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Equine veterinary journal2021; 54(1); 132-138; doi: 10.1111/evj.13420

Novel seM-types of Streptococcus equi subsp. equi identified in isolates circulating in Argentina.

Abstract: Strangles is a worldwide infectious disease caused by Streptococcus equi subsp. equi that affects the upper respiratory tract of horses. Streptococcus equi subsp. equi characterisation by seM-typing is internationally used for epidemiological studies and comparison of isolates. Objective: To identify and to compare the seM-types of Argentinian isolates of Streptococcus equi subsp. equi. Methods: Investigation of bacterial isolates using molecular and phylogenetic approaches. Methods: A total of 59 Argentinian isolates of Streptococcus equi subsp. equi obtained between 2007 and 2019 were studied by seM-typing. The sequence similarity of Argentinian seM-types and the other alleles available on the seM database was determined using BLAST and phylogenetic analysis was performed using the Neighbour-Joining algorithm. The amino acid sequences were predicted and compared with the predicted amino acid sequence of the reference strain 4047 using the MEGA 7 software and PROVEAN tool. Results: Eight seM-types were found among the isolates. Only one of them (seM-61) has been previously reported and the other seven alleles (seM-129, seM-130, seM-131, seM-132, seM-133, seM-134 and seM-135) were novel seM sequences. High genetic similarity was observed among the Argentinian seM-types, with the exception of seM-130. No functional effects of amino acid differences were predicted. Conclusions: The number of related and unrelated isolates per year. Conclusions: Seven novel seM-types and seM-61 that were previously reported in Brazil were circulating in Argentina which were identified as circulating in Argentinian horses between 2007 and 2019. The high genetic similarity among the Argentinian and Brazilian seM-types suggests that there is a geographical distribution of strain types. The geographical restriction of strains is likely to reflect the movement of horses between different equine disciplines and neighbouring countries.
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Publication Date: 2021-02-23 PubMed ID: 33420757DOI: 10.1111/evj.13420Google Scholar: Lookup
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Summary

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The article presents research on the identification and comparison of various forms of the bacteria Streptococcus equi subsp. equi, which causes an infectious respiratory disease called strangles in horses. The study, focused on samples from Argentina, discovered seven new variants of the bacteria referred to as seM-types.

Research Methodology

  • The study was conducted on 59 Argentinian isolates of Streptococcus equi subsp. equi collected between 2007 and 2019. The isolates were characterised by using seM-typing, a globally recognised technique for bacteriological studies.
  • The researchers compared the similarity of the identified seM-types with existing data in the seM database. They used a software tool called BLAST for this purpose and carried out a phylogenetic analysis employing the Neighbour-Joining algorithm.
  • The potential amino acid sequences were predicted and contrasted with the sequence of a reference strain (4047), using tools like MEGA 7 and PROVEAN.

Obtained Results

  • Eight different seM-types were identified among the studied isolates. Out of these, only one (seM-61) had been documented before, while the other seven (seM-129, 130, 131, 132, 133, 134, and 135) were new variants.
  • The researchers observed a significant genetic similarity among the Argentinian seM-types, except for seM-130, suggesting a geographical distribution of these strain types.
  • Furthermore, the analysis revealed that differences in the amino acid sequences did not bring any functional effects.

Major Conclusions

  • Seven new seM-types and the previously reported seM-61 were present in the Argentinian samples studied from 2007 to 2019, showing that these types were circulating among the horse population in Argentina.
  • The high genetic similarity among the Argentinian and Brazilian seM-types could likely be reflecting the movement of horses between different equine disciplines within and between neighbouring countries.
  • The study, thus, gives an insight into the geographical restriction of the bacterial strains and its correlation with equine mobility.

Cite This Article

APA
Bustos CP, Muñoz AJ, Guida N, Waller A, Mesplet M. (2021). Novel seM-types of Streptococcus equi subsp. equi identified in isolates circulating in Argentina. Equine Vet J, 54(1), 132-138. https://doi.org/10.1111/evj.13420

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 54
Issue: 1
Pages: 132-138

Researcher Affiliations

Bustos, Carla P
  • Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Ciudad Autónoma de Buenos Aires, Argentina.
Muñoz, Alejandra J
  • Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Ciudad Autónoma de Buenos Aires, Argentina.
Guida, Nora
  • Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Ciudad Autónoma de Buenos Aires, Argentina.
Waller, Andrew
  • Intervacc AB, Hägersten, Sweden.
Mesplet, María
  • Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Ciudad Autónoma de Buenos Aires, Argentina.

