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Home / Equine Research Bank / Korean J Parasitol / Phylogenetic Characteristics of Echinococcus granulosus Sens...
The Korean journal of parasitology2020; 58(2); 205-210; doi: 10.3347/kjp.2020.58.2.205

Phylogenetic Characteristics of Echinococcus granulosus Sensu Lato in Uzbekistan.

Abstract: Echinococcosis occurs mainly in areas with heavy livestock farming, such as Central Asia, America, and Australia. Echinococcus granulosus sensu lato (s.l.) infection causes echinococcosis in intermediate hosts, such as sheep, cattle, goats, camels, and horses. Numerous cases of echinococcosis occur in Uzbekistan as stock farming is a primary industry. Epidemiological and genetic studies of E. granulosus s.l. are very important for mitigating its impact on public health and the economy; however, there are no such studies on E. granulosus s.l. in Uzbekistan. In the present study, to determine which genotypes exist and are transmitted, we isolated Echinococcus sp. from definitive hosts (one isolate each from jackal and dog) and intermediate hosts (52 isolates from humans and 6 isolates from sheep) in Uzbekistan and analyzed the isolates by sequencing 2 mitochondrial DNA components (cox1 and nad1). The results showed that all of isolates except one belonged to the E. granulosus sensu stricto (s.s.) G1 and G3 genotypes. Phylogenetic analysis based on cox1 sequences showed that 42 isolates from humans, 6 isolates from sheep, and one isolate from jackal were the G1 genotype, whereas the remaining 8 isolates from human and the one isolate from dog were the G3 genotype. These results suggest that the G1 and G3 genotypes of E. granulosus s.s. are predominant in Uzbekistan, and both wild animals and domestic animals are important for maintaining their life cycle. Only one isolate from human sample was confirmed to be E. eqiinus (G4 genotype), which is known to be for the first time.
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Publication Date: 2020-04-30 PubMed ID: 32418392PubMed Central: PMC7231821DOI: 10.3347/kjp.2020.58.2.205Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study aims to identify the various types of the parasite Echinococcus granulosus present in Uzbekistan, a region with heavy livestock farming and a high occurrence of echinococcosis, an infection caused by this parasite.

Background

  • Echinococcus granulosus is a parasite that causes echinococcosis, an infection primarily affecting animals like sheep, cattle, goats, camels, and horses.
  • It is especially prevalent in countries with significant livestock farming industries like Central Asia, America, and Australia.
  • The parasite is known for having various genotypes that exhibit different characteristics and public health impacts.
  • Despite the high occurrence of echinococcosis in Uzbekistan, no prior studies investigated the specific genotypes of E. granulosus present in the region.

Methodology

  • The researchers conducted this study to identify the genotypes of Echinococcus that exist and are being transmitted in Uzbekistan.
  • They isolated Echinococcus from both definitive hosts (jackal and dog) and intermediate hosts (52 human isolates and 6 sheep isolates).
  • These isolates were then analyzed by sequencing two components of mitochondrial DNA: cox1 and nad1.

Results and Findings

  • All isolates except one were found to belong to the E. granulosus sensu stricto (s.s.) G1 and G3 genotypes.
  • The phylogenetic analysis based on the cox1 sequences showed that 42 human isolates, six sheep isolates, and one jackal isolate were of the G1 genotype.
  • The remaining eight human isolates and one dog isolate were of the G3 genotype.
  • The results suggest that the G1 and G3 genotypes of E. granulosus s.s. are predominant in Uzbekistan, indicating that wild and domestic animals are critical in maintaining the parasite’s life cycle.
  • One human isolate was found to be of the E. equinus (G4) genotype – a first-time observation and a crucial finding as this genotype has not been reported previously in humans in Uzbekistan.

Significance

  • This study provides valuable insights into the specific types of E. granulosus present in Uzbekistan, aiding further understanding of the echinococcosis outbreaks in the region.
  • It highlights the importance of both wild and domestic animals in the life cycle of this parasite, which could inform strategies to control and manage the disease in livestock and human populations.

Cite This Article

APA
Kim HJ, Yong TS, Shin MH, Lee KJ, Park GM, Suvonkulov U, Kovalenko D, Yu HS. (2020). Phylogenetic Characteristics of Echinococcus granulosus Sensu Lato in Uzbekistan. Korean J Parasitol, 58(2), 205-210. https://doi.org/10.3347/kjp.2020.58.2.205

Publication

The Korean journal of parasitology
ISSN: 1738-0006
NlmUniqueID: 9435800
Country: Korea (South)
Language: English
Volume: 58
Issue: 2
Pages: 205-210

Researcher Affiliations

Kim, Hye-Jin
  • Department of Parasitology and Tropical Medicine, Pusan National University, Yangsan 50612, Korea.
  • Biological Resources Research Group, Bioenvironmental Science & Toxicology Division, Gyeongnam Branch Institute, Korea Institute of Toxicology (KIT), Jinju 52834, Korea.
Yong, Tai-Soon
  • Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
Shin, Myeong Heon
  • Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 03722, Korea.
Lee, Kyu-Jae
  • Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine, Wonju 26426, Korea.
Park, Gab-Man
  • Department of Environmental Medical Biology, Catholic Kwandong University College of Medicine, Gangneung 25601, Korea.
Suvonkulov, Uktamjon
  • Isaev Research Institute of Medical Parasitology, Ministry of Health, Republic of Uzbekistan.
Kovalenko, Dmitriy
  • Isaev Research Institute of Medical Parasitology, Ministry of Health, Republic of Uzbekistan.
Yu, Hak Sun
  • Department of Parasitology and Tropical Medicine, Pusan National University, Yangsan 50612, Korea.
  • Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan 50612, Korea.

MeSH Terms

  • Animals
  • DNA, Mitochondrial / genetics
  • Echinococcosis / epidemiology
  • Echinococcosis / genetics
  • Echinococcus granulosus / genetics
  • Echinococcus granulosus / isolation & purification
  • Genotype
  • Humans
  • Phylogeny
  • Uzbekistan / epidemiology

Grant Funding

  • P2014-00076-1 / Korea International Cooperation Agency

Conflict of Interest Statement

. The authors declare no conflict of interest related to this study.

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Citations

This article has been cited 8 times.
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