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Home / Equine Research Bank / Vet Res Commun / Osteoinductivity of gelatin/β-tricalcium phosphate spo...
Veterinary research communications2014; 38(1); 73-80; doi: 10.1007/s11259-013-9587-5

Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect model.

Abstract: Fracture is one of the most life-threatening injuries in horses. Fracture repair is often associated with unsatisfactory outcomes and is associated with a high incidence of complications. This study aimed to evaluate the osteogenic effects of gelatin/β-tricalcium phosphate (GT) sponges loaded with different concentrations/ratios of mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) in an equine bone defect model. Seven thoroughbred horses were used in this study. Eight bone defects were created in the third metatarsal bones of each horse. Then, eight treatments, namely control, GT, GT/M-5, GT/M-6, GT/M-5/B-1, GT/M-5/B-3, GT/M-6/B-1, and GT/M-6/B-3 were applied to the eight different sites in a randomized manner (M-5: 2 × 10(5) MSCs; M-6: 2 × 10(6) MSCs; B-1: 1 μg of BMP-2; B-3: 3 μg of BMP-2). Repair of bone defects was assessed by radiography, quantitative computed tomography (QCT), and histopathological evaluation. Radiographic scores and CT values were significantly lower in the control group than in the other groups, while they were significantly higher in the GT/M-5/B-3 and GT/M-6/B-3 groups than in the other groups. The amount of mature compact bone filling the defects was greater in the GT/M-5/B-3 and GT/M-6/B-3 groups than in the other groups. The present study demonstrated that the GT sponge loaded with MSCs and BMP-2 promoted bone regeneration in an equine bone defect model. The GT/MSC/BMP-2 described here may be useful for treating horses with bone injuries.
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Publication Date: 2014-01-18 PubMed ID: 24442646DOI: 10.1007/s11259-013-9587-5Google Scholar: Lookup
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Summary

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This study examines the effects of using gelatin/β-tricalcium phosphate sponges containing varying quantities of mesenchymal stem cells and bone morphogenetic protein-2 on bone regeneration in horses. It finds that these sponges significantly promote bone regeneration, offering promise for treating equine bone injuries.

Study Aim and Procedure

  • The main aim of this study was to examine the effectiveness of gelatin/β-tricalcium phosphate (GT) sponges, infused with different concentrations of mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2), in promoting bone regeneration using an equine bone defect model.
  • Seven thoroughbred horses were used for the study, where eight bone defects each were created intentionally in the third metatarsal bones of the horses. Following this, eight different treatment conditions were randomly applied to these defect sites, consisting various combinations of GT, MSCs and BMP-2.
  • The improvement in bone defects was analyzed using radiography, quantitative computed tomography (QCT), and histopathological evaluations.

Key Results

  • According to the findings of the assessment tools (radiographic scores and CT values), the control group (having no treatment) showed the lowest scores.
  • On the other hand, treatment groups with GT/M-5/B-3 (2×10(5) MSCs and 3μg of BMP-2) and GT/M-6/B-3 (2×10(6) MSCs and 3μg of BMP-2) showed significantly higher scores compared to all the other group combinations, suggesting these as the most effective combinations for bone regeneration.
  • A higher amount of mature compact bone was observed filling the defects in the GT/M-5/B-3 and GT/M-6/B-3 groups than other groups, providing further validation for these combinations’ effectiveness.

Significance and Utility of Findings

  • This study showcased that gelatin/β-tricalcium phosphate sponges loaded with MSCs and BMP-2 could efficiently promote bone regeneration in horses.
  • The specific combinations of GT/M-5/B-3 and GT/M-6/B-3 provided the best results, making them promising treatments for bone injuries in the equine population.

Cite This Article

APA
Seo JP, Tsuzuki N, Haneda S, Yamada K, Furuoka H, Tabata Y, Sasaki N. (2014). Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect model. Vet Res Commun, 38(1), 73-80. https://doi.org/10.1007/s11259-013-9587-5

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 38
Issue: 1
Pages: 73-80

Researcher Affiliations

Seo, Jong-Pil
  • Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro-City, Hokkaido, 080-8555, Japan.
Tsuzuki, Nao
    Haneda, Shingo
      Yamada, Kazutaka
        Furuoka, Hidefumi
          Tabata, Yasuhiko
            Sasaki, Naoki

              MeSH Terms

              • Animals
              • Bone Morphogenetic Protein 2 / pharmacology
              • Bone Regeneration / drug effects
              • Bone and Bones / drug effects
              • Bone and Bones / injuries
              • Calcium Phosphates / pharmacology
              • Female
              • Fractures, Bone / therapy
              • Fractures, Bone / veterinary
              • Gelatin Sponge, Absorbable / pharmacology
              • Gelatin Sponge, Absorbable / therapeutic use
              • Horses
              • Male
              • Mesenchymal Stem Cells / cytology
              • Osteogenesis / drug effects
              • Random Allocation
              • Treatment Outcome

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              Citations

              This article has been cited 13 times.
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