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Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes.
Abstract: Previous studies in the horse highlight the potential benefit of prolonged in vitro maturation (IVM) (34 h) compared to short IVM (24 h) with or without prior oocyte holding, but little is known about the optimal IVM duration within this interval. To determine the effect of oocyte holding and duration of IVM ranged between 28 and 34 h on nuclear maturation, cleavage, blastocyst formation, and pregnancy rates, a retrospective study was performed in an equine clinical OPU-ICSI setting. The study included data of 2114 aspirated oocytes from 201 OPU-ICSI sessions. Duration of IVM was divided in three different time windows using quartiles, with 465 oocytes (22.0 %) between 28 and 30 h (first quartile), 1078 oocytes (51.0 %) >30 and 31.7 h (second and third quartiles), and 571 oocytes (27.0 %) >31.7 and 34 h (fourth quartile). Using logistic regression models, the effect of duration of IVM with and without holding was tested on nuclear maturation, cleavage, blastocyst, and pregnancy rates. The three IVM intervals did not show differences in nuclear maturation (respectively 64.5 ± 0.48 %, 65.7 ± 0.47 %, and 67.3 ± 0.47 %), cleavage (respectively 59.7 ± 0.49 %, 58.5 ± 0.49 %, and 64.8 ± 0.48 %), blastocyst (respectively 17.5 ± 0.38 %, 19.0 ± 0.39 %, and 20.8 ± 0.41 %) nor pregnancy rates (respectively 65.4 ± 0.49 %, 70.3 ± 0.46 %, and 74.2 % ± 0.44) (P ≥ 0.38). Oocyte holding prior to IVM did not affect the results either (P ≥ 0.15). In conclusion, oocyte holding and IVM duration between 28 and 34h do not significantly affect outcomes, allowing flexibility in the planning of clinical OPU-ICSI in horses.
Copyright © 2024 Elsevier Inc. All rights reserved.
Publication Date: 2024-11-22 PubMed ID: 39608306DOI: 10.1016/j.theriogenology.2024.11.019Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This research paper explores the impact of in-vitro maturation (IVM) duration on horse oocyte outcomes, studying durations of 28 to 34 hours. The study found no significant effect of the duration of IVM or pre-maturation oocyte holding on key performance indicators such as nuclear maturation, blastocyst formation, cleavage, and pregnancy rates, allowing for more flexibility in clinical OPU-ICSI scheduling in horses.
Study Design
- The researchers conducted a retrospective study in a clinical equine oocyte pick-up (OPU) and intracytoplasmic sperm injection (ICSI) setting. This involves aspirating oocytes (eggs) from the ovaries, maturing them in-vitro (outside the body), and directly injecting sperm into the oocyte.
- It included data from 201 OPU-ICSI sessions, resulting in 2114 aspirated oocytes.
- The duration of IVM was segmented into three different time windows using quartiles, each representing different groups with varied IVM duration.
Methods
- To test the impact of IVM duration on the performance measures, logistic regression models were used. This provided an association between the IVM duration (with and without holding) and outcomes.
- Nuclear maturation, cleavage, formation of blastocyst, and pregnancy rates were the outcomes measured.
Results
- The results indicated no significant differences in nuclear maturation, cleavage, blastocyst, or pregnancy rates across the three time windows for IVM.
- Even the practice of oocyte holding prior to IVM did not affect the results, thus indicating no effect on the outcome measures.
Conclusion
- The paper concluded that the duration of in-vitro maturation (between 28 and 34 hours) and prior holding of oocytes do not significantly impact the outcomes of equine OPU-ICSI sessions.
- This opens up the possibility for greater flexibility and less time-sensitive planning in clinical equine OPU-ICSI, thereby potentially reducing the strain on resources and practitioners.
Cite This Article
APA
Broothaers K, Pascottini OB, Hedia M, Angel-Velez D, De Coster T, Peere S, Polfliet E, Van den Branden E, Govaere J, Van Soom A, Smits K.
(2024).
Oocyte holding and in vitro maturation duration between 28 and 34 hours do not affect equine OPU-ICSI outcomes.
Theriogenology, 233, 64-69.
https://doi.org/10.1016/j.theriogenology.2024.11.019 Publication
Researcher Affiliations
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium. Electronic address: Klaartje.broothaers@ugent.be.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium; School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium; Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Gamete Research Centre, Laboratory for Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium; School of Veterinary Medicine, Universidad CES, Medellin, Colombia.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
- Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
MeSH Terms
- Animals
- Horses / physiology
- Female
- In Vitro Oocyte Maturation Techniques / veterinary
- In Vitro Oocyte Maturation Techniques / methods
- Sperm Injections, Intracytoplasmic / veterinary
- Sperm Injections, Intracytoplasmic / methods
- Pregnancy
- Retrospective Studies
- Oocytes / physiology
- Time Factors
- Pregnancy Rate
Conflict of Interest Statement
Declaration of interest We have nothing to declare.
Citations
This article has been cited 1 times.- Fakhar-I-Adil M, Angel-Velez D, Araftpoor E, Amin QA, Hedia M, Bühler M, Gevaert K, Menten B, Van Soom A, Chuva de Sousa Lopes SM, Stoop D, De Roo C, Smits K, Heindryckx B. Biphasic CAPA-IVM Improves Equine Oocyte Quality and Subsequent Embryo Development Without Inducing Genetic Aberrations. Int J Mol Sci 2025 Jun 8;26(12).
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