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Home / Equine Research Bank / Transbound Emerg Dis / Outbreak of African horse sickness in Thailand, 2020.
Transboundary and emerging diseases2020; 67(5); 1764-1767; doi: 10.1111/tbed.13701

Outbreak of African horse sickness in Thailand, 2020.

Abstract: African horse sickness was confirmed in horses in Thailand during March 2020. The virus was determined to belong to serotype 1 and is phylogenetically closely related to isolates from South Africa. This is the first incidence of African horse sickness occurring in South East Asia and of serotype 1 outside of Africa.
© 2020 The Authors. Transboundary and Emerging Diseases published by Blackwell Verlag GmbH.
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Publication Date: 2020-07-15 PubMed ID: 32593205DOI: 10.1111/tbed.13701Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article elaborates on the outbreak of African horse sickness, a severe and often fatal disease in horses, in Thailand in March 2020. The identified virus was found to be closely related to isolates from South Africa, and it marked the first instance of this disease occurring in South East Asia, and the first occurrence of the serotype 1 outside of Africa.

Context and Significance of the Research

  • This research is significant because it identifies and records the first outbreak of African horse sickness (AHS) in Thailand, and by extension, in South East Asia.
  • It further signifies the first detection of the serotype 1 of the AHS virus outside Africa, linking it to be closely related to isolates from South Africa.
  • African horse sickness is a highly infectious and lethal disease that could decimate horse populations and severely affect related industries and livelihoods. Thus, the study of its propagation presents a critical subject in veterinary science and public health.

Key Findings of the Research

  • The researchers concluded that the AHS virus detected in horses in Thailand in March 2020 belongs to serotype 1, marking an extension of the disease beyond the usual geographic constraints (Africa).
  • Through phylogenetic analysis, the virus was found to be closely related to isolates from South Africa, suggesting sources or transmission routes of the disease.

Implications of the Research

  • The identification of AHS and its serotype in Thailand could lead to better prevention and control strategies for the disease. This could include improved vaccines developed for this specific serotype and educational programs for the local horse industry on how to prevent the spread of the disease.
  • The research could be significant for disease surveillance systems, helping the authorities manage outbreaks better via early detection and response strategies.
  • The finding that the AHS virus in Thailand is closely related to isolates from South Africa could help trace the origins and spread of the disease, providing vital information for controlling and preventing future outbreaks.

Cite This Article

APA
King S, Rajko-Nenow P, Ashby M, Frost L, Carpenter S, Batten C. (2020). Outbreak of African horse sickness in Thailand, 2020. Transbound Emerg Dis, 67(5), 1764-1767. https://doi.org/10.1111/tbed.13701

Publication

Transboundary and emerging diseases
ISSN: 1865-1682
NlmUniqueID: 101319538
Country: Germany
Language: English
Volume: 67
Issue: 5
Pages: 1764-1767

Researcher Affiliations

King, Simon
  • The Pirbright Institute, Pirbright, UK.
Rajko-Nenow, Paulina
  • The Pirbright Institute, Pirbright, UK.
Ashby, Martin
  • The Pirbright Institute, Pirbright, UK.
Frost, Lorraine
  • The Pirbright Institute, Pirbright, UK.
Carpenter, Simon
  • The Pirbright Institute, Pirbright, UK.
Batten, Carrie
  • The Pirbright Institute, Pirbright, UK.

Grant Funding

  • BBS/E/I/00007033 / Biotechnology and Biological Sciences Research Council
  • BBS/E/I/00007036 / Biotechnology and Biological Sciences Research Council
  • BBS/E/I/00007037 / Biotechnology and Biological Sciences Research Council

