FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / J Vet Diagn Invest / Outbreaks of Vesicular stomatitis Alagoas virus in horses an...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2014; 26(6); 788-794; doi: 10.1177/1040638714553428

Outbreaks of Vesicular stomatitis Alagoas virus in horses and cattle in northeastern Brazil.

Abstract: The current article describes outbreaks of vesicular stomatitis (VS) in horses and cattle in Paraiba and Rio Grande do Norte states, northeastern Brazil, between June and August 2013. The reported cases affected 15-20 horses and 6 cattle distributed over 6 small farms in 4 municipalities, but additional data indicated the involvement of a large number of animals on several farms. The disease was characterized by blisters; eruptive lesions in coronary bands, lips, mouth, and muzzle; salivation; claudication and loss of condition. Swollen lower limbs and lips, and ulcerated and erosive areas in the lips and muzzle were observed in some horses. A necrotizing vesiculopustular dermatitis and stomatitis was observed histologically. Vesicular stomatitis virus was isolated from the vesicular fluid of a horse lesion and shown to be serologically related to the VS Indiana serogroup (VSIV) by virus neutralization. Convalescent sera of affected horses and cattle, and from healthy contacts, harbored high levels of neutralizing antibodies against the isolated virus (named VSIV-3 2013SaoBento/ParaibaE). Genomic sequences of VSIV subtype 3 (Vesicular stomatitis Alagoas virus) were amplified by reverse transcription polymerase chain reaction out of clinical specimens from a cow and a horse from different farms. Nucleotide sequencing and phylogenetic analysis of the phosphoprotein gene indicated that the 2 isolates were derived from the same virus and clustered them in VSIV-3, along with VS viruses identified in southeastern and northeastern Brazil in the last decades. Thus, the present report demonstrates the circulation of VSIV-3 in northeastern Brazil and urges for more effective diagnosis and surveillance.
© 2014 The Author(s).
Get Full Text
Publication Date: 2014-10-01 PubMed ID: 25274744DOI: 10.1177/1040638714553428Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Case Reports
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article outlines an outbreak of Vesicular stomatitis Alagoas virus in horses and cattle in northeastern Brazil, noting the symptoms presented, and identifying the specific subtype of the virus involved.

Outbreak Details

  • The study pinpoints the spread of vesicular stomatitis (VS) virus in Brazil during June and August 2013. The major locales affected were Paraiba and Rio Grande do Norte states in northeastern Brazil.
  • The reported cases involved 15-20 horses and 6 cattle across 6 small farms in 4 municipalities. There is an implication that more unreported cases occurred given that additional data indicated the wide presence of disease across numerous farms.

Symptoms and Observations

  • The animals, both horses and cattle, showed signs of blisters; eruptive lesions in coronary bands, lips, mouth, and muzzle; salivation; claudication, and loss of condition.
  • Some horses had symptoms of swollen lower limbs and lips, and ulcerated and erosive areas in the lips and muzzle.
  • A histological examination revealed examples of necrotizing vesiculopustular dermatitis and stomatitis.
  • The vesicular stomatitis virus was isolated from the vesicular fluid of a horse lesion.

Identification of the Virus

  • Serological analysis through virus neutralization identified the virus as related to the VS Indiana serogroup (VSIV).
  • The isolated virus was named VSIV-3 2013SaoBento/ParaibaE based on the location and timing of the outbreak.
  • Protective responses in the form of neutralizing antibodies against the virus were found in convalescent sera of affected animals, both horses, and cattle, as well as healthy contacts. This shows the immune system’s active response against the viral infection.

Sequencing and Analysis

  • By using a method called reverse transcription polymerase chain reaction, genomic sequences of VSIV subtype 3 (Vesicular stomatitis Alagoas virus) were amplified from clinical specimens from a cow and a horse.
  • Through nucleotide sequencing and phylogenetic analysis of the phosphoprotein gene, it was determined that the two isolates had originated from the same virus and clustered in VSIV-3, confirming the presence of VS Alagoas virus.

Conclusion

  • This study concluded with an affirmation of the circulation of VSIV-3, Vesicular stomatitis Alagoas virus, in northeastern Brazil. The researchers stress on the need for enhanced diagnosis and surveillance to manage further spread of this disease.

Cite This Article

APA
Cargnelutti JF, Olinda RG, Maia LA, de Aguiar GM, Neto EG, Simões SV, de Lima TG, Dantas AF, Weiblen R, Flores EF, Riet-Correa F. (2014). Outbreaks of Vesicular stomatitis Alagoas virus in horses and cattle in northeastern Brazil. J Vet Diagn Invest, 26(6), 788-794. https://doi.org/10.1177/1040638714553428

Publication

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
NlmUniqueID: 9011490
Country: United States
Language: English
Volume: 26
Issue: 6
Pages: 788-794

Researcher Affiliations

Cargnelutti, Juliana F
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Olinda, Roberio G
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Maia, Lisanka A
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
de Aguiar, Gildeni M N
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Neto, Eldinê G M
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Simões, Sara V D
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
de Lima, Tatiane G
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Dantas, Antônio F M
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Weiblen, Rudi
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Flores, Eduardo F
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa).
Riet-Correa, Franklin
  • Setor de Virologia, Department of Preventive Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (Cargnelutti, de Lima, Weiblen, Flores)Veterinary Hospital, Centro de Saúde e Tecnologia Rural, Federal University of Campina Grande, Campus de Patos, Patos, Paraíba, Brazil (Olinda, Maia, de Aguiar, Neto, Simões, Dantas, Riet-Correa) franklin.riet@pq.cnpq.br.

