Persistent desensitisation of the beta 2 adrenoceptors expressed by cultured equine sweat gland epithelial cells.
Abstract: Adrenaline, forskolin and ATP all evoked accumulation of cyclic AMP in equine sweat gland epithelial cells, although the response to adrenaline was more transient than that to forskolin and ATP. Cells preincubated in adrenaline (10 micromol l-1, 32 min) showed essentially complete, homologous desensitisation, and this phenomenon reversed slowly (half-time 6.3+/-0.9 h). After 10 min of recovery from preincubation in adrenaline, isobutylmethylxanthine (IBMX, 5 mmol l-1) had no effect upon the desensitisation and the cells showed no loss of sensitivity to ATP and forskolin. After 10 h, however, the persistent desensitisation was partially reversed by IBMX and the cells showed reduced responses to ATP and forskolin. Increased phosphodiesterase activity may thus contribute to the persistent desensitisation. Experiments using forskolin-preincubated (100 micromol l-1, 32 min) cells suggested that increased cytosolic cyclic AMP levels did not underlie the initial loss of sensitivity to adrenaline but that this second messenger may initiate the series of events leading to the generalised loss of sensitivity seen after 10 h.
Publication Date: 1998-03-28 PubMed ID: 9405314DOI: 10.1242/jeb.201.2.259Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article focuses on how adrenaline, forskolin, and ATP interact on equine sweat gland epithelial cells, with adrenaline causing a transient desensitization that partially reversed with time and introduction of additional chemical compounds.
Overview of Findings
- This research investigated the reactions of equine sweat gland epithelial cells to the hormones adrenaline, forskolin, and ATP. Adrenaline showed a more transient response than the other hormones.
- Upon preincubation with adrenaline, the cells demonstrated a homologous desensitisation, essentially reducing their response. The desensitisation decreased slowly over time, with a half-life of approximately 6.3 hours.
Further Response to Chemical Agents
- The researchers introduced the substance isobutylmethylxanthine (IBMX) after 10 minutes of recovery from adrenaline preincubation. This had no effect on the desensitisation of the cells, and the cells showed no reduced sensitivity to ATP and forskolin.
- However, after 10 hours, the persistent desensitisation was partially reversed by the introduction of IBMX, and the cells showed reduced responses to ATP and forskolin.
Cyclic AMP and Phosphodiesterase Activity
- The researchers believe increased phosphodiesterase activity could be a contributing factor to the persistent desensitisation of these epithelial cells.
- Tests using cells that have been preincubated with forskolin suggest that the loss of sensitivity to adrenaline is not due to increased levels of the intracellular signaling molecule cyclic AMP.
- However, the researchers suggest that cyclic AMP could trigger a series of events leading to a general loss of sensitivity in the cell after 10 hours.
Implications of the Study
- This research provides deeper insight into the biochemical mechanisms of equine sweat gland epithelial cells. Specifically, it offers a better understanding of transient responses caused by adrenaline.
- The findings open doors to further exploration of the collective dynamics between hormones and sweat gland epithelial cells, potentially paving the way for advanced equine therapeutic approaches.
Cite This Article
APA
Rakhit S, Murdoch R, Wilson SM.
(1998).
Persistent desensitisation of the beta 2 adrenoceptors expressed by cultured equine sweat gland epithelial cells.
J Exp Biol, 201(Pt 2), 259-266.
https://doi.org/10.1242/jeb.201.2.259 Publication
Researcher Affiliations
- Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK.
MeSH Terms
- 1-Methyl-3-isobutylxanthine / pharmacology
- Animals
- Cell Line, Transformed
- Colforsin / pharmacology
- Cyclic AMP / metabolism
- Epinephrine / pharmacology
- Epithelial Cells / drug effects
- Epithelial Cells / metabolism
- Horses
- Protein Binding / drug effects
- Receptors, Adrenergic, beta-2 / biosynthesis
- Receptors, Adrenergic, beta-2 / drug effects
- Receptors, Adrenergic, beta-2 / metabolism
- Sweat Glands / cytology
- Sweat Glands / drug effects
- Sweat Glands / metabolism
- Time Factors
Citations
This article has been cited 1 times.- Ramminger SJ, Inglis SK, Olver RE, Wilson SM. Hormonal modulation of Na(+) transport in rat fetal distal lung epithelial cells.. J Physiol 2002 Oct 15;544(2):567-77.
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