References

This article includes 15 references
  1. Aguero M, Gomez-Tejedor C, Angeles Cubillo M, Rubio C, Romero E, Jimenez-Clavero A. Real-time fluorogenic reverse transcription polymerase chain reaction assay for detection of African horse sickness virus. Journal of Veterinary Diagnostic Investigation 20(3), 325-328.
    doi: 10.1177/104063870802000310google scholar: lookup
  2. Bachanek-Bankowska K, Maan S, Castillo-Olivares J, Manning NM, Maan NS, Potgieter AC, Mertens PPC. Real time RT-PCR assays for detection and typing of African horse sickness virus. PLoS One 9(4), e93758.
    doi: 10.1371/journal.pone.0093758google scholar: lookup
  3. Carpenter S, Mellor PS, Fall AG, Garros C, Venter GJ. African Horse Sickness Virus: History, Transmission, and Current Status. Annual Review of Entomology 62, 343-358.
    doi: 10.1146/annurev-ento-031616-035010google scholar: lookup
  4. Dennis SJ, Meyers AE, Hitzeroth II, Rybicki EP. African horse sickness: A review of current understanding and vaccine. Development. Viruses 11(9), 844.
    doi: 10.3390/v11090844google scholar: lookup
  5. FAO (2020). FAOSTAT. Retrieved from http://www.fao.org/faostat/en/#data/QA/visualize
  6. Guthrie AJ, MacLachlan NJ, Joone C, Lourens CW, Weyer CT, Quan M, Gardner IA. Diagnostic accuracy of a duplex real-time reverse transcription quantitative PCR assay for detection of African horse sickness virus. Journal of Virological Methods 189(1), 30-35.
    doi: 10.1016/j.jviromet.2012.12.014google scholar: lookup
  7. Howell PG. The 1960 epizootic of African Horsesickness in the Middle East and S.W. Asia. Journal of the South African Veterinary Association 31(3), 329-334.
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33(7), 1870-1874.
    doi: 10.1093/molbev/msw054google scholar: lookup
  9. Lu G, Pan J, Ou J, Shao R, Hu X, Wang C, Li S. African horse sickness: Its emergence in Thailand and potential threat to other Asian countries. Transboundary and Emerging Diseases [Epub ahead of print].
    doi: 10.1111/tbed.13625google scholar: lookup
  10. Rajko-Nenow P, Christodoulou V, Thurston W, Ropiak HM, Savva S, Brown H, Batten C. Origin of bluetongue virus serotype 8 outbreak in cyprus, September 2016. Viruses 12(1), 96.
    doi: 10.3390/v12010096google scholar: lookup
  11. Robin M, Page P, Archer D, Baylis M. African horse sickness: The potential for an outbreak in disease-free regions and current disease control and elimination techniques. Equine Veterinary Journal 48(5), 659-669.
    doi: 10.1111/evj.12600google scholar: lookup
  12. Rodriguez M, Hooghuis H, Castano M. African horse sickness in Spain. Veterinary Microbiology 33(1-4), 129-142.
    doi: 10.1016/0378-1135(92)90041-qgoogle scholar: lookup
  13. Thepparat A, Bellis G, Ketavan C, Ruangsittichai J, Sumruayphol S, Apiwathnasorn C. Ten species of Culicoides Latreille (Diptera: Ceratopogonidae) newly recorded from Thailand. Zootaxa 4033(1), 48-56.
    doi: 10.11646/zootaxa.4033.1.2google scholar: lookup
  14. Weyer CT, Joone C, Lourens CW, Monyai MS, Koekemoer O, Grewar JD, Guthrie AJ. Development of three triplex real-time reverse transcription PCR assays for the qualitative molecular typing of the nine serotypes of African horse sickness virus. Journal of Virological Methods 223, 69-74.
    doi: 10.1016/j.jviromet.2015.07.015google scholar: lookup
  15. Wirth WW, Hubert AA. The Culicoides of Southeast Asia (Diptera:Ceratopogonidae). Memoirs of the American Entomological Institute 44, 1-508.

