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Veterinary research communications2011; 35(7); 463-468; doi: 10.1007/s11259-011-9485-7

Optimum conditions for serum clearance of iodixanol, applicable to the estimation of glomerular filtration rate in horses.

Abstract: To estimate the glomerular filtration rate (GFR) in horses, an optimum dose of the nonionic contrast medium iodixanol as a tracer was assessed with blood-sample times. Iodixanol was administered intravenously at 10-40 mg I/kg to geldings and mares, and blood was collected 30, 60, 90, 120, 150, and 180 min later. Serum iodixanol concentration was determined by high-performance liquid chromatography (HPLC), and serum urea nitrogen (UN) and creatinine concentrations were also measured. The combination of 20 mg I/kg iodixanol and sampling times of 60, 90, and 120 min after injection was considered to be appropriate for practical use. In clinically healthy horses, the reference values were determined to be 1.90 ± 0.03 ml/min/kg (150.8 ± 2.94 ml/min/m2), consistent with historical data using different tracers. The result suggests that serum clearance of iodixanol is a ready-to-use tool for a screening of alterations in the equine GFR, although it is necessary to perform a more longitudinal study using horses with a variety of renal functions.
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Publication Date: 2011-05-25 PubMed ID: 21611831DOI: 10.1007/s11259-011-9485-7Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the optimal conditions for using iodixanol, a contrast medium, to estimate the glomerular filtration rate (GFR) in horses. It found that a 20 mg I/kg dose and specific sampling times were appropriate, providing a tool for screening changes in equine GFR.

Study Methodology

  • The study began by administering iodixanol intravenously to both geldings and mares at doses ranging from 10 to 40 mg I/kg.
  • Blood samples were then collected at multiple time points ranging from 30 to 180 minutes after injection. This was done to assess how the iodixanol concentration in the horse’s blood changed over time, and thus understand the rate of the contrast medium clearance from the blood.
  • The serum iodixanol concentration was determined via high-performance liquid chromatography (HPLC), providing precise measurements of the iodixanol levels in the blood at each timepoint.
  • Concentrations of serum urea nitrogen (UN) and creatinine were also measured as these are commonly used indicators of kidney function, against which the findings could be compared.

Key Findings

  • The research found that a combination of a 20 mg I/kg dose of iodixanol and blood sampling times of 60, 90, and 120 minutes post-injection was the best approach for practical use.
  • In healthy horses, reference values for GFR were found to be 1.90 ± 0.03 ml/min/kg (150.8 ± 2.94 ml/min/m2). These values align with earlier data obtained using different tracers, thus validating the proposed method.

Implications of the Study

  • One of the primary outcomes of the research is it suggests that serum clearance of iodixanol could be a viable tool for monitoring changes in glomerular filtration rate in horses, and by extension, their kidney function.
  • However, the authors emphasize that further longitudinal studies are essential, which should involve horses with varying renal functions. This would allow a more thorough evaluation of the technique’s efficacy across a broader range of scenarios, further cementing its clinical utility.

Cite This Article

APA
Satoh H, Abe S, Kato M, Saito J, Furuhama K. (2011). Optimum conditions for serum clearance of iodixanol, applicable to the estimation of glomerular filtration rate in horses. Vet Res Commun, 35(7), 463-468. https://doi.org/10.1007/s11259-011-9485-7

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 35
Issue: 7
Pages: 463-468

Researcher Affiliations

Satoh, Hiroshi
  • Shinwa BioCraft Laboratory Inc., 1-24-50 Edogawa, Edogawa-ku, Tokyo, 132-0013, Japan.
Abe, Sumito
    Kato, Masaki
      Saito, Jun
        Furuhama, Kazuhisa

          MeSH Terms

          • Animals
          • Chromatography, High Pressure Liquid / veterinary
          • Contrast Media / administration & dosage
          • Contrast Media / analysis
          • Contrast Media / pharmacokinetics
          • Female
          • Glomerular Filtration Rate / veterinary
          • Horses
          • Kidney Diseases / diagnosis
          • Kidney Diseases / veterinary
          • Male
          • Time Factors
          • Triiodobenzoic Acids / administration & dosage
          • Triiodobenzoic Acids / blood
          • Triiodobenzoic Acids / pharmacokinetics

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          Citations

          This article has been cited 1 times.
          1. Miyaki T, Xu J, Sugiura M, Kaneda H, Trejo JAO, Kawasaki Y, Omotehara T, Negishi-Koga T, Ishijima M, Yamaguchi J, Kakuta S, Ichimura K. Primary cilium disappearance in podocytes during vertebrate phylogeny revealed by array tomography. Cell Tissue Res 2025 Oct;402(1):51-63.
            doi: 10.1007/s00441-025-04002-zpubmed: 40877611google scholar: lookup
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