On the repair of oxidative damage to apoferritin: a model study with the flavonoids quercetin and rutin in aerated and deaerated solutions.
Abstract: Ferritin (Ft) impairment through (•)O2(-), H2O2, and (•)OH production occurs in the cases of ketoses, diabetes mellitus, acute intermittent porphyria and tyrosinemia. In addition to (•)Trp and TyrO(•) radical production, ferrous iron liberation and Ft synthesis stimulation, site-specific oxidation reactions are induced leading to toxic iron accumulation in organs with high Ft content, for example, liver and brain. To elucidate the potential pathways to Ft recovery, repair of oxidative damage to horse spleen apoferritin (apoFt) and Ft by quercetin (QH) or rutin (RH) was studied in the presence and absence of oxygen. (•)Trp and TyrO(•) radicals were produced in pulse radiolysis through apoFt oxidation by (•)Br2(-) radicals. QH and RH bind to apoFt on eight sites with binding constants of ˜80,000 and ˜32,000 M(-1), respectively. In deaerated solutions, a repair of apoFt radicals is observed involving both bound and free flavonoids. This repair occurs by a fast intra- and a slow inter-molecular electron transfer from bound and free flavonoids, respectively. With QH, the rate constants are 10(4) s(-1) and 3.5 × 10(7) M(-1) s(-1) for the intra- and intermolecular repair reactions, respectively. Oxygen does not interfere with repair of apoFt or Ft by bound QH but inhibits 90% of Ft repair by RH. These results taken together indicate that flavonoid antioxidants may help alleviate Ft impairment in diseases involving an oxidative stress.
Publication Date: 2013-05-02 PubMed ID: 23560676DOI: 10.3109/10715762.2013.791024Google Scholar: Lookup
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- Journal Article
Summary
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The study explores how flavonoids like quercetin and rutin may aid in repairing oxidative damage to ferritin, a type of protein that stores iron. This can be significant in managing conditions that involve oxidative stress and are known to impair ferritin, such as diabetes and ketoses.
Understanding The Research
- The researchers looked at the impairment of Ferritin (Ft), a protein that stores iron in the body, during cases of ketoses, diabetes mellitus, acute intermittent porphyria, and tyrosinemia. These conditions lead to the production of certain radicals – O2(-), H2O2, and OH – that are known to damage Ferritin.
- Specific oxidation reactions are also induced in these conditions, which can result in an unhealthy accumulation of iron, especially in organs with a high concentration of Ferritin, like liver and brain.
- The goal of the study is to understand the possible mechanisms for recovering Ferritin from such oxidative damage.
The Role of Flavonoids
- The researchers focused on the flavonoids quercetin (QH) and rutin (RH), studying the repair of oxidative damage to a type of Ferritin called horse spleen apoferritin (apoFt) in the presence and absence of oxygen.
- Both QH and RH were found to bind to the Ferritin on eight sites, and the repair of apoFt radicals was observed in solutions lacking oxygen.
- This repair seems to occur via a quick internal (intra-molecular) and a slower external (inter-molecular) electron transfer from both bound and free flavonoids (QH and RH).
Impact of Oxygen and Flavonoid Type
- Oxygen didn’t appear to interfere with the repair process when using bound QH.
- However, Oxygen seemed to inhibit 90% of Ferritin repair with rutin (RH), indicating differences in how these two flavonoids function in the presence of Oxygen.
The Potential of Flavonoids
- Overall, the study suggests that flavonoids, through their antioxidant properties, may help alleviate Ferritin impairment in diseases involving oxidative stress.
- This can potentially open up new avenues for managing such conditions, where damaging oxidative processes are at play.
Cite This Article
APA
Morlière P, Mazière JC, Patterson LK, Conte MA, Dupas JL, Ducroix JP, Filipe P, Santus R.
(2013).
On the repair of oxidative damage to apoferritin: a model study with the flavonoids quercetin and rutin in aerated and deaerated solutions.
Free Radic Res, 47(6-7), 463-473.
https://doi.org/10.3109/10715762.2013.791024 Publication
Researcher Affiliations
- INSERM, U1088, 80054 Amiens cedex, France. morliere.patrice@chu-amiens.fr
MeSH Terms
- Air
- Animals
- Antioxidants / chemistry
- Antioxidants / pharmacology
- Apoferritins / chemistry
- Apoferritins / metabolism
- Free Radicals / chemistry
- Free Radicals / metabolism
- Horses
- Models, Biological
- Oxidative Stress / drug effects
- Quercetin / chemistry
- Quercetin / pharmacology
- Rutin / chemistry
- Rutin / pharmacology
- Solutions
- Spleen / chemistry
Citations
This article has been cited 1 times.- Zaborowski MK, Długosz A, Błaszak B, Szulc J, Leis K. The Role of Quercetin as a Plant-Derived Bioactive Agent in Preventive Medicine and Treatment in Skin Disorders. Molecules 2024 Jul 5;29(13).
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