MeSH Terms

  • Animals
  • Argentina / epidemiology
  • Horse Diseases / epidemiology
  • Horses
  • Phylogeny
  • Streptococcal Infections / epidemiology
  • Streptococcal Infections / veterinary
  • Streptococcus
  • Streptococcus equi / genetics

Grant Funding

  • UBACyT-20020130100299BA / Universidad de Buenos Aires
  • UBACyT-20020150200205BA / Universidad de Buenos Aires
  • UBACyT-20020170100537BA / Universidad de Buenos Aires
  • UBACyT-20020190200042BA / Universidad de Buenos Aires

References

This article includes 26 references
  1. Ministerio de Agricultura, Ganadería y Pesca, Argentina. https://www.magyp.gob.ar/sitio/areas/equinos/informacion_estadistica/. Accessed March 2020
  2. Servicio Nacional de Sanidad y Calidad Agroalimentaria, Argentina. http://www.senasa.gob.ar/cadena-animal/equinos. March 2020
  3. Waller S, Sellon D, Sweeney C, Timoney P, Newton R, Hines M. Streptococcal Infection. In: Sellon DC, Long MC, editors. Equine Infectious Diseases, (2nd edn). Missouri: Saunders Elsevier. pp 265-77.
  4. Newton JR, Wood JL, Dunn KA, DeBrauwere MN, Chanter N. Naturally occurring persistent and asymptomatic infection of the guttural pouches of horses with Streptococcus equi. Vet Rec 1997;140(4):84-90.
    doi: 10.1136/vr.140.4.84google scholar: lookup
  5. Cordoni G, Williams A, Durham A, Florio D, Zanoni RG, La Ragione RM. Rapid diagnosis of strangles (Streptococcus equi subspecies equi) using PCR. Res Vet Sci 2015;102:162-6.
    doi: 10.1016/j.rvsc.2015.08.008google scholar: lookup
  6. Webb K, Barker C, Harrison T, Heather Z, Steward KF, Robinson C. Detection of Streptococcus equi subspecies equi using a triplex qPCR assay. Vet J 2013;195(3):300-4.
    doi: 10.1016/j.tvjl.2012.07.007google scholar: lookup
  7. Båverud V, Johansson SK, Aspan A. Real-time PCR for detection and differentiation of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus. Vet Microbiol 2007;124(3-4):219-29.
    doi: 10.1016/j.vetmic.2007.04.020google scholar: lookup
  8. Timoney JF, Artiushin SC, Boschwitz JS. Comparison of the sequences and functions of Streptococcus equi M-like proteins SeM and SzPSe. Infect Immun 1997;65(9):3600-5.
    doi: 10.1128/iai.65.9.3600-3605.1997google scholar: lookup
  9. Anzai T, Kuwamoto Y, Wada R, Sugita S, Kakuda T, Takai S. Variation in the N-terminal region of an M-like protein of Streptococcus equi and evaluation of its potential as a tool in epidemiologic studies. Am J Vet Res 2005;66(12):2167-71.
    doi: 10.2460/ajvr.2005.66.2167google scholar: lookup
  10. Kelly C, Bugg M, Robinson C, Mitchell Z, Davis-Poynter N, Newton JR. Sequence variation of the SeM gene of Streptococcus equi allows discrimination of the source of strangles outbreaks. J Clin Microbiol 2006;44(2):480-6.
    doi: 10.1128/jcm.44.2.480-486.2006google scholar: lookup
  11. Bustos C, Guida N, Casamayor A, Muñoz A, Fernandez Garayzabal J, Vela AA. First report of molecular characterization of Argentine isolates of Streptococcus equi subsp. equi by pulsed-field gel electrophoresis. J Equine Vet Sci 2017;53(30):30-3.
    doi: 10.1016/j.jevs.2017.01.007google scholar: lookup
  12. Hall TA. BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
  13. Huang X, Madan A. CAP3: A DNA sequence assembly program. Genome Res 1999;9(9):868-77.
    doi: 10.1101/gr.9.9.868google scholar: lookup
