Ovarian steroids affect prostaglandin production in equine endometrial cells in vitro.
Abstract: This study aimed to evaluate the influence of ovarian steroids on equine endometrial epithelial and stromal cells, specifically i) prostaglandin (PG) production in a time-dependent manner, ii) specific PG synthases mRNA transcription and protein expression, and iii) cell proliferation. After passage I, cells were exposed to vehicle, oxytocin (OT, positive control, 10(-7) M), progesterone (P4, 10(-7) M), 17β estradiol (E2, 10(-9) M), or P4+E2 for 12, 24, 48, or 72 h. Following treatment, PG concentration was determined using the direct enzyme immunoassay (EIA) method. Alterations in PG synthases mRNA transcriptions, PG synthases protein expression, and cell proliferation in response to the treatments were determined after 24 h using real-time PCR, western blot, or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide respectively. After 24 h, E2 and P4+E2 increased PGE2 and PGF2α secretion as well as specific prostaglandin-endoperoxide synthase-2 (PTGS2), PGE2 synthases (PGES), and PGF2α synthases (PGFS) expression in the epithelial cells (P<0.05). Additionally, E2 and P4+E2 increased PTGS2 expression in stromal cells after 24 h (P<0.05). In stromal cells, P4+E2 increased PGE2 production as well as PGES expression after 24 h (P<0.05). Both E2 and P4+E2 increased PGF2α production by stromal cells after 24 h (P<0.05). Ovarian steroids affected proliferation of stromal and epithelial cells during the 24-h incubation period (P<0.05). We provide evidence that ovarian steroids affect PG production in equine endometrial cells, upregulating PTGS2, PGES, and PGFS expression. Ovarian steroid-stimulated PG production could be an important mechanism occurring in the equine endometrium that is involved in the regulation of the estrous cycle and early pregnancy.
Publication Date: 2014-01-30 PubMed ID: 24481966DOI: 10.1530/JOE-13-0185Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research focuses on exploring how ovarian steroids impact the production of prostaglandin (a hormone-like substance) in equine endometrial cells in a lab setting.
Methodology
- The experiment looked at the roles of ovarian steroids on equine endometrial epithelial and stromal cells, with a particular attention on prostaglandin production over time, the transcription and protein expression of specific prostaglandin synthases (structures that aid in the production of prostaglandins), and the proliferation of cells.
- Cells were exposed to certain substances, including oxytocin as a positive control, progesterone, 17β estradiol, or a combination of progesterone and estradiol for varying periods up to 72 hours.
- After the treatments, the concentration of prostaglandin was recorded using a method known as a direct enzyme immunoassay. Changes in prostaglandin synthase mRNA transcriptions, protein expressions, and cell proliferation following treatments were studied after 24 hours using real-time PCR, western blot, and a form of cell viability assay respectively.
Findings
- The researchers found that after 24 hours, 17β estradiol and the combined treatment of progesterone and 17β estradiol increased the secretion of certain prostaglandins as well as the expression of specific prostaglandin syntheses in epithelial cells.
- These treatment also saw an increase in the expression of a specific prostaglandin synthase in stromal cells after 24 hours.
- For stromal cells, the combined treatment increased the production of certain prostaglandins along with the expression of a particular prostaglandin synthase after 24 hours.
- Both the 17β estradiol treatment and the combined treatment boosted production of certain prostaglandin by stromal cells after 24 hours.
- All ovarian steroids affected the proliferation of both types of cells over a 24-hour period.
Conclusion
- The researchers concluded that ovarian steroids influence prostaglandin production in equine endometrial cells, with increased expression of certain prostaglandin syntheses.
- The researchers suggest this process could be pivotal in the regulation of the estrous cycle and early pregnancy in horses.
Cite This Article
APA
Szóstek AZ, Galvão AM, Ferreira-Dias GM, Skarzynski DJ.
(2014).
Ovarian steroids affect prostaglandin production in equine endometrial cells in vitro.
J Endocrinol, 220(3), 263-276.
https://doi.org/10.1530/JOE-13-0185 Publication
Researcher Affiliations
- Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Olsztyn, Poland The Interdisciplinary Centre of Research in Animal Health, Faculty of Veterinary Medicine, Technical University of Lisbon, Lisbon, Portugal.
MeSH Terms
- Animals
- Cell Proliferation
- Cells, Cultured
- Endometrium / cytology
- Endometrium / enzymology
- Endometrium / metabolism
- Epithelial Cells / cytology
- Epithelial Cells / metabolism
- Estradiol / metabolism
- Female
- Horses
- Hydroxyprostaglandin Dehydrogenases / genetics
- Hydroxyprostaglandin Dehydrogenases / metabolism
- Ovary / enzymology
- Ovary / metabolism
- Pregnancy
- Progesterone / metabolism
- Prostaglandin-Endoperoxide Synthases / genetics
- Prostaglandin-Endoperoxide Synthases / metabolism
- Prostaglandins / metabolism
- Stromal Cells / cytology
- Stromal Cells / metabolism
Citations
This article has been cited 3 times.- Piotrowska-Tomala KK, Jonczyk AW, Szóstek-Mioduchowska AZ, Żebrowska E, Ferreira-Dias G, Skarzynski DJ. The Effects of Prostaglandin E(2) Treatment on the Secretory Function of Mare Corpus Luteum Depends on the Site of Application: An in vivo Study.. Front Vet Sci 2021;8:753796.
- Rebordão MR, Amaral A, Fernandes C, Silva E, Lukasik K, Szóstek-Mioduchowska A, Pinto-Bravo P, Galvão A, Skarzynski DJ, Ferreira-Dias G. Enzymes Present in Neutrophil Extracellular Traps May Stimulate the Fibrogenic PGF(2α) Pathway in the Mare Endometrium.. Animals (Basel) 2021 Sep 6;11(9).
- Szóstek AZ, Galvão AM, Hojo T, Okuda K, Skarzynski DJ. Interleukins affect equine endometrial cell function: modulatory action of ovarian steroids.. Mediators Inflamm 2014;2014:208103.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
