Temple SD, Romero-Alvarez D, Shutt DP, Fair JM, Kaufeld KA, Del Valle SY, Manore CA. Updated distribution maps of predominant Culex mosquitoes across the Americas. Parasit Vectors 2021 Oct 23;14(1):547.
    doi: 10.1186/s13071-021-05051-3pubmed: 34688314google scholar: lookup
15. Calvert AE, Bennett SL, Hunt AR, Fong RH, Doranz BJ, Roehrig JT, Blair CD. Exposing cryptic epitopes on the Venezuelan equine encephalitis virus E1 glycoprotein prior to treatment with alphavirus cross-reactive monoclonal antibody allows blockage of replication early in infection. Virology 2022 Jan 2;565:13-21.
    doi: 10.1016/j.virol.2021.09.007pubmed: 34626907google scholar: lookup
16. Hasan SS, Dey D, Singh S, Martin M. The Structural Biology of Eastern Equine Encephalitis Virus, an Emerging Viral Threat. Pathogens 2021 Jul 31;10(8).
    doi: 10.3390/pathogens10080973pubmed: 34451437google scholar: lookup
17. Williamson LE, Reeder KM, Bailey K, Tran MH, Roy V, Fouch ME, Kose N, Trivette A, Nargi RS, Winkler ES, Kim AS, Gainza C, Rodriguez J, Armstrong E, Sutton RE, Reidy J, Carnahan RH, McDonald WH, Schoeder CT, Klimstra WB, Davidson E, Doranz BJ, Alter G, Meiler J, Schey KL, Julander JG, Diamond MS, Crowe JE Jr. Therapeutic alphavirus cross-reactive E1 human antibodies inhibit viral egress. Cell 2021 Aug 19;184(17):4430-4446.e22.
    doi: 10.1016/j.cell.2021.07.033pubmed: 34416147google scholar: lookup
18. Brown SC, Cormier J, Tuan J, Lier AJ, McGuone D, Armstrong PM, Kaddouh F, Parikh S, Landry ML, Gobeske KT. Four Human Cases of Eastern Equine Encephalitis in Connecticut, USA, during a Larger Regional Outbreak, 2019. Emerg Infect Dis 2021 Aug;27(8):2042-51.
    doi: 10.3201/eid2708.203730pubmed: 34289334google scholar: lookup
19. Hughes HR, Velez JO, Davis EH, Laven J, Gould CV, Panella AJ, Lambert AJ, Staples JE, Brault AC. Fatal Human Infection with Evidence of Intrahost Variation of Eastern Equine Encephalitis Virus, Alabama, USA, 2019. Emerg Infect Dis 2021 Jul;27(7):1886-1892.
    doi: 10.3201/eid2707.210315pubmed: 34152960google scholar: lookup
20. Millet N, Faiek S, Gurrieri D, Kals K, Adams W, Hamaty E, Trivedi M, Zeidwerg D. Deadly Neuroinvasive Mosquito-Borne Virus: A Case of Eastern Equine Encephalitis. Perm J 2021 May;25.
    doi: 10.7812/TPP/20.288pubmed: 33970094google scholar: lookup
21. Henning L, Endt K, Steigerwald R, Anderson M, Volkmann A. A Monovalent and Trivalent MVA-Based Vaccine Completely Protects Mice Against Lethal Venezuelan, Western, and Eastern Equine Encephalitis Virus Aerosol Challenge. Front Immunol 2020;11:598847.
    doi: 10.3389/fimmu.2020.598847pubmed: 33542715google scholar: lookup
22. Azar SR, Campos RK, Bergren NA, Camargos VN, Rossi SL. Epidemic Alphaviruses: Ecology, Emergence and Outbreaks. Microorganisms 2020 Aug 1;8(8).
    doi: 10.3390/microorganisms8081167pubmed: 32752150google scholar: lookup
23. Oliver J, Tan Y, Haight JD, Tober KJ, Gall WK, Zink SD, Kramer LD, Campbell SR, Howard JJ, Das SR, Sherwood JA. Spatial and temporal expansions of Eastern equine encephalitis virus and phylogenetic groups isolated from mosquitoes and mammalian cases in New York State from 2013 to 2019. Emerg Microbes Infect 2020 Dec;9(1):1638-1650.