Citations

This article has been cited 39 times.
  1. Penzhorn L, Crafford JE, Guthrie AJ. Enhancing African horse sickness virus detection: comparing and adapting PCR assays. J Vet Diagn Invest 2026 Feb 7;:10406387261417355.
    doi: 10.1177/10406387261417355pubmed: 41653013google scholar: lookup
  2. Ma X, Zhang M, Zhang X, Qi T, Zhang W, Zhao Y, Na L, Zhang Y, Wang XF, Wang X. Construction and Immunogenicity Evaluation of a Recombinant Fowlpox Virus Expressing VP2 Gene of African Horse Sickness Virus Serotype 1. Microorganisms 2025 Dec 9;13(12).
    doi: 10.3390/microorganisms13122807pubmed: 41472010google scholar: lookup
  3. Morales J, Ruano MJ, Tena-Tomás C, van Schalkwyk A, Loundras EA, Valero-Lorenzo M, López-Herranz A, Romito M, Batten C, Villalba R, Agüero M. Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. Microorganisms 2025 Nov 25;13(12).
    doi: 10.3390/microorganisms13122684pubmed: 41471886google scholar: lookup
  4. White J, Thompson K, van den Berg D, O'Neill G, Mendez DH, Talwar J, Degeling C, Forsythe R, Durrheim DN. 'Pretty devastating': exploring horse owner and veterinarian lived experiences of the equine Hendra virus. Front Vet Sci 2025;12:1661615.
    doi: 10.3389/fvets.2025.1661615pubmed: 41357753google scholar: lookup
  5. Zhang M, Wang XF, Guo SF, Wang L, Fu BF, Wang JW, Song YF, Yang XY, Hao SY, Zhang QY, Zhang B, Yang CH. Identification and Genetic Characterization of a Strain of African Horse Sickness Virus Serotype 1 and Its Safety Evaluation in a Mouse Model. Microorganisms 2025 Oct 6;13(10).
    doi: 10.3390/microorganisms13102314pubmed: 41156774google scholar: lookup
  6. Pipitpornsirikul P, Thangthamniyom N, Laikul A, Songkasupa T, Pathomsakulwong W, Apichaimongkonkun T, Kasemsuwan S, E-Kobon T, Lekcharoensuk P. The Viremic Phase and Humoral Immune Response Against African Horse Sickness Virus That Emerged in Thailand in 2020. Vet Sci 2025 Sep 11;12(9).
    doi: 10.3390/vetsci12090878pubmed: 41012803google scholar: lookup
  7. Tinarwo M, Dennis SJ, Hitzeroth II, Meyers AE, Rybicki EP, Mbewana S. Development of an African horse sickness VP6 DIVA diagnostic ELISA. Virol J 2025 Aug 12;22(1):276.
    doi: 10.1186/s12985-025-02898-1pubmed: 40796889google scholar: lookup
  8. Kerdput V, Yodkamol V, Sookprasong M, Wongwadhunyoo W, Khunsri I, Masrinoul P, Wisedchanwet T, Uthailak N, Limpanont Y, Reamtong O, Adisakwattana P, Thawornkuno C. Production and evaluation of rabies immunoglobulin extracted from chicken egg yolk. Biotechnol Rep (Amst) 2025 Jun;46:e00897.
    doi: 10.1016/j.btre.2025.e00897pubmed: 40487875google scholar: lookup
  9. Punyadarsaniya D, Taesuji M, Rattanamas K, Ruenphet S. Establishment of an In-House Indirect Enzyme-Linked Immunosorbent Assay to Detect Antibodies Against African Horse Sickness Based on Monovalent and Polyvalent Live Attenuated Vaccines During the First Outbreak in Thailand. Animals (Basel) 2025 May 15;15(10).
    doi: 10.3390/ani15101433pubmed: 40427310google scholar: lookup
  10. Archana M, Nayankumar, Sundarraj R, Mruthyunjaya AG, Ghosal T, Mazumdar A, Hemadri D, Sengupta PP, Prasad M, Reddy YN, Yarabolu KR, Ummer J, Misri J, Rahman H, Shome BR, Shivachandra SB, Chanda MM. Abundance and Diversity of Culicoides Species (Diptera: Ceratopogonidae) in Different Forest Landscapes of Karnataka, India: Implications for Culicoides Borne Diseases. Transbound Emerg Dis 2023;2023:6250963.
