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Home / Equine Research Bank / Anim Genet / Parentage testing and linkage analysis in the horse using a ...
Animal genetics1994; 25(1); 19-23;

Parentage testing and linkage analysis in the horse using a set of highly polymorphic microsatellites.

Abstract: Ten (TG)n positive clones, isolated from an equine genomic library and sequenced, contained 12-19 uninterrupted TG repeats. Primers for polymerase chain reaction (PCR) were synthesized and nine of these (TG)n loci (HTG7-15) were successfully amplified and utilized in this study together with five previously reported equine microsatellite loci (HTG2-6). The PCR products were analysed by polyacrylamide gel electrophoresis followed by automated laser fluorescence detection or autoradiography. All microsatellites showed polymorphism and stable Mendelian inheritance. Differences in microsatellite variability between horse breeds were detected. A linkage analysis comprising HTG2-15, one coat colour gene and 16 genetic blood markers enabled addition of HTG2 to linkage group U2 and a new linkage group (U6) was established comprising the loci HTG7 and HTG12. Close linkage was excluded within a set of eight microsatellites. The estimated probability of exclusion in four breeds for a parentage test based on these eight loci varied between 0.96 and 0.99.
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Publication Date: 1994-02-01 PubMed ID: 8161016
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper involves the use of highly polymorphic microsatellites for parentage testing and linkage analysis in horses. Results show microsatellite variability between different horse breeds and a high probability of exclusion for a parentage test based on the selected loci.

Methodology

  • The study focused on the utilization of ten (TG)n positive clones derived from an equine genomic library. These were sequenced and showed 12-19 uninterrupted TG repeats.
  • For Polymerase Chain Reaction (PCR), primers were synthesized and applied to nine of the (TG)n loci, labelled as HTG7-15.
  • The research also included five previously outlined equine microsatellite loci, HTG2-6.
  • The PCR products were analyzed using polyacrylamide gel electrophoresis and detection was carried out either by automated laser fluorescence detection or autoradiography.

Findings

  • All analyzed microsatellites displayed polymorphism and stable Mendelian inheritance. Polymorphism here refers to the occurrence of different forms among the members of a population.
  • The study highlighted variabilities in microsatellite patterns amongst different horse breeds.
  • Linkage analysis involved HTG2-15, one coat color gene, and 16 genetic blood markers leading to the addition of HTG2 to the linkage group U2.
  • A new linkage group, known as U6, was established, which included the loci HTG7 and HTG12.
  • The study also ruled out close linkage within a set of eight microsatellites.
  • For a parentage test based on eight loci, the estimated probability of exclusion ranged from 0.96 to 0.99 across four different breeds. This high probability implies the effectiveness of these selected microsatellites in determining accurate parentage.

Implications

  • The findings from this study have significant implications for equine genetic research. The high power of exclusion in the parentage test indicates that the microsatellite markers can be reliably used to determine parentage in horses.
  • The observed variability in microsatellites across breeds could contribute to a better understanding of breed-specific traits and genetic diversity among horses.
  • The results could also help to improve existing equine linkage maps, contributing to a better understanding of horse genetics and potential genetic improvement programs within the industry.

Cite This Article

APA
Marklund S, Ellegren H, Eriksson S, Sandberg K, Andersson L. (1994). Parentage testing and linkage analysis in the horse using a set of highly polymorphic microsatellites. Anim Genet, 25(1), 19-23.

Publication

Animal genetics
ISSN: 0268-9146
NlmUniqueID: 8605704
Country: England
Language: English
Volume: 25
Issue: 1
Pages: 19-23

Researcher Affiliations

Marklund, S
  • Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala.
Ellegren, H
    Eriksson, S
      Sandberg, K
        Andersson, L

          MeSH Terms

          • Animals
          • Base Sequence
          • DNA, Satellite / genetics
          • Female
          • Genetic Linkage
          • Genetic Variation
          • Horses / genetics
          • Male
          • Molecular Sequence Data
          • Polymorphism, Genetic

          Citations

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