Optimal frame rate when there were stallion sperm motility evaluations and determinations for kinematic variables using CASA-Mot analysis in different counting chambers.
Abstract: This study was conducted to determine optimum image capture frame rates (FRO) when there was evaluation of different types of counting chambers used for CASA-Mot determinations of stallion sperm motility. Sperm VCL was determined at frame rates of 25-250 f/s in: 1) Spermtrack® (Spk) 10 and 20 chambers (drop displacement-type chambers 10 and 20 μm-deep respectively; and 2) ISAS®D4C10, ISAS®D4C20 (10 and 20 μm-deep respectively) and ISAS®D4C20 L (20 μm-deep) capillary loaded chambers. Values for different sperm kinematic variables were determined using each chamber at 250 f/s, which is the maximum frame rate that the software can be used for analyses. With evaluation of Spk chambers, there was a greater curvilinear velocity (VCL), average path velocity (VAP), straight line velocity (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) values (P < 0.05) than with capillary loaded chambers, with there being greatest values with 20 μm-deep chambers. With the Spk10 chamber, VCL and ALH were greater at the chamber centre than periphery. There were no such differences for the Spk20 chamber. With evaluation of the D4C10 chamber, VSL and STR were less when there was a sperm deposition point towards the chamber end, while there were the opposite for the D4C20 chamber. When there was evaluation of the D4C20 chamber, there were also greater VCL, WOB and BCF values in distal areas. With use of most of these chambers, data should be collected from different fields and means determined, however, this is not necessary with Spk20 chambers.
Copyright © 2020 Elsevier B.V. All rights reserved.
Publication Date: 2020-11-02 PubMed ID: 33157363DOI: 10.1016/j.anireprosci.2020.106643Google Scholar: Lookup
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Summary
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The research conducted focused on determining the best image capture frame rates (FRO) for evaluating the motility of stallion sperm using Computer Assisted Sperm Analysis (CASA-Mot) with various types of counting chambers.
Importance of Optimal Frame Rate in Sperm Analysis
- The study’s objective was to identify the optimal frame rate for capturing images when evaluating the motility of stallion sperm during a CASA-Mot analysis.
- Capturing the movement and behaviour of sperm cells at the right frame rate can provide more accurate assessments of their motility, and therefore, fertility potential.
- Understanding these kinematic variables can offer valuable insights into stallion fertility, leading to more effective breeding strategies.
Different Types of Counting Chambers Used
- The research analyzed the effects of different types of counting chambers: Spermtrack® (10 and 20 μm-deep) and ISAS®D4C10, D4C20 and D4C20 L (10, 20, and 20 μm-deep, respectively).
- These chambers were chosen as they cater to different depths, accommodating variations in sperm cell dimensions.
- Results from the different chamber types were pivotal in addressing the main research question about the optimal frame rate that yields the most accurate analysis results.
Comparing Data from Different Counting Chambers
- Data was collected via a CASA-Mot system from the different chambers at a maximum frame rate of 250 f/s and evaluated.
- Several kinematic variables were considered, such as the curvilinear velocity (VCL), average path velocity (VAP), straight line velocity (STR), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF).
- Based on the results, the Spermtrack® chambers yielded significantly higher values for these variables than the capillary-loaded chambers.
- The highest values were found with the 20 μm-deep Spermtrack® chambers.
Considerations for Optimal Data Collection
- It was noted that for most of the chambers used, data should be collected from different fields with the means determined. However, this was found to be unnecessary with the 20 μm-deep Spermtrack® chambers.
- The study concluded with suggestions of the optimal image capture frame rates and the use of certain types of counting chambers for a more efficient evaluation of stallion sperm motility.
The findings of this study have implications for the field of reproductive biology, particularly in understanding male fertility and potentially improving stallion breeding programs.
Cite This Article
APA
Gacem S, Bompart D, Valverde A, Catalán J, Miró J, Soler C.
(2020).
Optimal frame rate when there were stallion sperm motility evaluations and determinations for kinematic variables using CASA-Mot analysis in different counting chambers.
Anim Reprod Sci, 223, 106643.
https://doi.org/10.1016/j.anireprosci.2020.106643 Publication
Researcher Affiliations
- Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Spain; Proiser R+D, Scientific Park, University of Valencia, C/ Catedràtic Agustín Escardino 9, Building 3 (CUE) Floor 1, 46980 Paterna, Spain.
- Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100 Burjassot, Valencia, Spain; Proiser R+D, Scientific Park, University of Valencia, C/ Catedràtic Agustín Escardino 9, Building 3 (CUE) Floor 1, 46980 Paterna, Spain.
- Proiser R+D, Scientific Park, University of Valencia, C/ Catedràtic Agustín Escardino 9, Building 3 (CUE) Floor 1, 46980 Paterna, Spain; Costa Rica Institute of Technology, School of Agronomy, San Carlos Campus, 223-21001 Alajuela, Costa Rica.
- Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Spain; Proiser R+D, Scientific Park, University of Valencia, C/ Catedràtic Agustín Escardino 9, Building 3 (CUE) Floor 1, 46980 Paterna, Spain.
- Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Spain; Proiser R+D, Scientific Park, University of Valencia, C/ Catedràtic Agustín Escardino 9, Building 3 (CUE) Floor 1, 46980 Paterna, Spain. Electronic address: jordi.miro@uab.cat.
- Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, 46100 Burjassot, Valencia, Spain; Proiser R+D, Scientific Park, University of Valencia, C/ Catedràtic Agustín Escardino 9, Building 3 (CUE) Floor 1, 46980 Paterna, Spain. Electronic address: carles.soler@uv.es.
MeSH Terms
- Animals
- Biomechanical Phenomena
- Horses / physiology
- Image Processing, Computer-Assisted / instrumentation
- Male
- Semen Analysis / veterinary
- Software
- Sperm Count / veterinary
- Sperm Motility / physiology
Citations
This article has been cited 3 times.- Gacem S, Valverde A, Catalán J, Yánez Ortiz I, Soler C, Miró J. A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse. Front Vet Sci 2021;8:651477.
- Brito LFC. Sperm Motility Evaluation in Stallion Fresh, Cooled and Frozen Semen Using a Portable Computer-Assisted Sperm Analysis System. Reprod Domest Anim 2025 Mar;60(3):e70052.
- Hackerova L, Pilsova A, Pilsova Z, Zelenkova N, Tymich Hegrova P, Klusackova B, Chmelikova E, Sedmikova M, Simonik O, Postlerova P. Boar Sperm Motility Assessment Using Computer-Assisted Sperm Analysis: Current Practices, Limitations, and Methodological Challenges. Animals (Basel) 2025 Jan 22;15(3).
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