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4(4):406-25.
    doi: 10.1093/oxfordjournals.molbev.a040454google scholar: lookup
  15. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol 2016;33(7):1870-4.
    doi: 10.1093/molbev/msw054google scholar: lookup
  16. Choi Y, Sims GE, Murphy S, Miller JR, Chan AP. Predicting the functional effect of amino acid substitutions and indels. PLoS One 2012;7(10):e46688.
    doi: 10.1371/journal.pone.0046688google scholar: lookup
  17. Libardoni F, Vielmo A, Farias L, Matter LB, Pötter L, Spilki FR. Diversity of seM in Streptococcus equi subsp. equi isolated from strangles outbreaks. Vet Microbiol 2013;162(2-4):663-9.
    doi: 10.1016/j.vetmic.2012.09.010google scholar: lookup
  18. Ivens PA, Matthews D, Webb K, Newton JR, Steward K, Waller AS. Molecular characterisation of 'strangles' outbreaks in the UK: the use of M-protein typing of Streptococcus equi ssp. equi. Equine Vet J 2011;43(3):359-64.
    doi: 10.1111/j.2042-3306.2010.00177.xgoogle scholar: lookup
  19. Lindahl S, Söderlund R, Frosth S, Pringle J, Båverud V, Aspán A. Tracing outbreaks of Streptococcus equi infection (strangles) in horses using sequence variation in the seM gene and pulsed-field gel electrophoresis. Vet Microbiol 2011;153(1):144-9.
    doi: 10.1016/j.vetmic.2011.03.027google scholar: lookup
  20. Lanka S, Borst LB, Patterson SK, Maddox CW. A multiphasic typing approach to subtype Streptococcus equi subspecies equi. J Vet Diagn Invest 2010;22(6):928-36.
    doi: 10.1177/104063871002200612google scholar: lookup
  21. Lindmark H, Jonsson P, Engvall E, Guss B. Pulsed-field gel electrophoresis and distribution of the genes zag and fnz in isolates of Streptococcus equi. Res Vet Sci 1999;66(2):93-9.
    doi: 10.1053/rvsc.1998.0250google scholar: lookup
  22. Bustos C, Muñoz A, Picos J, Moras E, Guida N. Different strains of Streptococcus equi subsp. equi isolated from a guttural pouch empyema. J Equine Vet Sci 2012;32:S19-S20.
  23. Harris SR, Robinson C, Steward KF, Webb KS, Paillot R, Parkhill J. Genome specialization and decay of the strangles pathogen, Streptococcus equi, is driven by persistent infection. Genome Res 2015;25(9):1360-71.
    doi: 10.1101/gr.189803.115google scholar: lookup
  24. Libardoni F, Machado G, Gressler LT, Kowalski AP, Diehl GN, dos Santos LC. Prevalence of Streptococcus equi subsp. equi in horses and associated risk factors in the State of Rio Grande do Sul, Brazil. Res Vet Sci 2016;104:53-7.
    doi: 10.1016/j.rvsc.2015.11.009google scholar: lookup
  25. Moloney E, Kavanagh KS, Buckley TC, Cooney JC. Lineages of Streptococcus equi ssp. equi in the Irish equine industry. Ir Vet J 2013;66(1):10-.
    doi: 10.1186/2046-0481-66-10google scholar: lookup
  26. Galán JE, Timoney JF. Immunologic and genetic comparison of Streptococcus equi isolates from the United States and Europe. J Clin Microbiol 1988;26(6):1142-6.
    doi: 10.1128/jcm.26.6.1142-1146.1988google scholar: lookup

Citations

This article has been cited 1 times.
  1. Bekele D, Dessalegn B, Tadesse B, Abey SL. Streptococcus equi subspecies equi from strangles suspected equines: molecular detection, antibiogram profiles and risk factors. BMC Vet Res 2024 Aug 23;20(1):377.
    doi: 10.1186/s12917-024-04236-zpubmed: 39180060google scholar: lookup
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