Ovine anti-rabies antibody production and evaluation.
Abstract: In view of the disadvantages of human and equine rabies immunoglobulin still there is urgent needs for safe and cost-control anti-rabies immunoglobulins especially for person who have been severely exposed (categories III) to the virus. Our attempt to produce a less immunogenic and cheaper anti-rabies immunoglobulin affordable for those people living in developing countries, has been harnessed the ovine as a bioreactor instead the horse. The animals have been intramuscular immunized, and the plasma processed with 5% caprylic acid to yield IgG with purity of 95%. Moreover, antibody apparently indicated that the titer and neutralizing indexes were harmonized, especially at higher antibody dilution. The results showed that three immunized sheep were produced about 7000 IU of purified anti-rabies antibody. Sheep's IgG has low immunogenic effect than human and horse antibodies when injected into the mouse. Pure concentrated ovine antibody may serve as a possible alternative to currently available anti-rabies human or equine immunoglobulin.
Publication Date: 2008-02-19 PubMed ID: 18242705DOI: 10.1016/j.cimid.2007.11.004Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The article presents research about using sheep as a bioreactor to produce a less allergenic and more affordable anti-rabies immunoglobulin. This could potentially offer a safer and more cost-effective treatment for individuals who have been severely exposed to the rabies virus, particularly in developing countries.
Production of Ovine Anti-Rabies Antibody
- The study attempts to create anti-rabies immunoglobulins that are less allergenic and more affordable, specifically for individuals in developing countries who have been severely exposed to the rabies virus.
- Instead of using horses, the researchers used sheep as a bioreactor to produce the immunoglobulins.
- The sheep were immunized intramuscularly, and their plasma was then processed with 5% caprylic acid, resulting in Immunoglobulin G (IgG) with 95% purity.
Antibody Titer and Neutralizing Indexes
- The study found that the titer (concentration) and neutralizing indexes (the ability of the antibodies to neutralize the virus) were in harmony, especially at higher antibody dilutions.
- Three immunized sheep produced approximately 7000 international units (IU) of purified anti-rabies antibodies.
- These findings suggest a successful production and a high efficacy of the ovine-derived anti-rabies antibodies.
Comparative Evaluation and Potential Application
- When injected into mice, the sheep’s Immunoglobulin G (IgG) showed lower allergenic effects compared to antibodies from humans and horses.
- This indicates that ovine antibodies are potentially safer and less allergenic for use in rabies post-exposure prophylaxis.
- Hence, pure concentrated ovine antibodies may serve as feasible alternatives to the currently available anti-rabies immunoglobulins derived from humans or horses.
Cite This Article
APA
Redwan el-RM, Fahmy A, El Hanafy A, Abd El-Baky N, Sallam SM.
(2008).
Ovine anti-rabies antibody production and evaluation.
Comp Immunol Microbiol Infect Dis, 32(1), 9-19.
https://doi.org/10.1016/j.cimid.2007.11.004 Publication
Researcher Affiliations
- Protein Research Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt. redwan1961@yahoo.com
MeSH Terms
- Animals
- Antibodies, Viral / biosynthesis
- Antibodies, Viral / immunology
- Antibodies, Viral / isolation & purification
- Camelus / immunology
- Chlorocebus aethiops
- Cross Reactions / immunology
- Female
- Horses / immunology
- Humans
- Immunization
- Immunization, Passive
- Immunoglobulin G / immunology
- Mice
- Neutralization Tests
- Rabbits
- Rabies / prevention & control
- Rabies Vaccines / immunology
- Rabies virus / immunology
- Sheep, Domestic / immunology
- Vero Cells
Citations
This article has been cited 8 times.- Findlay-Wilson S, Easterbrook L, Smith S, Pope N, Humphries G, Schuhmann H, Ngabo D, Rayner E, Otter AD, Coleman T, Hicks B, Graham VA, Halkerston R, Apostolakis K, Taylor S, Fotheringham S, Horton A, Tree JA, Wand M, Hewson R, Dowall SD. Development of a cost-effective ovine antibody-based therapy against SARS-CoV-2 infection and contribution of antibodies specific to the spike subunit proteins.. Antiviral Res 2022 Jul;203:105332.
- Redwan EM, Aljadawi AA, Uversky VN. Simple and efficient protocol for immunoglobulin Y purification from chicken egg yolk.. Poult Sci 2021 Mar;100(3):100956.
- El-Fakharany EM, El-Baky NA, Linjawi MH, Aljaddawi AA, Saleem TH, Nassar AY, Osman A, Redwan EM. Influence of camel milk on the hepatitis C virus burden of infected patients.. Exp Ther Med 2017 Apr;13(4):1313-1320.
- Zarei AE, Almehdar HA, Redwan EM. Hib Vaccines: Past, Present, and Future Perspectives.. J Immunol Res 2016;2016:7203587.
- Dowall SD, Callan J, Zeltina A, Al-Abdulla I, Strecker T, Fehling SK, Krähling V, Bosworth A, Rayner E, Taylor I, Charlton S, Landon J, Cameron I, Hewson R, Nasidi A, Bowden TA, Carroll MW. Development of a Cost-effective Ovine Polyclonal Antibody-Based Product, EBOTAb, to Treat Ebola Virus Infection.. J Infect Dis 2016 Apr 1;213(7):1124-33.
- Stevens NE, Fraser CK, Alsharifi M, Brown MP, Diener KR, Hayball JD. An empirical approach towards the efficient and optimal production of influenza-neutralizing ovine polyclonal antibodies demonstrates that the novel adjuvant CoVaccine HT™ is functionally superior to Freund's adjuvant.. PLoS One 2013;8(7):e68895.
- El-Fakharany EM, Abedelbaky N, Haroun BM, Sánchez L, Redwan NA, Redwan EM. Anti-infectivity of camel polyclonal antibodies against hepatitis C virus in Huh7.5 hepatoma.. Virol J 2012 Sep 16;9:201.
- Liu X, Lin H, Tang Q, Li C, Yang S, Wang Z, Wang C, He Q, Cao B, Feng Z, Guan X, Zhu J. Characterization of a human antibody fragment Fab and its calcium phosphate nanoparticles that inhibit rabies virus infection with vaccine.. PLoS One 2011 May 9;6(5):e19848.
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