    doi: 10.1080/22221751.2020.1774426pubmed: 32672516google scholar: lookup
24. Meshram CD, Shiliaev N, Frolova EI, Frolov I. Hypervariable Domain of nsP3 of Eastern Equine Encephalitis Virus Is a Critical Determinant of Viral Virulence. J Virol 2020 Aug 17;94(17).
    doi: 10.1128/JVI.00617-20pubmed: 32581106google scholar: lookup
25. Kading RC, Cohnstaedt LW, Fall K, Hamer GL. Emergence of Arboviruses in the United States: The Boom and Bust of Funding, Innovation, and Capacity. Trop Med Infect Dis 2020 Jun 6;5(2).
    doi: 10.3390/tropicalmed5020096pubmed: 32517268google scholar: lookup
26. Howard JJ, Oliver J, Dupuis Ii AP, Ngo KA, Stout J, Zink SD, Banker E, Maffei JG, Kramer LD, Sherwood JA, Ciota AT. Eastern equine encephalitis virus and identification of host bloodmeal sources from individual Culiseta melanura (Diptera: Culicidae) at an enzootic focus in central New York State. J Med Entomol 2026 Jan 20;63(1).
    doi: 10.1093/jme/tjaf147pubmed: 41144318google scholar: lookup
27. Bandyopadhyay A, Williamson LE, Sirohi D, Bailey K, Gilliland T Jr, Klose T, Buda G, Trivette A, Sun C, Julander JG, Klimstra WB, Crowe JE Jr, Kuhn RJ. Structural elucidation of a unique binding mode by an intact alphavirus human IgG molecule to a quaternary epitope. Nat Commun 2025 Aug 19;16(1):7716.
    doi: 10.1038/s41467-025-60505-xpubmed: 40830099google scholar: lookup
28. Fassola LA, Rupil LL, Martínez F, Albrieu-Llinás G, Serradell MDC. Induction of systemic and mucosal immune response against Zika virus by vaccination with non-infectious chimeric VLPs. Sci Rep 2025 Jul 10;15(1):24834.
    doi: 10.1038/s41598-025-07059-6pubmed: 40640219google scholar: lookup
29. Larkin CI, Dunn MD, Shoemaker JE, Klimstra WB, Faeder JR. A detailed kinetic model of Eastern equine encephalitis virus replication in a susceptible host cell. PLoS Comput Biol 2025 Jun;21(6):e1013082.
    doi: 10.1371/journal.pcbi.1013082pubmed: 40465541google scholar: lookup
30. de Souza WM, Lecuit M, Weaver SC. Chikungunya virus and other emerging arthritogenic alphaviruses. Nat Rev Microbiol 2025 Sep;23(9):585-601.
    doi: 10.1038/s41579-025-01177-8pubmed: 40335675google scholar: lookup
31. Padda H, Huang CY, Grimm K, Biggerstaff BJ, Ledermann JP, Raetz J, Boroughs K, Mossel EC, Martin SW, Lehman JA, Townsend RL, Krysztof D, Saá P, Dinh ETN, Stobierski MG, Esponda-Morrison B, Wolujewicz KAA, Osborne M, Brown CM, Hopkins B, Schiffman EK, Garvin A, Lee X, Osborn RA, Wozniak RJ, Brault AC, Basavaraju SV, Stramer SL, Staples JE, Gould CV. Powassan and Eastern Equine Encephalitis Virus Seroprevalence in Endemic Areas, United States, 2019-2020. Emerg Infect Dis 2025 May;31(5):929-936.
    doi: 10.3201/eid3105.240893pubmed: 40305306google scholar: lookup
32. Kordowitzki P. Eastern Equine Encephalitis Virus: The Importance of Metabolism and Aging. Int J Mol Sci 2024 Dec 12;25(24).
    doi: 10.3390/ijms252413318pubmed: 39769082google scholar: lookup
33. Larkin CI, Dunn MD, Shoemaker JE, Klimstra WB, Faeder JR. A detailed kinetic model of Eastern equine encephalitis virus replication in a susceptible host cell. bioRxiv 2024 Dec 26;.
