Osteoprogenitor cell therapy in an equine fracture model.
Abstract: To compare the efficacy of osteoprogenitors in fibrin glue to fibrin glue alone in bone healing of surgically induced ostectomies of the fourth metacarpal bones in an equine model. Methods: Experimental. Methods: Adult horses (n = 10). Methods: Segmental ostectomies of the 4th metacarpal bone (MC4) were performed bilaterally in 10 horses. There was 1 treatment and 1 control limb in each horse. Bone defects were randomly injected with either fibrin glue and osteoprogenitor cells or fibrin glue alone. Radiography was performed every week until the study endpoint at 12 weeks. After euthanasia, bone healing was evaluated using radiography and histology. Analysis of radiographic data was conducted using a linear-mixed model. Analysis of histologic data was conducted using a general linear model. Statistical significance was set at P < .05. Results: Radiographic grayscale data as a measure of bone healing revealed no significant difference between treatment and control limbs. Radiographic scoring results also showed that the treatment effect was not significant. Histologic analysis was consistent with radiographic analysis showing no significant difference between the area of bone present in treatment and control limbs. Conclusions: Injection of periosteal-derived osteoprogenitors in a fibrin glue carrier into surgically created ostectomies of MC4 does not accelerate bone healing when compared with fibrin glue alone.
© Copyright 2012 by The American College of Veterinary Surgeons.
Publication Date: 2012-07-13 PubMed ID: 22804243DOI: 10.1111/j.1532-950X.2012.01024.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigated the potential for osteoprogenitor cells, which help form new bone, mixed with fibrin glue to aid in the healing of bone fractures in horses. Unfortunately, the study found that there was no noticeable improvement in bone healing when compared to using fibrin glue alone.
Study Design
- This was an experimental study conducted on ten adult horses, each of which underwent bilateral segmental ostectomies – a surgical procedure that involves the removal of sections of bone – of the fourth metacarpal bone (MC4).
- For each horse, one limb was treated with a combination of osteoprogenitor cells and fibrin glue while the other limb, serving as control, was treated with fibrin glue alone. This setup allowed the researchers to directly compare the effects of the two treatments.
- Progress of bone healing was monitored using radiography every week, and the study spanned a total of 12 weeks.
Results Analysis
- On week 12, the horses were euthanized and the healing of the bone was evaluated using both radiography and histology – the study of microscopic anatomy.
- Two statistical models were applied for the analysis: a linear-mixed model for radiographic data and a general linear model for histologic data.
- Radiography and histology results did not show significant differences in bone healing between the treatment and control limbs, both according to grayscale data as a measure of bone healing and radiographic scoring results.
Conclusion
- From the results of this study, it was concluded that injecting periosteal-derived osteoprogenitors – cells from the outer surface of bone that can differentiate into bone-forming cells – mixed with fibrin glue into surgically-created bone defects did not accelerate bone healing when compared with fibrin glue alone.
- This finding suggests that the standalone application of fibrin glue is sufficient for aiding bone recovery after an ostectomy, and that adding osteoprogenitor cells does not confer additional benefit.
Cite This Article
APA
McD￮ LA, Pack L, Lores M, Wright GM, Esparza-Gonzalez B, Masaoud E.
(2012).
Osteoprogenitor cell therapy in an equine fracture model.
Vet Surg, 41(7), 773-783.
https://doi.org/10.1111/j.1532-950X.2012.01024.x Publication
Researcher Affiliations
- Department of Health Management, University of Prince Edward Island, Charlottetown, PEI, Canada. lmcd￮@upei.ca
MeSH Terms
- Animals
- Disease Models, Animal
- Fibrin Tissue Adhesive / therapeutic use
- Fracture Healing / physiology
- Fractures, Bone / therapy
- Fractures, Bone / veterinary
- Fractures, Ununited / veterinary
- Horses / injuries
- Metacarpal Bones / injuries
- Metacarpal Bones / physiology
- Osteogenesis / physiology
- Periosteum / cytology
- Random Allocation
- Stem Cell Transplantation / methods
- Stem Cell Transplantation / veterinary
- Stem Cells / cytology
- Stem Cells / physiology
Grant Funding
- Canadian Institutes of Health Research
Citations
This article has been cited 7 times.- Grzeskowiak RM, Alghazali KM, Hecht S, Donnell RL, Doherty TJ, Smith CK, Anderson DE, Biris AS, Adair HS. Influence of a novel scaffold composed of polyurethane, hydroxyapatite, and decellularized bone particles on the healing of fourth metacarpal defects in mares.. Vet Surg 2021 Jul;50(5):1117-1127.
- Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases.. Animals (Basel) 2021 Jan 19;11(1).
- Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do.. Front Bioeng Biotechnol 2020;8:972.
- Gugjoo MB, Hussain S, Amarpal, Shah RA, Dhama K. Mesenchymal Stem Cell-Mediated Immuno-Modulatory and Anti- Inflammatory Mechanisms in Immune and Allergic Disorders.. Recent Pat Inflamm Allergy Drug Discov 2020;14(1):3-14.
- Al Naem M, Bourebaba L, Kucharczyk K, Röcken M, Marycz K. Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders.. Stem Cell Rev Rep 2020 Apr;16(2):301-322.
- Pfeiffenberger M, Bartsch J, Hoff P, Ponomarev I, Barnewitz D, Thöne-Reineke C, Buttgereit F, Gaber T, Lang A. Hypoxia and mesenchymal stromal cells as key drivers of initial fracture healing in an equine in vitro fracture hematoma model.. PLoS One 2019;14(4):e0214276.
- Seo JP, Tsuzuki N, Haneda S, Yamada K, Furuoka H, Tabata Y, Sasaki N. Osteoinductivity of gelatin/β-tricalcium phosphate sponges loaded with different concentrations of mesenchymal stem cells and bone morphogenetic protein-2 in an equine bone defect model.. Vet Res Commun 2014 Mar;38(1):73-80.
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