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Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2014; 26(6); 788-794; doi: 10.1177/1040638714553428

Outbreaks of Vesicular stomatitis Alagoas virus in horses and cattle in northeastern Brazil.

Abstract: The current article describes outbreaks of vesicular stomatitis (VS) in horses and cattle in Paraiba and Rio Grande do Norte states, northeastern Brazil, between June and August 2013. The reported cases affected 15-20 horses and 6 cattle distributed over 6 small farms in 4 municipalities, but additional data indicated the involvement of a large number of animals on several farms. The disease was characterized by blisters; eruptive lesions in coronary bands, lips, mouth, and muzzle; salivation; claudication and loss of condition. Swollen lower limbs and lips, and ulcerated and erosive areas in the lips and muzzle were observed in some horses. A necrotizing vesiculopustular dermatitis and stomatitis was observed histologically. Vesicular stomatitis virus was isolated from the vesicular fluid of a horse lesion and shown to be serologically related to the VS Indiana serogroup (VSIV) by virus neutralization. Convalescent sera of affected horses and cattle, and from healthy contacts, harbored high levels of neutralizing antibodies against the isolated virus (named VSIV-3 2013SaoBento/ParaibaE). Genomic sequences of VSIV subtype 3 (Vesicular stomatitis Alagoas virus) were amplified by reverse transcription polymerase chain reaction out of clinical specimens from a cow and a horse from different farms. Nucleotide sequencing and phylogenetic analysis of the phosphoprotein gene indicated that the 2 isolates were derived from the same virus and clustered them in VSIV-3, along with VS viruses identified in southeastern and northeastern Brazil in the last decades. Thus, the present report demonstrates the circulation of VSIV-3 in northeastern Brazil and urges for more effective diagnosis and surveillance.
© 2014 The Author(s).
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Publication Date: 2014-10-01 PubMed ID: 25274744DOI: 10.1177/1040638714553428Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article outlines an outbreak of Vesicular stomatitis Alagoas virus in horses and cattle in northeastern Brazil, noting the symptoms presented, and identifying the specific subtype of the virus involved.

Outbreak Details

  • The study pinpoints the spread of vesicular stomatitis (VS) virus in Brazil during June and August 2013. The major locales affected were Paraiba and Rio Grande do Norte states in northeastern Brazil.
  • The reported cases involved 15-20 horses and 6 cattle across 6 small farms in 4 municipalities. There is an implication that more unreported cases occurred given that additional data indicated the wide presence of disease across numerous farms.

Symptoms and Observations

  • The animals, both horses and cattle, showed signs of blisters; eruptive lesions in coronary bands, lips, mouth, and muzzle; salivation; claudication, and loss of condition.
  • Some horses had symptoms of swollen lower limbs and lips, and ulcerated and erosive areas in the lips and muzzle.
  • A histological examination revealed examples of necrotizing vesiculopustular dermatitis and stomatitis.
  • The vesicular stomatitis virus was isolated from the vesicular fluid of a horse lesion.

Identification of the Virus

  • Serological analysis through virus neutralization identified the virus as related to the VS Indiana serogroup (VSIV).
  • The isolated virus was named VSIV-3 2013SaoBento/ParaibaE based on the location and timing of the outbreak.
  • Protective responses in the form of neutralizing antibodies against the virus were found in convalescent sera of affected animals, both horses, and cattle, as well as healthy contacts. This shows the immune system’s active response against the viral infection.

Sequencing and Analysis

  • By using a method called reverse transcription polymerase chain reaction, genomic sequences of VSIV subtype 3 (Vesicular stomatitis Alagoas virus) were amplified from clinical specimens from a cow and a horse.
  • Through nucleotide sequencing and phylogenetic analysis of the phosphoprotein gene, it was determined that the two isolates had originated from the same virus and clustered in VSIV-3, confirming the presence of VS Alagoas virus.

Conclusion

  • This study concluded with an affirmation of the circulation of VSIV-3, Vesicular stomatitis Alagoas virus, in northeastern Brazil. The researchers stress on the need for enhanced diagnosis and surveillance to manage further spread of this disease.

Cite This Article

APA
Cargnelutti JF, Olinda RG, Maia LA, de Aguiar GM, Neto EG, Simões SV, de Lima TG, Dantas AF, Weiblen R, Flores EF, Riet-Correa F. (2014). Outbreaks of Vesicular stomatitis Alagoas virus in horses and cattle in northeastern Brazil. J Vet Diagn Invest, 26(6), 788-794. https://doi.org/10.1177/1040638714553428

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 26
Issue: 6
Pages: 788-794

Researcher Affiliations

Cargnelutti, Juliana F
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Olinda, Roberio G
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Maia, Lisanka A
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
de Aguiar, Gildeni M N
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Neto, Eldinê G M
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Simões, Sara V D
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
de Lima, Tatiane G
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Dantas, Antônio F M
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Weiblen, Rudi
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Flores, Eduardo F
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Riet-Correa, Franklin
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa) franklin.riet@pq.cnpq.br.

MeSH Terms

  • Animals
  • Brazil / epidemiology
  • Cattle
  • Cattle Diseases / diagnosis
  • Cattle Diseases / epidemiology
  • Cattle Diseases / virology
  • Disease Outbreaks / veterinary
  • Female
  • Horse Diseases / diagnosis
  • Horse Diseases / epidemiology
  • Horse Diseases / virology
  • Horses
  • Male
  • Molecular Sequence Data
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Sequence Analysis, DNA / veterinary
  • Vesicular Stomatitis / diagnosis
  • Vesicular Stomatitis / epidemiology
  • Vesicular Stomatitis / virology
  • Vesicular stomatitis Indiana virus / classification
  • Vesicular stomatitis Indiana virus / genetics
  • Vesicular stomatitis Indiana virus / isolation & purification
  • Vesiculovirus / classification
  • Vesiculovirus / genetics
  • Vesiculovirus / isolation & purification
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Citations

This article has been cited 8 times.
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    doi: 10.3389/fcimb.2025.1499658pubmed: 40078875google scholar: lookup
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