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Home / Equine Research Bank / J Vet Sci / Peripheral serotoninergic response to physical exercise in a...
Journal of veterinary science2010; 11(4); 285-289; doi: 10.4142/jvs.2010.11.4.285

Peripheral serotoninergic response to physical exercise in athletic horses.

Abstract: The purpose of this study was to evaluate the influence of exercise on plasma tryptophan (TRP) and free serotonin (f5-HT), whole blood-5-HT (WB-5-HT) and f5-HT/WB-5-HT ratio in Italian Saddle horses. Six clinically healthy Italian Saddle horses were subjected to a 450 meters obstacles course. Blood samples were collected from each horse by jugular venipuncture using vacutainer tubes with K(3)-EDTA at rest, immediately after exercise, and after 30 min. TRP, f5-HT and WB-5-HT were analyzed by HPLC. Immediately after exercise, statistically significant increases of f5-HT (p <0.001) and WB-5-HT (p <0.001) were observed. After 30 min, f5-HT and WB-5-HT decreased compared to immediately after exercise, but were still significantly higher than rest values (p <0.01 and p <0.05, respectively). A significant linear regression between f5-HT and WB-5-HT was observed during experimental conditions. f5-HT and WB-5-HT modifications after exercise suggest an important role of peripheral serotoninergic markers in response to physical activity. The possible source of extra serotonin detected after show jumping should be clarified by further investigation.
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Publication Date: 2010-11-30 PubMed ID: 21113096PubMed Central: PMC2998738DOI: 10.4142/jvs.2010.11.4.285Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focused on understanding how physical exercise impacts blood levels of tryptophan and serotonin in athletic horses, specifically, Italian Saddle horses. Results showed a significant increase in serotonin levels in the blood immediately after exercise, and though these levels decreased 30 minutes post-exercise, they were still higher than the resting levels.

Research Objective and Methodology

  • The goal of this study was to determine how physical activity affects the blood level concentrations of tryptophan (TRP), free serotonin (f5-HT), whole blood serotonin (WB-5-HT) and the ratio between free serotonin and whole blood serotonin (f5-HT/WB-5-HT) in Italian Saddle horses.
  • For the study, six healthy Italian Saddle horses were included. These horses were subjected to a 450-meter obstacle course, which represented the exercise component of the study.
  • Blood samples were collected from each horse before exercise (at rest), immediately after exercise, and 30 minutes post-exercise. These samples were collected using vacutainer tubes, a common tool for blood collection, with K(3)-EDTA, an anticoagulant that prevents blood clotting.
  • The collected blood samples were analyzed to measure the amounts of TRP, f5-HT, and WB-5-HT using high-performance liquid chromatography (HPLC), a technique used for separating components in a mixture, in this case, for isolating the substances of interest in blood samples.

Findings of the Study

  • The results of the study revealed significant increases in f5-HT and WB-5-HT levels in the blood immediately after the horses exercised.
  • However, 30 minutes after the exercise, the levels of f5-HT and WB-5-HT decreased, but were significantly higher than the resting values.
  • The study also found a significant linear regression relationship between f5-HT and WB-5-HT under the experimental conditions, indicating that as one increased, so did the other.
  • These findings suggest that peripheral serotoninergic markers (f5-HT and WB-5-HT) may play a crucial role in the body’s response to physical activity.
  • Yet, the researchers concluded more investigation is needed to clarify the origin of the extra serotonin detected in the horses’ blood after show jumping.

Cite This Article

APA
Alberghina D, Giannetto C, Piccione G. (2010). Peripheral serotoninergic response to physical exercise in athletic horses. J Vet Sci, 11(4), 285-289. https://doi.org/10.4142/jvs.2010.11.4.285

Publication

Journal of veterinary science
ISSN: 1976-555X
NlmUniqueID: 100964185
Country: Korea (South)
Language: English
Volume: 11
Issue: 4
Pages: 285-289

Researcher Affiliations

Alberghina, Daniela
  • Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, Messina, Italy. dalberghina@unime.it
Giannetto, Claudia
    Piccione, Giuseppe

      MeSH Terms

      • Animals
      • Biomarkers / blood
      • Female
      • Horses / blood
      • Horses / metabolism
      • Linear Models
      • Male
      • Physical Conditioning, Animal / physiology
      • Serotonin / blood
      • Tryptophan / blood

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