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Home / Equine Research Bank / J Eukaryot Microbiol / Occurrence and Genetic Characteristics of Cryptosporidium ho...
The Journal of eukaryotic microbiology2017; 64(5); 716-720; doi: 10.1111/jeu.12399

Occurrence and Genetic Characteristics of Cryptosporidium hominis and Cryptosporidium andersoni in Horses from Southwestern China.

Abstract: A total of 333 fecal specimens from horses in southwestern China were genotyped based on analysis of the small subunit rRNA (SSU rRNA) gene. Cryptosporidium hominis and Cryptosporidium andersoni were identified in 2 and 4 stool specimens, respectively. The identification of C. hominis was confirmed by sequence analysis of the 70-kDa heat shock protein (HSP70) and oocyst wall protein (COWP) genes. Subtyping analysis of the 60-kDa glycoprotein (GP60) gene sequence of C. hominis revealed a new rare subtype Id, named IdA15; only three Id isolates have been reported in humans to date. Multilocus sequence typing (MLST) analysis indicated that the C. andersoni subtype was A6, A5, A2, and A1 at the four minisatellite loci (MS1, MS2, MS3, and MS16, respectively). This is the first report to identify the presence of C. andersoni and C. hominis in horses in southwestern China and the first to identify a rare zoonotic subtype Id of C. hominis in horses. These findings suggest that infected horses may act as potential reservoirs of Cryptosporidium to transmit infections to humans.
© 2017 The Authors Journal of Eukaryotic Microbiology published by Wiley Periodicals, Inc. on behalf of International Society of Protistologists.
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Publication Date: 2017-03-08 PubMed ID: 28166378PubMed Central: PMC5599969DOI: 10.1111/jeu.12399Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the prevalence and genetic characteristics of particular parasites, Cryptosporidium hominis and Cryptosporidium andersoni, in horse fecal samples in southwestern China. The study offers the first identification of these parasites in this region’s horses, revealing their potential role as infection reservoirs for human transmission.

Methodology and Sample Analysis

  • The researchers collected 333 fecal specimens from horses in southwestern China. These samples underwent genotyping, specifically analyzing the small subunit rRNA (SSU rRNA) gene.
  • The genotyping process revealed the presence of Cryptosporidium hominis and Cryptosporidium andersoni in 2 and 4 stool specimens respectively.
  • To confirm the presence of C. hominis, they conducted sequence analysis of the 70-kDa heat shock protein (HSP70) and oocyst wall protein (COWP) genes.

Genetic Characteristics and Subtyping

  • They further examined the 60-kDa glycoprotein (GP60) gene sequence of C. hominis, revealing a rare subtype Id, which they named IdA15.
  • Only three instances of Id isolates have been reported in humans to date. Hence, IdA15 represents a unique find.
  • They utilized Multilocus sequence typing (MLST) analysis for C. andersoni, which indicated subtypes A6, A5, A2, and A1 at the four minisatellite loci (MS1, MS2, MS3, and MS16, respectively).

Research Outcomes and Implications

  • This is the first study reporting the existence of C. andersoni and C. hominis in horses in the region of southwestern China.
  • The discovery of a rare zoonotic subtype Id of C. hominis in horses is particularly notable and has not been identified previously.
  • The findings imply that horses infected with these parasites may serve as potential reservoirs for Cryptosporidium transmission, thereby posing a risk to human health.

Cite This Article

APA
Deng L, Li W, Zhong Z, Gong C, Cao X, Song Y, Wang W, Huang X, Liu X, Hu Y, Fu H, He M, Wang Y, Zhang Y, Wu K, Peng G. (2017). Occurrence and Genetic Characteristics of Cryptosporidium hominis and Cryptosporidium andersoni in Horses from Southwestern China. J Eukaryot Microbiol, 64(5), 716-720. https://doi.org/10.1111/jeu.12399

Publication

The Journal of eukaryotic microbiology
ISSN: 1550-7408
NlmUniqueID: 9306405
Country: United States
Language: English
Volume: 64
Issue: 5
Pages: 716-720

Researcher Affiliations

Deng, Lei
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Li, Wei
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Zhong, Zhijun
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Gong, Chao
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Cao, Xuefeng
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Song, Yuan
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Wang, Wuyou
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Huang, Xiangming
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Liu, Xuehan
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Hu, Yanchun
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Fu, Hualin
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
He, Min
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Wang, Ya
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Zhang, Yue
  • Chengdu Giant Panda Breeding Research Base, Chengdu, Sichuan Province, 625001, China.
Wu, Kongju
  • Chengdu Giant Panda Breeding Research Base, Chengdu, Sichuan Province, 625001, China.
Peng, Guangneng
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.

MeSH Terms

  • Animals
  • China
  • Cryptosporidium / classification
  • Cryptosporidium / genetics
  • DNA, Protozoan / genetics
  • DNA, Ribosomal / genetics
  • Feces / parasitology
  • Genotyping Techniques
  • Horses / parasitology
  • Humans
  • Multilocus Sequence Typing
  • Phylogeny
  • Sequence Analysis, DNA / methods

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This article has been cited 13 times.
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