    doi: 10.1155/2023/6250963pubmed: 40303748google scholar: lookup
  11. Huang C, Wang J, Ruan Z, Wu J, Lin Y, Cao C, Yang J, Weng Q, Jin Y, Chen P, Hua Q. Real-Time Reverse Transcription Multienzyme Isothermal Rapid Amplification for Rapid Detection of African Horse Sickness Virus. Transbound Emerg Dis 2025;2025:1852368.
    doi: 10.1155/tbed/1852368pubmed: 40302746google scholar: lookup
  12. Li ZH, Wang YN, Deng JM, Li L, Yang LJ, Chen XQ, Wang WH, Lu FY, Tang ZJ, Wang DM, Duan YL. Searching for potential Culicoides vectors of four orbiviruses in Yunnan Province, China. Parasit Vectors 2025 Feb 24;18(1):73.
    doi: 10.1186/s13071-025-06679-1pubmed: 39994809google scholar: lookup
  13. Thepparat A, Kamata N, Siriyasatien P, Prempree W, Dasuntad K, Chittsamart B, Sanguansub S. Seasonal Abundance and Diversity of Culicoides Biting Midges in Livestock Sheds in Kanchanaburi Province, Thailand. Insects 2024 Sep 14;15(9).
    doi: 10.3390/insects15090701pubmed: 39336668google scholar: lookup
  14. Ashby M, Moore R, King S, Newbrook K, Flannery J, Batten C. Designing a Multiplex PCR-xMAP Assay for the Detection and Differentiation of African Horse Sickness Virus, Serotypes 1-9. Microorganisms 2024 May 3;12(5).
    doi: 10.3390/microorganisms12050932pubmed: 38792762google scholar: lookup
  15. Taesuji M, Rattanamas K, Yim PB, Ruenphet S. Stability and Detection Limit of Avian Influenza, Newcastle Disease Virus, and African Horse Sickness Virus on Flinders Technology Associates Card by Conventional Polymerase Chain Reaction. Animals (Basel) 2024 Apr 21;14(8).
    doi: 10.3390/ani14081242pubmed: 38672390google scholar: lookup
  16. Villalba R, Tena-Tomás C, Ruano MJ, Valero-Lorenzo M, López-Herranz A, Cano-Gómez C, Agüero M. Development and Validation of Three Triplex Real-Time RT-PCR Assays for Typing African Horse Sickness Virus: Utility for Disease Control and Other Laboratory Applications. Viruses 2024 Mar 20;16(3).
    doi: 10.3390/v16030470pubmed: 38543834google scholar: lookup
  17. Poochipakorn C, Joongpan W, Tongsangiam P, Phooseerit A, Leelahapongsathon K, Chanda M. The impact of strategic ventilation adjustments on stress responses in horses housed full-time in a vector-protected barn during the African horse sickness outbreak in Thailand. Anim Welf 2023;32:e19.
    doi: 10.1017/awf.2023.10pubmed: 38487428google scholar: lookup
  18. Li N, Meng J, He Y, Wang W, Wang J. Potential roles of Culicoides spp. (Culicoides imicola, Culicoides oxystoma) as biological vectors of bluetongue virus in Yuanyang of Yunnan, P. R. China. Front Cell Infect Microbiol 2023;13:1283216.
    doi: 10.3389/fcimb.2023.1283216pubmed: 38274733google scholar: lookup
  19. Kunanusont N, Taesuji M, Kulthonggate U, Rattanamas K, Mamom T, Thongsri K, Phannithi T, Ruenphet S. Longitudinal humoral immune response and maternal immunity in horses after a single live-attenuated vaccination against African horse sickness during the disease outbreak in Thailand. Vet World 2023 Aug;16(8):1690-1694.
    doi: 10.14202/vetworld.2023.1690-1694pubmed: 37766699google scholar: lookup