MeSH Terms

  • Animals
  • Brazil / epidemiology
  • Cattle
  • Cattle Diseases / diagnosis
  • Cattle Diseases / epidemiology
  • Cattle Diseases / virology
  • Disease Outbreaks / veterinary
  • Female
  • Horse Diseases / diagnosis
  • Horse Diseases / epidemiology
  • Horse Diseases / virology
  • Horses
  • Male
  • Molecular Sequence Data
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Sequence Analysis, DNA / veterinary
  • Vesicular Stomatitis / diagnosis
  • Vesicular Stomatitis / epidemiology
  • Vesicular Stomatitis / virology
  • Vesicular stomatitis Indiana virus / classification
  • Vesicular stomatitis Indiana virus / genetics
  • Vesicular stomatitis Indiana virus / isolation & purification
  • Vesiculovirus / classification
  • Vesiculovirus / genetics
  • Vesiculovirus / isolation & purification
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Citations

This article has been cited 8 times.
  1. Cavalcante MSB, Santos DS, Araújo LM, Freitas PL, Silva CAM, Carvalho KGB, Araújo MTF, da Silva EVP, Rodrigues de Farias APD, Guerreiro Diniz D, Picanço Diniz CW, Diniz JAP. Inflammatory and neuropathological responses to Vesiculovirus carajas encephalitis in adult mice: variability, tolerance and resistance. Front Cell Infect Microbiol 2025;15:1499658.
    doi: 10.3389/fcimb.2025.1499658pubmed: 40078875google scholar: lookup
  2. Vasco-Julio D, Aguilar D, Maldonado A, de la Torre E, Cisneros-Montufar MS, Bastidas-Caldes C, Navarro JC, de Waard JH. Molecular Tracking of the Origin of Vesicular Stomatitis Outbreaks in 2004 and 2018, Ecuador. Vet Sci 2023 Feb 24;10(3).
    doi: 10.3390/vetsci10030181pubmed: 36977220google scholar: lookup
  3. Sales ML, Fonseca Júnior AA, Pinheiro de Oliveira TF, de Araújo Lopes A, Rivetti Júnior AV, Camargos MF, Dos Reis JKP. Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR. Braz J Microbiol 2023 Mar;54(1):491-497.
    doi: 10.1007/s42770-023-00902-wpubmed: 36645640google scholar: lookup
  4. Fonseca Júnior AA, Laguardia-Nascimento M, Barbosa AAS, da Silva Gonçalves VL, de Oliveira AM, Rivetti Júnior AV, Camargos MF. Phylodynamics of Alagoas vesiculovirus in Brazil. Braz J Microbiol 2022 Sep;53(3):1691-1699.
    doi: 10.1007/s42770-022-00756-8pubmed: 35553417google scholar: lookup
  5. Kakuk B, Kiss AA, Torma G, Csabai Z, Prazsák I, Mizik M, Megyeri K, Tombácz D, Boldogkői Z. Nanopore Assay Reveals Cell-Type-Dependent Gene Expression of Vesicular Stomatitis Indiana Virus and Differential Host Cell Response. Pathogens 2021 Sep 15;10(9).
    doi: 10.3390/pathogens10091196pubmed: 34578228google scholar: lookup
  6. de Oliveira AM, Laguardia-Nascimento M, Sales ML, Júnior ARV, Camargos MF, de Melo CB, Fonseca Júnior AA. Outbreaks of Vesicular Stomatitis in Brazil caused by a distinct lineage of Alagoas vesiculovirus. Braz J Microbiol 2021 Sep;52(3):1637-1642.
    doi: 10.1007/s42770-021-00537-9pubmed: 34145554google scholar: lookup
  7. Kumar B, Manuja A, Gulati BR, Virmani N, Tripathi BN. Zoonotic Viral Diseases of Equines and Their Impact on Human and Animal Health. Open Virol J 2018;12:80-98.
    doi: 10.2174/1874357901812010080pubmed: 30288197google scholar: lookup
  8. Tolardo AL, Souza WM, Romeiro MF, Vieira LC, Luna LK, Henriques DA, Araujo Jd, Siqueira CE, Colombo TE, Aquino VH, Fonseca BA, Bronzoni RV, Nogueira ML, Durigon EL, Figueiredo LT. A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus. Mem Inst Oswaldo Cruz 2016 Jun 3;111(6):385-90.
    doi: 10.1590/0074-02760150456pubmed: 27276185google scholar: lookup
Subscribe to our newsletter
Join 99,359 horse owners like you who receive our email updates on horse health and nutrition.