Occurrence and characterization of KPC-2-producing ST11 Klebsiella pneumoniae isolate and NDM-5-producing Escherichia coli isolate from the same horse of equestrian clubs in China.
Abstract: Carbapenem-resistant Enterobacteriaceae (CRE) have been rapidly increasing among animals in many countries and have been a great threat to public health. Horse riding is becoming increasingly popular worldwide; however, reports of CRE producing NDM or KPC-2, two prevalent types of carbapenemases, from horses of equestrian club are extremely scarce and KPC-2-producing Klebsiella pneumoniae in animals is still rarely characterized. In this study, we identified four NDM-5-producing Escherichia coli isolates from horses in equestrian club in Qingdao, China, and one horse possessing NDM-5-producing E. coli also carried ST11 KPC-2-producing K. pneumoniae. Transferability of the plasmids producing carbapenemases was determined by conjugation, and the sequences of all CRE isolates and their transconjugants were then analysed by using whole-genome sequencing. bla was located on a highly similar ~ 46 kb self-transmissible IncX3 plasmid in all isolates, and these plasmids were nearly identical to IncX3 plasmids from different bacterial species of clinical patients in several countries, even including plasmid from clinical E. coli in Qingdao, China. The chromosome of the ST11 K. pneumoniae in this study was highly similar to ST11 clinical K. pneumoniae reported worldwide including the ST11 KPC-2-producing WCHKP020098 from Chengdu, China, and the bla -bearing plasmid in our study was a novel F33:A-:B- non-conjugative multidrug resistance plasmid. The presence of CRE from horses in equestrian club is alarming due to the potential for transmitting these isolates to humans during horse riding, and the prevalence of CRE among equestrian clubs in the whole country requires further monitoring.
© 2020 Blackwell Verlag GmbH.
Publication Date: 2020-05-19 PubMed ID: 32386080DOI: 10.1111/tbed.13614Google Scholar: Lookup
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- Journal Article
Summary
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The study shows the detection and characterization of two types of serious bacterial infections in horses from an equestrian club in China, each caused by different strains of Carbapenem-resistant Enterobacteriaceae (CRE). These bacteria possess certain types of enzymes that make them resistant to carbapenem antibiotics.
About the Study
- The researchers focused on characterizing Klebsiella pneumoniae producing KPC-2 and Escherichia coli producing NDM-5. These strains are nearly non-existent in reports related to horse-borne infections, with KPC-2-producing Klebsiella pneumoniae rarely characterized in animals in particular.
- In their study, they identified four NDM-5-producing Escherichia coli isolates and one ST11 KPC-2-producing K. pneumoniae strain in horses at an equestrian club in Qingdao, China.
- Their method involved the use of conjugation to determine if the carbapenemase-producing plasmids transfer from one bacterium to another. Following this, they analyzed the sequences of the observed CRE pathogens using whole-genome sequencing.
Key Findings
- The research discovered that the “bla” gene, which confers resistance to beta-lactam antibiotics, was found on a highly similar self-transmissible IncX3 plasmid in all isolates. This showed that the plasmid could spread bacterial resistance among different bacterial species.
- Interestingly, these plasmids were virtually identical to IncX3 plasmids found in different bacterial species known to infect clinical patients in numerous countries. This includes an E. coli strain detected in Qingdao, China.
- The chromosome of the identified ST11 K. pneumoniae was found to be highly similar to ST11 clinical K. pneumoniae cases reported worldwide, suggesting a common origin or pathway. Furthermore, the bla gene, in this case, was found on a novel F33:A-:B- non-conjugative multidrug resistance plasmid.
Implications
- The existence of CRE in horses, particularly within equestrian clubs, raises serious public health concerns due to the potential for these strains to be transmitted to humans during horse riding.
- Due to the severity and increase of such findings, the researchers recommend that the prevalence of these infections be thoroughly monitored within equestrian clubs across the entire country.
Cite This Article
APA
Wang H, Li X, Liu BT.
(2020).
Occurrence and characterization of KPC-2-producing ST11 Klebsiella pneumoniae isolate and NDM-5-producing Escherichia coli isolate from the same horse of equestrian clubs in China.
Transbound Emerg Dis, 68(2), 224-232.
https://doi.org/10.1111/tbed.13614 Publication
Researcher Affiliations
- College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
- College of Agricultural, Liaocheng University, Liaocheng, China.
- College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
MeSH Terms
- Animals
- Carbapenem-Resistant Enterobacteriaceae / enzymology
- Carbapenem-Resistant Enterobacteriaceae / isolation & purification
- China
- Escherichia coli / enzymology
- Escherichia coli / isolation & purification
- Escherichia coli Infections / microbiology
- Escherichia coli Infections / veterinary
- Horse Diseases / microbiology
- Horses
- Klebsiella Infections / microbiology
- Klebsiella Infections / veterinary
- Klebsiella pneumoniae / enzymology
- Klebsiella pneumoniae / isolation & purification
- beta-Lactamases / metabolism
Grant Funding
- 2019JZZY010719 / Shandong Major Science and Technology Innovation Projects
- 19-6-1-94-nsh / Scientific and Technological Projects of Qingdao
- 31502122 / National Natural Science Foundation of China
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Citations
This article has been cited 3 times.- Li R, Zhang L, Lu X, Peng K, Liu Y, Xiao X, Song H, Wang Z. Occurrence and Characterization of NDM-1-Producing Shewanella spp. and Acinetobacter portensis Co-Harboring tet(X3) in a Chinese Dairy Farm.. Antibiotics (Basel) 2022 Oct 17;11(10).
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