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Home / Equine Research Bank / Biomed Res Int / Occurrence of West Nile virus antibodies in wild birds, hors...
BioMed research international2015; 2015; 234181; doi: 10.1155/2015/234181

Occurrence of West Nile virus antibodies in wild birds, horses, and humans in Poland.

Abstract: Serum samples of 474 wild birds, 378 horses, and 42 humans with meningitis and lymphocytic meningitis were collected between 2010 and 2014 from different areas of Poland. West Nile virus (WNV) antibodies were detected using competition enzyme linked immunosorbent assays: ELISA-1 ID Screen West Nile Competition, IDvet, ELISA-2 ID Screen West Nile IgM Capture, and ELISA-3 Ingezim West Nile Compac. The antibodies were found in 63 (13.29%) out of 474 wild bird serum samples and in one (0.26%) out of 378 horse serum samples. Fourteen (33.33%) out of 42 sera from patients were positive against WNV antigen and one serum was doubtful. Positive samples obtained in birds were next retested with virus microneutralisation test to confirm positive results and cross-reactions with other antigens of the Japanese encephalitis complex. We suspect that positive serological results in humans, birds, and horses indicate that WNV can be somehow closely related with the ecosystem in Poland.
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Publication Date: 2015-03-19 PubMed ID: 25866767PubMed Central: PMC4383358DOI: 10.1155/2015/234181Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the detection of West Nile virus (WNV) antibodies in wild birds, horses, and humans in Poland between 2010 and 2014, suggesting the potential presence of the virus within the Polish ecosystem.

Sample Collection

  • For this study, researchers collected serum samples from a diverse range of subjects. These include 474 wild birds, 378 horses, and 42 humans who were diagnosed with meningitis and lymphocytic meningitis.
  • The samples were collected over a four-year period, from 2010 to 2014, across different regions in Poland.

Methodology and Results

  • The team utilized competition enzyme-linked immunosorbent assays (ELISAs) to check for the presence of WNV antibodies in the collected samples. Three types of ELISAs were used: ELISA-1 ID Screen West Nile Competition, IDvet, ELISA-2 ID Screen West Nile IgM Capture, and ELISA-3 Ingezim West Nile Compac.
  • The tests found antibodies in 63 out of the 474 wild bird samples (13.29%) and in one out of the 378 horse samples (0.26%).
  • From the enlisted human samples, 14 out of 42 (33.33%) were observed to be positive for WNV antigens and one serum sample yield a doubtful result.
  • The bird samples that gave a positive result were retested with a virus microneutralisation test. This step was taken to confirm the initially-obtained results, and to check for cross-reactions with other antigens from the Japanese encephalitis complex.

Conclusion and Implications

  • The study concluded that the positive serological results in wild birds, horses, and humans suggest the possibility of West Nile virus being present and closely interacting with the Polish ecosystem.
  • This kind of research is critical in understanding the spread and prevalence of viruses, such as WNV, and for formulating strategies for disease prevention and control.

Cite This Article

APA
Niczyporuk JS, Samorek-Salamonowicz E, Lecollinet S, Pancewicz SA, Kozdruń W, Czekaj H. (2015). Occurrence of West Nile virus antibodies in wild birds, horses, and humans in Poland. Biomed Res Int, 2015, 234181. https://doi.org/10.1155/2015/234181

Publication

BioMed research international
ISSN: 2314-6141
NlmUniqueID: 101600173
Country: United States
Language: English
Volume: 2015
Pages: 234181
PII: 234181

Researcher Affiliations

Niczyporuk, Jowita Samanta
  • Department of Poultry Viral Diseases, National Veterinary Research Institute Pulawy (NVRI), Al. Partyzantow 57, 24-100 Pulawy, Poland.
Samorek-Salamonowicz, Elżbieta
  • Department of Poultry Viral Diseases, National Veterinary Research Institute Pulawy (NVRI), Al. Partyzantow 57, 24-100 Pulawy, Poland.
Lecollinet, Sylvie
  • ANSES, Laboratoire de Santé Animale de Maisons-Alfort, UMR 1161 Virologie, INRA, ANSES, ENVA, 23 avenue du Général de Gaulle, Maisons-Alfort, 94706 Paris, France.
Pancewicz, Sławomir Andrzej
  • Department of Infectious Diseases and Neuroinfections, Medical University of Bialystok, 14 Żurawia Street, 15-540 Bialystok, Poland.
Kozdruń, Wojciech
  • Department of Poultry Viral Diseases, National Veterinary Research Institute Pulawy (NVRI), Al. Partyzantow 57, 24-100 Pulawy, Poland.
Czekaj, Hanna
  • Department of Poultry Viral Diseases, National Veterinary Research Institute Pulawy (NVRI), Al. Partyzantow 57, 24-100 Pulawy, Poland.

MeSH Terms

  • Animals
  • Antibodies, Viral / blood
  • Birds
  • Female
  • Horses
  • Humans
  • Male
  • Poland / epidemiology
  • West Nile Fever / blood
  • West Nile Fever / epidemiology
  • West Nile virus

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Citations

This article has been cited 14 times.
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