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Home / Equine Research Bank / Antioxidants (Basel) / Oxidant and Antioxidant Parameters’ Assessment Togethe...
Antioxidants (Basel, Switzerland)2022; 11(6); 1176; doi: 10.3390/antiox11061176

Oxidant and Antioxidant Parameters’ Assessment Together with Homocysteine and Muscle Enzymes in Racehorses: Evaluation of Positive Effects of Exercise.

Abstract: This study aimed to evaluate the changes in serum oxidant and antioxidant parameters together with the serum values of homocysteine (Hcy) and muscle enzymes including creatine kinase (CK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in regularly trained athletic horses during official races of 1200, 1600 and 2000 m. Thirty Thoroughbred horses were divided into three groups of 10 subjects each according to the race distance: Group 1, 1200 m race; Group 2, 1600 m race; Group 3, 2000 m race. Blood samples were collected from horses 1 week prior to the race (1WB), on the day of the race at rest (TREST), immediately after the race (TPOST), and after 30 (TPOST30) and 120 (TPOST120) minutes. Serum total proteins, reactive oxygen metabolites (dROMs), thiol antioxidant barrier (SHp), antioxidant barrier (Oxy-ads), Hcy, CK, AST and LDH values were assessed. A two-way repeated measures ANOVA did not show differences referable to the race distance (Group effect) on all investigated parameters (p > 0.05). An exercise effect on oxidative stress markers, Hcy and muscle enzymes herein investigated was found in all groups (p < 0.001). A Pearson’s test showed dROMs positively correlated with SHp, Oxy-ads and Hcy after exercise (p < 0.05). This study suggests that, though well-trained racing horses are subjected to oxidative stress during a race, a proper antioxidant capacity may improve their ability to cope with exercise-induced oxidative stress.
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Publication Date: 2022-06-15 PubMed ID: 35740073PubMed Central: PMC9220350DOI: 10.3390/antiox11061176Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the impacts of exercise on oxidant and antioxidant parameters, homocysteine, and muscle enzymes in trained racehorses. The study finds that exercising can induce oxidative stress in horses, but those with adequate antioxidant capacity can better manage it.

Study Objective and Methodology

  • The primary focus of this study was to observe the changes in serum oxidant and antioxidant parameters in trained racehorses during official races. The researchers evaluated the serum levels of homocysteine and muscle enzymes, namely creatine kinase, aspartate aminotransferase, and lactate dehydrogenase.
  • A selection of 30 Thoroughbred horses were divided into three groups of 10 each and made to run in races of different lengths: 1200 m, 1600 m, and 2000 m.
  • Blood samples were collected from the horses a week before the race, on the day of the race at rest, and at several intervals after the race—immediately, 30 minutes after, and two hours after. These samples were then examined for various health indicators.

Key Findings

  • The researchers noted no variation with regard to race distance on all studied parameters, as the two-way repeated measures ANOVA results showed. This suggests that the length of the race did not have a significant effect on the outcomes.
  • They discovered that exercise does have an observed effect on the oxidative stress markers, homocysteine, and muscle enzymes in all the studied groups.
  • A Pearson’s test showed a positive correlation between reactive oxygen metabolites (measured as dROMs) and the thiol antioxidant barrier, the antioxidant barrier, and homocysteine, after exercise.

Interpretation and Inference

  • The findings from this study indicate that well-trained racing horses experience oxidative stress during a race. Oxidative stress is an imbalance between free radicals and antioxidants in an organism’s body, which can lead to cell and tissue damage.
  • However, if the horse has a sufficient antioxidant capacity, it may be better able to cope with this exercise-induced oxidative stress. Antioxidants help by neutralizing the harmful effects of free radicals in the body.

Cite This Article

APA
(2022). Oxidant and Antioxidant Parameters’ Assessment Together with Homocysteine and Muscle Enzymes in Racehorses: Evaluation of Positive Effects of Exercise. Antioxidants (Basel), 11(6), 1176. https://doi.org/10.3390/antiox11061176

Publication

Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
NlmUniqueID: 101668981
Country: Switzerland
Language: English
Volume: 11
Issue: 6
PII: 1176

Researcher Affiliations

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 3 times.
  1. Gołyński M, Metyk M, Ciszewska J, Szczepanik MP, Fitch G, Bęczkowski PM. Homocysteine-Potential Novel Diagnostic Indicator of Health and Disease in Horses.. Animals (Basel) 2023 Apr 11;13(8).
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  3. Lo Feudo CM, Stucchi L, Conturba B, Stancari G, Ferrucci F. Impact of Lower Airway Inflammation on Fitness Parameters in Standardbred Racehorses.. Animals (Basel) 2022 Nov 21;12(22).
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