Optimizing selection of large animals for antibody production by screening immune response to standard vaccines.
Abstract: Antibodies made in large animals are integral to many biomedical research endeavors. Domesticated herd animals like goats, sheep, donkeys, horses and camelids all offer distinct advantages in antibody production. However, their cost of use is often prohibitive, especially where poor antigen response is commonplace; choosing a non-responsive animal can set a research program back or even prevent experiments from moving forward entirely. Over the course of production of antibodies from llamas, we found that some animals consistently produced a higher humoral antibody response than others, even to highly divergent antigens, as well as to their standard vaccines. Based on our initial data, we propose that these "high level responders" could be pre-selected by checking antibody titers against common vaccines given to domestic farm animals. Thus, time and money can be saved by reducing the chances of getting poor responding animals and minimizing the use of superfluous animals.
Copyright © 2016 Elsevier B.V. All rights reserved.
Publication Date: 2016-01-09 PubMed ID: 26775851PubMed Central: PMC4769958DOI: 10.1016/j.jim.2016.01.006Google Scholar: Lookup
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- Journal Article
- Research Support
- N.I.H.
- Extramural
- Research Support
- Non-U.S. Gov't
Summary
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This research focused on improving the selection process for large animals used in the production of antibodies for biomedical research, with the goal of saving resources and improving efficiency in scientific research.
Overview of Animal Selection for Antibody Production
- The study begins by acknowledging the importance of large animals like goats, sheep, donkeys, horses, and camelids, which are often used for producing antibodies for biomedical research.
- However, the research also points out that these animals are expensive to use, and the cost becomes more prohibitive if the animals do not respond well to antigens – substances that trigger immune response.
- One of the challenges in antibody production in these animals is that some animals may not respond well to a specific antigen, which means that the efforts to make these animals produce the needed antibodies may fail, thereby affecting the progress of research activities.
Study Findings
- In this research, there was an observation made while producing antibodies from llamas that some animals consistently produced higher humoral (fluid-based) antibody responses than others, despite differences in the types of antigens introduced.
- The same animals also had a high immune response to the standard vaccines they were given, which posed a potential solution to the previous problem – animals which responded well to their standard vaccines also responded well to other antigens.
Proposal for Optimized Animal Selection
- This observation led the researchers to propose a new method to select animals for antibody production where they would pre-screen animals based on their antibody titers, which are measures of how much antibody an organism produces, against the common vaccines given to domestic farm animals.
- If an animal has high antibody titers in response to a standard vaccine, it is likely that the animal will also have a high immune response to other antigens, making it a good candidate for antibody production.
Benefits of the Proposal
- This approach can save time and money by reducing the risk of selecting an animal with poor response potential, thereby improving the efficiency of lab work.
- It also minimizes the use of unnecessary animals in the research process, contributing to alleviation of ethical concerns in animal-based research.
Cite This Article
APA
Thompson MK, Fridy PC, Keegan S, Chait BT, Fenyö D, Rout MP.
(2016).
Optimizing selection of large animals for antibody production by screening immune response to standard vaccines.
J Immunol Methods, 430, 56-60.
https://doi.org/10.1016/j.jim.2016.01.006 Publication
Researcher Affiliations
- Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY, USA.
- Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY, USA.
- Center for Health Informatics and Bioinformatics, New York University School of Medicine, New York, NY, USA.
- Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, USA.
- Center for Health Informatics and Bioinformatics, New York University School of Medicine, New York, NY, USA.
- Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY, USA. Electronic address: rout@rockefeller.edu.
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Antibody Formation
- Camelids, New World / immunology
- Female
- Green Fluorescent Proteins / immunology
- Luminescent Proteins / immunology
- Male
- Rabies Vaccines / immunology
- Rabies virus / immunology
- Sheep
- Vaccination / veterinary
Grant Funding
- P41 GM103314 / NIGMS NIH HHS
- P41 GM109824 / NIGMS NIH HHS
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Citations
This article has been cited 5 times.- Cross FR, Fridy PC, Ketaren NE, Mast FD, Li S, Olivier JP, Pecani K, Chait BT, Aitchison JD, Rout MP. Expanding and improving nanobody repertoires using a yeast display method: Targeting SARS-CoV-2.. J Biol Chem 2023 Mar;299(3):102954.
- Mast FD, Fridy PC, Ketaren NE, Wang J, Jacobs EY, Olivier JP, Sanyal T, Molloy KR, Schmidt F, Rutkowska M, Weisblum Y, Rich LM, Vanderwall ER, Dambrauskas N, Vigdorovich V, Keegan S, Jiler JB, Stein ME, Olinares PDB, Herlands L, Hatziioannou T, Sather DN, Debley JS, Fenyö D, Sali A, Bieniasz PD, Aitchison JD, Chait BT, Rout MP. Highly synergistic combinations of nanobodies that target SARS-CoV-2 and are resistant to escape.. Elife 2021 Dec 7;10.
- Flores J, Cancino JC, Chavez-Galan L. Lipoarabinomannan as a Point-of-Care Assay for Diagnosis of Tuberculosis: How Far Are We to Use It?. Front Microbiol 2021;12:638047.
- Mast FD, Fridy PC, Ketaren NE, Wang J, Jacobs EY, Olivier JP, Sanyal T, Molloy KR, Schmidt F, Rutkowska M, Weisblum Y, Rich LM, Vanderwall ER, Dambrauskas N, Vigdorovich V, Keegan S, Jiler JB, Stein ME, Olinares PDB, Hatziioannou T, Sather DN, Debley JS, Fenyö D, Sali A, Bieniasz PD, Aitchison JD, Chait BT, Rout MP. Nanobody Repertoires for Exposing Vulnerabilities of SARS-CoV-2.. bioRxiv 2021 Apr 10;.
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