Optimal double-spin method for maximizing the concentration of platelets in equine platelet-rich plasma.
Abstract: This study optimized the double-spin conditions for preparing equine platelet-rich plasma (PRP): leukocyte-rich PRP (L-PRP) and leukocyte-poor PRP (P-PRP). Whole blood samples were centrifuged at various double-spin conditions. Both L-PRP and P-PRP were prepared at each stage, and complete blood counts and growth factor concentrations were compared. Samples centrifuged at 160 × 900 g, 160 × 2,000 g, and 400 × 2,000 g exhibited the highest platelet counts. P-PRP had significantly lower leukocyte and erythrocyte contents than L-PRP, especially at 400 × 2,000 g. No significant differences were observed in growth factor concentrations. Our data suggest that optimum L-PRP preparation should include centrifugation under the aforementioned conditions, whereas centrifugation at 400 × 2,000 g is optimal for P-PRP.
©2020 The Japanese Society of Equine Science.
Publication Date: 2020-12-18 PubMed ID: 33376448PubMed Central: PMC7750641DOI: 10.1294/jes.31.105Google Scholar: Lookup
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- Journal Article
Summary
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The research is about a study that optimizes the preparation method for equine platelet-rich plasma (PRP) with a focus on the double-spin conditions to maximize platelet concentration. The study establishes optimal conditions for Leukocyte-rich (L-PRP) and Leukocyte-poor PRP (P-PRP).
Methodology and Procedures
- The research commenced by taking whole blood samples and centrifuging them under a variety of double-spin conditions.
- Both L-PRP and P-PRP were prepared under each set of conditions.
- Complete blood counts and growth factor concentrations were then compared.
Findings and Observations
- The research found that samples centrifuged at 160 × 900 g, 160 × 2,000 g, and 400 × 2,000 g had the highest platelet counts.
- The study established that P-PRP had significantly lower leukocyte and erythrocyte contents than L-PRP, especially under the condition 400 × 2,000 g.
- No significant differences were observed in regards to the concentrations of growth factors.
Suggestions and Conclusions
- The data collected from the research suggests that the optimal preparation of L-PRP should include centrifugation under the conditions specified in the findings (160 × 900 g, 160 × 2,000 g, and 400 × 2,000 g).
- The study concludes that to optimally prepare P-PRP, centrifugation at 400 × 2,000 g should be employed.
Cite This Article
APA
Fukuda K, Kuwano A, Kuroda T, Tamura N, Mita H, Okada Y, Kasashima Y.
(2020).
Optimal double-spin method for maximizing the concentration of platelets in equine platelet-rich plasma.
J Equine Sci, 31(4), 105-111.
https://doi.org/10.1294/jes.31.105 Publication
Researcher Affiliations
- Clinical Veterinary Medicine Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Clinical Veterinary Medicine Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Clinical Veterinary Medicine Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Clinical Veterinary Medicine Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Clinical Veterinary Medicine Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
- Racehorse Clinic, Miho Training Center, Japan Racing Association, Ibaraki 300-0493, Japan.
- Research Planning and Coordination Division, Equine Research Institute, Japan Racing Association, Tochigi 329-0412, Japan.
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Citations
This article has been cited 2 times.- Reis IL, Lopes B, Sousa P, Sousa AC, Caseiro AR, Mendonça CM, Santos JM, Atayde LM, Alvites RD, Maurício AC. Equine Musculoskeletal Pathologies: Clinical Approaches and Therapeutical Perspectives-A Review. Vet Sci 2024 Apr 26;11(5).
- Zheng JS, Liu SL, Peng XJ, Liu XF, Yu L, Liang SQ. [A prospective study of the effect and mechanism of autologous platelet-rich plasma combined with Meek microskin grafts in repairing the wounds of limbs in severely burned patients]. Zhonghua Shao Shang Za Zhi 2021 Aug 20;37(8):731-737.
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