  20. Kamyingkird K, Choocherd S, Chimnoi W, Klinkaew N, Kengradomkij C, Phoosangwalthong P, Thammasonthijarern N, Pattanatanang K, Inpankaew T, Phasuk J, Nimsuphan B. Molecular Identification of Culicoides Species and Host Preference Blood Meal in the African Horse Sickness Outbreak-Affected Area in Hua Hin District, Prachuap Khiri Khan Province, Thailand. Insects 2023 Apr 8;14(4).
    doi: 10.3390/insects14040369pubmed: 37103184google scholar: lookup
  21. Adesola RO, Bakre AA, Gulumbe BH. Addressing the recent outbreak of African horse sickness in Lagos, Nigeria. Front Vet Sci 2023;10:1160856.
    doi: 10.3389/fvets.2023.1160856pubmed: 37065236google scholar: lookup
  22. Duan YL, Yang ZX, He YW, Li L. Two putative novel serotypes of Tibet orbivirus isolated from Culicoides spp. in Yunnan, China. J Vet Sci 2023 Mar;24(2):e18.
    doi: 10.4142/jvs.22194pubmed: 37012028google scholar: lookup
  23. Taesuji M, Rattanamas K, Kulthonggate U, Mamom T, Ruenphet S. Sensitivity and specificity for African horse sickness antibodies detection using monovalent and polyvalent vaccine antigen-based dot blotting. Vet World 2022 Dec;15(12):2760-2763.
    doi: 10.14202/vetworld.2022.2760-2763pubmed: 36718334google scholar: lookup
  24. Fujisawa Y, Kornmatitsuk K, Kornmatitsuk S, Kornmatitsuk B. Field evaluation of newly developed 3D-printed ultraviolet and green light-emitting diode traps for the collection of Culicoides species in Thailand. PLoS One 2023;18(1):e0280673.
    doi: 10.1371/journal.pone.0280673pubmed: 36662802google scholar: lookup
  25. Rattanamas K, Taesuji M, Kulthonggate U, Jantafong T, Mamom T, Ruenphet S. Sensitivity of RNA viral nucleic acid-based detection of avian influenza virus, Newcastle disease virus, and African horse sickness virus on flinders technology associates card using conventional reverse-transcription polymerase chain reaction. Vet World 2022 Nov;15(11):2754-2759.
    doi: 10.14202/vetworld.2022.2754-2759pubmed: 36590111google scholar: lookup
  26. Wang Y, Ong J, Ng OW, Songkasupa T, Koh EY, Wong JPS, Puangjinda K, Fernandez CJ, Huangfu T, Ng LC, Chang SF, Yap HH. Development of Differentiating Infected from Vaccinated Animals (DIVA) Real-Time PCR for African Horse Sickness Virus Serotype 1. Emerg Infect Dis 2022 Dec;28(12):2446-2454.
    doi: 10.3201/eid2812.220594pubmed: 36417933google scholar: lookup
  27. Durán-Ferrer M, Villalba R, Fernández-Pacheco P, Tena-Tomás C, Jiménez-Clavero MÁ, Bouzada JA, Ruano MJ, Fernández-Pinero J, Arias M, Castillo-Olivares J, Agüero M. Clinical, Virological and Immunological Responses after Experimental Infection with African Horse Sickness Virus Serotype 9 in Immunologically Naïve and Vaccinated Horses. Viruses 2022 Jul 15;14(7).
    doi: 10.3390/v14071545pubmed: 35891525google scholar: lookup
  28. Stokes JE, Carpenter S, Sanders C, Gubbins S. Emergence dynamics of adult Culicoides biting midges at two farms in south-east England. Parasit Vectors 2022 Jul 11;15(1):251.
    doi: 10.1186/s13071-022-05370-zpubmed: 35820957google scholar: lookup
  29. Tsai KJ, Tu YC, Wu CH, Huang CW, Ting LJ, Huang YL, Pan CH, Chang CY, Deng MC, Lee F. First detection and phylogenetic analysis of lumpy skin disease virus from Kinmen Island, Taiwan in 2020. J Vet Med Sci 2022 Aug 1;84(8):1093-1100.
    doi: 10.1292/jvms.21-0649pubmed: 35691931google scholar: lookup
