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BMC genomics2016; 17(1); 831; doi: 10.1186/s12864-016-3168-2

Novel equine tissue miRNAs and breed-related miRNA expressed in serum.

Abstract: MiRNAs regulate multiple genes at the post-transcriptional level and therefore play an important role in many biological processes. It has been suggested that miRNA exported outside the cells contribute to inter-cellular communication. Consequently, circulating miRNAs are of particular interest and are promising biomarkers for many diseases. The number of miRNAs annotated in the horse genome is much lower compared to model organisms like human and mouse. We therefore aimed to identify novel equine miRNAs for tissue types and breed in serum. We analysed 71 small RNA-seq libraries derived from nine tissues (gluteus medius, platysma, masseter muscle, heart, liver, cartilage, bone, total blood and serum) using miRDeep2 and miRdentify tools. Known miRNAs represented between 2.3 and 62.9 % of the reads in 71 libraries. A total of 683 novel miRNAs were identified. Breed and tissue type affected the number of miRNAs detected and interestingly, affected its average intensity. A total of 50 miRNAs in serum proved to be potential biomarkers to differentiate specific breed types, of which miR-122, miR-200, miR-483 were over-expressed and miR-328 was under-expressed in ponies compared to Warmbloods. The different miRNAs profiles, as well as the differences in their expression levels provide a foundation for more hypotheses based on the novel miRNAs discovered. We identified 683 novel equine miRNAs expressed in seven solid tissues, blood and serum. Additionally, our approach evidenced that such data supported identification of specific miRNAs as markers of functions related to breeds or disease tissues.
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Publication Date: 2016-10-26 PubMed ID: 27782799PubMed Central: PMC5080802DOI: 10.1186/s12864-016-3168-2Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aims to identify new microRNAs (miRNAs) in horse tissue and understand how they vary in serum between different horse breeds. The study also examined how these miRNAs might serve as biomarkers for various diseases.

MicroRNAs Role and Interest

  • MiRNAs are molecules that regulate gene expression at the post-transcriptional level, influencing various biological processes.
  • The theory that miRNAs can be exported out of cells to contribute to communication between cells makes circulating miRNAs a subject of interest in scientific research.
  • Such circulating miRNAs are seen as potential biomarkers for several diseases.

Background and Aim of the Study

  • The number of miRNAs recorded in the horse genome is far less than in more thoroughly studied organisms like humans and mice.
  • This research aimed to identify new horse-specific miRNAs in tissue types and breed in serum to narrow this knowledge gap.

Methodology

  • The team analyzed 71 small RNA-seq libraries extracted from nine different tissues using the tools miRDeep2 and miRdentify.
  • The tissues examined were the gluteus medius, platysma, masseter muscle, heart, liver, cartilage, bone, total blood, and serum.

Findings

  • From 2.3 to 62.9% of the reads in the 71 libraries were known miRNAs.
  • The researchers identified a total of 683 new miRNAs.
  • Both the breed and the tissue type influenced the number of miRNAs detected and their average intensity.
  • 50 miRNAs in serum were identified as potential biomarkers to differentiate specific horse breeds.
  • Among these, miR-122, miR-200, and miR-483 were found to be over-expressed, whereas miR-328 was found to be under-expressed in ponies compared to Warmbloods.

Conclusion and Implications of the Study

  • The discovery of new miRNAs and differences in their expression creates the foundation for forming new hypotheses based on these newly-discovered miRNAs.
  • These findings could contribute to identifying specific miRNAs as markers of functions related to animal breeds or disease tissues.

Cite This Article

APA
Pacholewska A, Mach N, Mata X, Vaiman A, Schibler L, Barrey E, Gerber V. (2016). Novel equine tissue miRNAs and breed-related miRNA expressed in serum. BMC Genomics, 17(1), 831. https://doi.org/10.1186/s12864-016-3168-2

Publication

BMC genomics
ISSN: 1471-2164
NlmUniqueID: 100965258
Country: England
Language: English
Volume: 17
Issue: 1
Pages: 831
PII: 831

Researcher Affiliations

Pacholewska, Alicja
  • Department of Clinical Veterinary Medicine, Swiss Institute of Equine Medicine, Vetsuisse Faculty, University of Bern, and Agroscope, Länggassstrasse 124, 3012, Bern, Switzerland. alicja.pacholewska@vetsuisse.unibe.ch.
  • Department of Clinical Research and Veterinary Public Health, Institute of Genetics, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109A, 3012, Bern, Switzerland. alicja.pacholewska@vetsuisse.unibe.ch.
Mach, Núria
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, University of Paris-Saclay, 78350, Jouy-en-Josas, France.
Mata, Xavier
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, University of Paris-Saclay, 78350, Jouy-en-Josas, France.
Vaiman, Anne
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, University of Paris-Saclay, 78350, Jouy-en-Josas, France.
Schibler, Laurent
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, University of Paris-Saclay, 78350, Jouy-en-Josas, France.
Barrey, Eric
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, University of Paris-Saclay, 78350, Jouy-en-Josas, France.
Gerber, Vincent
  • Department of Clinical Veterinary Medicine, Swiss Institute of Equine Medicine, Vetsuisse Faculty, University of Bern, and Agroscope, Länggassstrasse 124, 3012, Bern, Switzerland.

MeSH Terms

  • Animals
  • Base Sequence
  • Biomarkers
  • Breeding
  • Chromosome Mapping
  • Gene Expression Profiling / methods
  • Gene Expression Regulation
  • High-Throughput Nucleotide Sequencing
  • Horses / blood
  • Horses / genetics
  • MicroRNAs / blood
  • MicroRNAs / genetics
  • Nucleic Acid Conformation
  • Organ Specificity / genetics
  • Workflow

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