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Home / Equine Research Bank / Int J Mol Sci / Orientin Reverses Premature Senescence in Equine Adipose Str...
International journal of molecular sciences2025; 26(14); 6867; doi: 10.3390/ijms26146867

Orientin Reverses Premature Senescence in Equine Adipose Stromal Cells Affected by Equine Metabolic Syndrome Through Oxidative Stress Modulation.

Abstract: Equine metabolic syndrome (EMS) is a prevalent endocrine disorder associated with insulin dysregulation, oxidative stress, and impaired regenerative capacity of adipose-derived stem cells (ASCs). The aim of this study was to evaluate the effects of orientin-a plant-derived flavonoid with known antioxidant properties-on equine ASCs (EqASCs) derived from both clinically healthy and diagnosed EMS-affected mares. EqASCs were treated with orientin to evaluate its biological effects. The analysis included key cellular functions such as proliferative capacity, viability, apoptosis, oxidative stress, senescence, clonogenicity, and migration. Orientin significantly enhanced the proliferative activity of EqASCs, as evidenced by increased Ki67 expression and favorable alterations in cell cycle distribution. In addition, the treatment improved overall cell viability, reduced apoptotic activity, and restored both the clonogenic potential and migratory capacity of the cells, with particularly pronounced effects observed in EqASCs isolated from EMS-affected horses. Importantly, orientin also led to a marked reduction in cellular senescence and oxidative stress, further suggesting its potential as a protective and regenerative agent in metabolically impaired ASCs. These findings indicate that orientin can exert comprehensive cytoprotective effects on EqASCs, with pronounced benefits in cells derived from EMS-affected animals. By improving multiple functional parameters, orientin emerges as a promising candidate for therapeutic strategies aimed at restoring the regenerative potential of ASCs compromised by metabolic dysregulation in horses.
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Publication Date: 2025-07-17 PubMed ID: 40725115PubMed Central: PMC12295333DOI: 10.3390/ijms26146867Google Scholar: Lookup
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APA
Orzoł D, Kępska M, Zyzak M. (2025). Orientin Reverses Premature Senescence in Equine Adipose Stromal Cells Affected by Equine Metabolic Syndrome Through Oxidative Stress Modulation. Int J Mol Sci, 26(14), 6867. https://doi.org/10.3390/ijms26146867

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 26
Issue: 14
PII: 6867

Researcher Affiliations

Orzoł, Dominika
  • Department of Experimental Biology, Institute of Biology, Wrocław University of Environmental and Life Sciences, C. K. Norwida 25, 50-375 Wrocław, Poland.
Kępska, Martyna
  • Department of Experimental Biology, Institute of Biology, Wrocław University of Environmental and Life Sciences, C. K. Norwida 25, 50-375 Wrocław, Poland.
Zyzak, Magdalena
  • Department of Experimental Biology, Institute of Biology, Wrocław University of Environmental and Life Sciences, C. K. Norwida 25, 50-375 Wrocław, Poland.

MeSH Terms

  • Animals
  • Horses
  • Oxidative Stress / drug effects
  • Metabolic Syndrome / metabolism
  • Metabolic Syndrome / veterinary
  • Metabolic Syndrome / drug therapy
  • Metabolic Syndrome / pathology
  • Flavonoids / pharmacology
  • Cellular Senescence / drug effects
  • Adipose Tissue / cytology
  • Adipose Tissue / metabolism
  • Glucosides / pharmacology
  • Cell Survival / drug effects
  • Cell Proliferation / drug effects
  • Female
  • Apoptosis / drug effects
  • Horse Diseases / metabolism
  • Horse Diseases / drug therapy
  • Horse Diseases / pathology
  • Stromal Cells / drug effects
  • Stromal Cells / metabolism
  • Cell Movement / drug effects
  • Cells, Cultured

Grant Funding

  • N010/0011/24 / Wrocu0142aw University of Environmental and Life Sciences

Conflict of Interest Statement

The authors declare no conflicts of interest.

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