  30. Gao S, Zeng Z, Wang H, Chen F, Huang L, Wang X. Predicting the possibility of African horse sickness (AHS) introduction into China using spatial risk analysis and habitat connectivity of Culicoides. Sci Rep 2022 Mar 10;12(1):3910.
    doi: 10.1038/s41598-022-07512-wpubmed: 35273211google scholar: lookup
  31. Goffredo M, Quaglia M, De Ascentis M, d'Alessio SG, Federici V, Conte A, Venter GJ. The Absence of Abdominal Pigmentation in Livestock Associated Culicoides following Artificial Blood Feeding and the Epidemiological Implication for Arbovirus Surveillance. Pathogens 2021 Dec 2;10(12).
    doi: 10.3390/pathogens10121571pubmed: 34959526google scholar: lookup
  32. Plangsangmas T, Rattanathanya H, Tipkantha W, Sanannu S, Maikaew U, Thaeonoen J, Sangkharak B, Chinson P, Hin-On W, Kanatiyanont N, Siriaroonrat B, Chansue N, Ratanakorn P. Immobilization of captive plains zebras (Equus quagga) with a combination of etorphine hydrochloride, acepromazine, and xylazine hydrochloride. J Vet Med Sci 2022 Jan 24;84(1):181-185.
    doi: 10.1292/jvms.21-0458pubmed: 34866094google scholar: lookup
  33. Gao H, Wang L, Ma J, Gao X, Xiao J, Wang H. Modeling the current distribution suitability and future dynamics of Culicoides imicola under climate change scenarios. PeerJ 2021;9:e12308.
    doi: 10.7717/peerj.12308pubmed: 34760364google scholar: lookup
  34. Knox A, Beddoe T. Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens. Animals (Basel) 2021 Jul 20;11(7).
    doi: 10.3390/ani11072150pubmed: 34359278google scholar: lookup
  35. Clemmons EA, Alfson KJ, Dutton JW 3rd. Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences. Animals (Basel) 2021 Jul 8;11(7).
    doi: 10.3390/ani11072039pubmed: 34359167google scholar: lookup
  36. Bunpapong N, Charoenkul K, Nasamran C, Chamsai E, Udom K, Boonyapisitsopa S, Tantilertcharoen R, Kesdangsakonwut S, Techakriengkrai N, Suradhat S, Thanawongnuwech R, Amonsin A. African Horse Sickness Virus Serotype 1 on Horse Farm, Thailand, 2020. Emerg Infect Dis 2021 Aug;27(8):2208-2211.
    doi: 10.3201/eid2708.210004pubmed: 34287126google scholar: lookup
  37. Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Depner K, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar Schmidt C, Herskin M, Michel V, Miranda Chueca MÁ, Pasquali P, Roberts HC, Sihvonen LH, Spoolder H, Ståhl K, Velarde A, Viltrop A, Winckler C, De Clercq K, Klement E, Stegeman JA, Gubbins S, Antoniou SE, Broglia A, Van der Stede Y, Zancanaro G, Aznar I. Scientific Opinion on the assessment of the control measures of the category A diseases of Animal Health Law: African Horse Sickness. EFSA J 2021 Feb;19(2):e06403.
    doi: 10.2903/j.efsa.2021.6403pubmed: 33552302google scholar: lookup
  38. Raksakoon C, Potiwat R. Current Arboviral Threats and Their Potential Vectors in Thailand. Pathogens 2021 Jan 18;10(1).
    doi: 10.3390/pathogens10010080pubmed: 33477699google scholar: lookup
  39. Tugwell LA, England ME, Gubbins S, Sanders CJ, Stokes JE, Stoner J, Graham SP, Blackwell A, Darpel KE, Carpenter S. Thermal limits for flight activity of field-collected Culicoides in the United Kingdom defined under laboratory conditions. Parasit Vectors 2021 Jan 18;14(1):55.
    doi: 10.1186/s13071-020-04552-xpubmed: 33461612google scholar: lookup
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