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Theriogenology2008; 70(3); 463-468; doi: 10.1016/j.theriogenology.2008.04.037

Optimizing the use of frozen-thawed equine semen.

Abstract: This manuscript is a review of current protocols, advantages, and disadvantages of breeding mares with frozen-thawed equine semen. Issues affecting pregnancy rates are discussed, including proper mare selection, induction of ovulation, insemination dose, timing of insemination (single-dose versus multiple-dose insemination), methods of insemination (transrectal-guided deep-horn versus hysteroscopic insemination), and post-insemination mare management procedures. In a retrospective analysis of breeding records, a single-dose of frozen-thawed semen was inseminated within 6h post-ovulation; the pregnancy rate (14-16 days after AI) was 67 of 149 (45%). These results were comparable to those previously achieved under commercial conditions, as well as previous studies using multiple doses of frozen-thawed semen per estrous cycle. In conclusion, these data provided evidence that, with appropriate breeding management, an acceptable pregnancy rate can be achieved in mares with a single-dose of frozen-thawed semen (per-cycle) inseminated within 6h after ovulation.
Publication Date: 2008-06-11 PubMed ID: 18550159DOI: 10.1016/j.theriogenology.2008.04.037Google Scholar: Lookup
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Summary

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The research article presents a comprehensive review of the protocols involved in mare breeding using frozen-thawed equine semen, focusing on the factors that affect pregnancy rates. It reveals that with correct breeding management, a satisfactory pregnancy rate can be achieved with a single-dose of frozen-thawed semen inseminated within 6 hours post-ovulation.

Current Protocols, Advantages, and Disadvantages of Breeding Mares with Frozen-Thawed Equine Semen

  • The article dives into the standard methods used in breeding horses, particularly focusing on the practice of using frozen-thawed semen. The authors provide a full assessment, outlining the benefits and drawbacks inherent in this technique.

Factors Impacting Pregnancy Rates

  • The manuscript discusses the variables that can affect a mare’s chances of getting pregnant. These factors include the selection of an appropriate mare, induction of ovulation, dosages, the timing of insemination, the insemination process itself, and post-insemination management procedures for mares.

Insemination Dose and Timing

  • Different approaches were considered concerning the timing and dosage of insemination. Approaches such as single-dose versus multiple-dose insemination were examined.

Insemination Methods

  • The paper analyses varying insemination techniques, such as transrectal-guided deep-horn insemination versus hysteroscopic insemination.

Post-Insemination Mare Management Procedures

  • Finally, the study also emphasizes the significance of managing mares effectively after insemination, a crucial aspect of breeding that directly impacts pregnancy outcomes.

Retrospective Analysis and Results

  • The authors conducted a retrospective analysis of breeding records to further support their discussion, demonstrating that a single dose of frozen-thawed semen could achieve a pregnancy rate of 45% when inseminated within 6 hours post-ovulation.
  • The results were comparable to those achieved under commercial conditions and previous studies involving multiple doses of frozen-thawed semen per estrous cycle, suggesting a level of validity and reliability in the findings.

Conclusion

  • The study concludes that with appropriate breeding management, a satisfactory pregnancy rate can be achieved using a single dose of frozen-thawed semen per cycle, provided it is inseminated within 6 hours after ovulation.

Cite This Article

APA
Miller CD. (2008). Optimizing the use of frozen-thawed equine semen. Theriogenology, 70(3), 463-468. https://doi.org/10.1016/j.theriogenology.2008.04.037

Publication

ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 70
Issue: 3
Pages: 463-468

Researcher Affiliations

Miller, C D
  • Equine Medical Center of Ocala, 7107 West Highway 326, Ocala, FL 34482, USA. CMi047690@aol.com

MeSH Terms

  • Animals
  • Freezing
  • Horses / physiology
  • Male
  • Semen / physiology
  • Semen Preservation / methods
  • Spermatozoa / cytology
  • Spermatozoa / physiology

References

This article includes 20 references

Citations

This article has been cited 7 times.
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  2. de Oliveira RA, Alonso MA, Fonte JS, Fernandes CB. Equine ICSI: an update on semen perspective. Anim Reprod 2024;21(4):e20240015.
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    doi: 10.3390/ani14091264pubmed: 38731267google scholar: lookup
  4. Seyedasgari F, Asadi B, Kim E. Seminal plasma modulates post-thaw longevity and motility of frozen sperm in dromedary camel. Anim Biosci 2023 Dec;36(12):1821-1830.
    doi: 10.5713/ab.23.0136pubmed: 37641835google scholar: lookup
  5. Colombo M, Morselli MG, Franchi G, Schäfer-Somi S, Luvoni GC. Freezability of Dog Semen after Collection in Field Conditions and Cooled Transport. Animals (Basel) 2022 Mar 23;12(7).
    doi: 10.3390/ani12070816pubmed: 35405806google scholar: lookup
  6. Gmel AI, Burger D, Neuditschko M. A Novel QTL and a Candidate Gene Are Associated with the Progressive Motility of Franches-Montagnes Stallion Spermatozoa after Thaw. Genes (Basel) 2021 Sep 25;12(10).
    doi: 10.3390/genes12101501pubmed: 34680896google scholar: lookup
  7. Hannan MA, Haneda S, Murata K, Takeuchi S, Cheong SH, Nambo Y. Birth of first foals through embryo transfer after artificial insemination using frozen semen in Japan. J Reprod Dev 2020 Apr 10;66(2):193-197.
    doi: 10.1262/jrd.2019-117pubmed: 31983706google scholar: lookup