    doi: 10.1101/2024.12.13.628424pubmed: 39764060google scholar: lookup
34. VanderGiessen M, de Jager C, Leighton J, Xie H, Theus M, Johnson E, Kehn-Hall K. Neurological manifestations of encephalitic alphaviruses, traumatic brain injuries, and organophosphorus nerve agent exposure. Front Neurosci 2024;18:1514940.
    doi: 10.3389/fnins.2024.1514940pubmed: 39734493google scholar: lookup
35. Mader EM, Clements N, Lehane Á, Gangloff-Kaufmann J, Crans S, Horton C, Greiner Safi A. A qualitative analysis of perceived risks and benefits of mosquito abatement and bite prevention strategies in Northeastern U.S. communities. J Med Entomol 2025 Mar 17;62(2):435-448.
    doi: 10.1093/jme/tjae144pubmed: 39548768google scholar: lookup
36. Merten EM, Sears JD, Leisner TM, Hardy PB, Ghoshal A, Hossain MA, Asressu KH, Brown PJ, Tse EG, Stashko MA, Li K, Norris-Drouin JL, Herring LE, Mordant AL, Webb TS, Mills CA, Barker NK, Streblow ZJ, Perveen S, Arrowsmith CH, Couñago RM, Arnold JJ, Cameron CE, Streblow DN, Moorman NJ, Heise MT, Willson TM, Popov KI, Pearce KH. Identification of a cell-active chikungunya virus nsP2 protease inhibitor using a covalent fragment-based screening approach. Proc Natl Acad Sci U S A 2024 Oct 15;121(42):e2409166121.
    doi: 10.1073/pnas.2409166121pubmed: 39388272google scholar: lookup
37. Cao D, Ma B, Cao Z, Xu X, Zhang X, Xiang Y. The receptor VLDLR binds Eastern Equine Encephalitis virus through multiple distinct modes. Nat Commun 2024 Aug 10;15(1):6866.
    doi: 10.1038/s41467-024-51293-xpubmed: 39127734google scholar: lookup
38. Beddingfield BJ, Plante KS, Plante JA, Weaver SC, Bose S, Krzykwa C, Chirichella N, Redmann RK, Seiler SZ, Dufour J, Blair RV, Endt K, Volkmann A, Maness NJ, Roy CJ. MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques. NPJ Vaccines 2024 Feb 27;9(1):47.
    doi: 10.1038/s41541-024-00842-ypubmed: 38413593google scholar: lookup
39. Nguyen TL, Kim H. Designing a Multiepitope Vaccine against Eastern Equine Encephalitis Virus: Immunoinformatics and Computational Approaches. ACS Omega 2024 Jan 9;9(1):1092-1105.
    doi: 10.1021/acsomega.3c07322pubmed: 38222668google scholar: lookup
40. Cao X, Yang D, Parvathareddy J, Chu YK, Kim EJ, Fitz-Henley JN, Li X, Lukka PB, Parmar KR, Temrikar ZH, Dhole P, Adcock RS, Gabbard J, Bansal S, Lee J, Zalduondo L, Hayes E, Stabenow J, Meibohm B, Fitzpatrick EA, Bailey K, Campos RK, Julander JG, Rossi SL, Chung D, Jonsson CB, Golden JE. Efficacy of a brain-penetrant antiviral in lethal Venezuelan and eastern equine encephalitis mouse models. Sci Transl Med 2023 Apr 12;15(691):eabl9344.
    doi: 10.1126/scitranslmed.abl9344pubmed: 37043558google scholar: lookup
41. Burtis JC, Poggi JD, Duval TB, Bidlack E, Shepard JJ, Matton P, Rossetti R, Harrington LC. Evaluation of a Methoprene Aerial Application for the Control of Culiseta melanura (Diptera: Culicidae) in Wetland Larval Habitats. J Med Entomol 2021 Nov 9;58(6):2330-2337.
    doi: 10.1093/jme/tjab108pubmed: 34144601google scholar: lookup