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Home / Equine Research Bank / Equine Vet J / Novel seM-types of Streptococcus equi subsp. equi identified...
Equine veterinary journal2021; 54(1); 132-138; doi: 10.1111/evj.13420

Novel seM-types of Streptococcus equi subsp. equi identified in isolates circulating in Argentina.

Abstract: Strangles is a worldwide infectious disease caused by Streptococcus equi subsp. equi that affects the upper respiratory tract of horses. Streptococcus equi subsp. equi characterisation by seM-typing is internationally used for epidemiological studies and comparison of isolates. Objective: To identify and to compare the seM-types of Argentinian isolates of Streptococcus equi subsp. equi. Methods: Investigation of bacterial isolates using molecular and phylogenetic approaches. Methods: A total of 59 Argentinian isolates of Streptococcus equi subsp. equi obtained between 2007 and 2019 were studied by seM-typing. The sequence similarity of Argentinian seM-types and the other alleles available on the seM database was determined using BLAST and phylogenetic analysis was performed using the Neighbour-Joining algorithm. The amino acid sequences were predicted and compared with the predicted amino acid sequence of the reference strain 4047 using the MEGA 7 software and PROVEAN tool. Results: Eight seM-types were found among the isolates. Only one of them (seM-61) has been previously reported and the other seven alleles (seM-129, seM-130, seM-131, seM-132, seM-133, seM-134 and seM-135) were novel seM sequences. High genetic similarity was observed among the Argentinian seM-types, with the exception of seM-130. No functional effects of amino acid differences were predicted. Conclusions: The number of related and unrelated isolates per year. Conclusions: Seven novel seM-types and seM-61 that were previously reported in Brazil were circulating in Argentina which were identified as circulating in Argentinian horses between 2007 and 2019. The high genetic similarity among the Argentinian and Brazilian seM-types suggests that there is a geographical distribution of strain types. The geographical restriction of strains is likely to reflect the movement of horses between different equine disciplines and neighbouring countries.
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Publication Date: 2021-02-23 PubMed ID: 33420757DOI: 10.1111/evj.13420Google Scholar: Lookup
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Summary

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The article presents research on the identification and comparison of various forms of the bacteria Streptococcus equi subsp. equi, which causes an infectious respiratory disease called strangles in horses. The study, focused on samples from Argentina, discovered seven new variants of the bacteria referred to as seM-types.

Research Methodology

  • The study was conducted on 59 Argentinian isolates of Streptococcus equi subsp. equi collected between 2007 and 2019. The isolates were characterised by using seM-typing, a globally recognised technique for bacteriological studies.
  • The researchers compared the similarity of the identified seM-types with existing data in the seM database. They used a software tool called BLAST for this purpose and carried out a phylogenetic analysis employing the Neighbour-Joining algorithm.
  • The potential amino acid sequences were predicted and contrasted with the sequence of a reference strain (4047), using tools like MEGA 7 and PROVEAN.

Obtained Results

  • Eight different seM-types were identified among the studied isolates. Out of these, only one (seM-61) had been documented before, while the other seven (seM-129, 130, 131, 132, 133, 134, and 135) were new variants.
  • The researchers observed a significant genetic similarity among the Argentinian seM-types, except for seM-130, suggesting a geographical distribution of these strain types.
  • Furthermore, the analysis revealed that differences in the amino acid sequences did not bring any functional effects.

Major Conclusions

  • Seven new seM-types and the previously reported seM-61 were present in the Argentinian samples studied from 2007 to 2019, showing that these types were circulating among the horse population in Argentina.
  • The high genetic similarity among the Argentinian and Brazilian seM-types could likely be reflecting the movement of horses between different equine disciplines within and between neighbouring countries.
  • The study, thus, gives an insight into the geographical restriction of the bacterial strains and its correlation with equine mobility.

Cite This Article

APA
Bustos CP, Muñoz AJ, Guida N, Waller A, Mesplet M. (2021). Novel seM-types of Streptococcus equi subsp. equi identified in isolates circulating in Argentina. Equine Vet J, 54(1), 132-138. https://doi.org/10.1111/evj.13420

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 54
Issue: 1
Pages: 132-138

Researcher Affiliations

Bustos, Carla P
  • Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Ciudad Autónoma de Buenos Aires, Argentina.
Muñoz, Alejandra J
  • Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Ciudad Autónoma de Buenos Aires, Argentina.
Guida, Nora
  • Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Ciudad Autónoma de Buenos Aires, Argentina.
Waller, Andrew
  • Intervacc AB, Hägersten, Sweden.
Mesplet, María
  • Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Ciudad Autónoma de Buenos Aires, Argentina.

MeSH Terms

  • Animals
  • Argentina / epidemiology
  • Horse Diseases / epidemiology
  • Horses
  • Phylogeny
  • Streptococcal Infections / epidemiology
  • Streptococcal Infections / veterinary
  • Streptococcus
  • Streptococcus equi / genetics

Grant Funding

  • UBACyT-20020130100299BA / Universidad de Buenos Aires
  • UBACyT-20020150200205BA / Universidad de Buenos Aires
  • UBACyT-20020170100537BA / Universidad de Buenos Aires
  • UBACyT-20020190200042BA / Universidad de Buenos Aires

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Citations

This article has been cited 1 times.
  1. Bekele D, Dessalegn B, Tadesse B, Abey SL. Streptococcus equi subspecies equi from strangles suspected equines: molecular detection, antibiogram profiles and risk factors. BMC Vet Res 2024 Aug 23;20(1):377.
    doi: 10.1186/s12917-024-04236-zpubmed: 39180060google scholar: lookup
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