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Home / Equine Research Bank / Virus Genes / Phylogenetic relationships of West Nile viruses isolated fro...
Virus genes2003; 26(2); 135-141; doi: 10.1023/a:1023431328933

Phylogenetic relationships of West Nile viruses isolated from birds and horses in Israel from 1997 to 2001.

Abstract: In November 1997, an outbreak of a neuroparalytic disease caused by West Nile (WN) virus was diagnosed in young goose flocks. Domestic geese were similarly affected in the late summer and fall of 1998, 1999, 2000 and 2001. WN viruses were also isolated from migratory and wild birds and horses in 1998-2001. A 1278 bp sequence of the envelope gene of 24 Israeli WN virus isolates was compared with those of seven isolates from Africa, Europe and New York. As a result, the Israeli isolates could then be grouped into two clusters. The 15 avian and three equine from 1997-2001 in the first cluster of viruses were shown to be identical to WN-NY99, while the second cluster comprised one goose isolate from 1998 and two goose and two pigeon isolates from 2000. These closely resembled the most recent Old World isolates, and indicate that at least two WN genotypes were co-circulating in the region during this time.
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Publication Date: 2003-06-14 PubMed ID: 12803465DOI: 10.1023/a:1023431328933Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on tracing the family tree of West Nile viruses found in birds and horses in Israel from 1997 to 2001, revealing at least two distinct genotypes.

Research Objective and Context

  • From 1997 to 2001, Israel suffered annual outbreaks of a neurological disease caused by the West Nile (WN) virus, primarily affecting young goose flocks. The WN virus was also found in migratory and wild birds as well as horses in the same timeframe.
  • The aim of the researchers was to determine the evolutionary relationships, also known as phylogenetic relationships, of the WN virus isolates collected during this period in order to understand the genetic variants and their origins.
  • An understanding of the phylogenetic relationships of a virus can assist in tracing the source of outbreak and planning effective preventive strategies.

Methodology

  • The researchers extracted a sequence of the envelope gene (a part of the virus genome) from 24 WN virus samples that were isolated in Israel across the specified timeframe.
  • This sequence, which is 1278 base pairs long, was compared with similar sequences from seven samples isolated in Africa, Europe, and New York.
  • The comparison of these sequences allowed the researchers to group the 24 Israeli isolates into two primary clusters based on their similarities and differences.

Key Findings

  • The first cluster, which included 15 bird and three horse isolates from 1997-2001, was found to be identical to the WN-NY99 strain seen in New York – suggesting a common ancestry.
  • The second cluster was made up of one goose isolate from 1998 and two each of goose and pigeon isolates from 2000. These closely resembled more recent isolates found in the Old World (Africa, Europe, and Asia), indicating they represented another distinct genotype of the WN virus.
  • These findings suggest that at least two distinct types of WN virus were circulating within bird and horse populations in Israel from 1997 to 2001. This has implications for pathogen surveillance, disease control measures, and vaccine development strategies.

Cite This Article

APA
Banet-Noach C, Malkinson M, Brill A, Samina I, Yadin H, Weisman Y, Pokamunski S, King R, Deubel V, Stram Y. (2003). Phylogenetic relationships of West Nile viruses isolated from birds and horses in Israel from 1997 to 2001. Virus Genes, 26(2), 135-141. https://doi.org/10.1023/a:1023431328933

Publication

Virus genes
ISSN: 0920-8569
NlmUniqueID: 8803967
Country: United States
Language: English
Volume: 26
Issue: 2
Pages: 135-141

Researcher Affiliations

Banet-Noach, Caroline
  • Kimron Veterinary Institute, Belt Dagan, Israel. carolinb@moag.gov.il
Malkinson, Mertyn
    Brill, Assaf
      Samina, Isak
        Yadin, Hagai
          Weisman, Yoram
            Pokamunski, Shimon
              King, Roni
                Deubel, Vincent
                  Stram, Yehuda

                    MeSH Terms

                    • Amino Acid Sequence
                    • Animals
                    • Birds / virology
                    • Disease Outbreaks / veterinary
                    • Genes, Viral
                    • Genotype
                    • Horses / virology
                    • Israel / epidemiology
                    • Molecular Sequence Data
                    • Phylogeny
                    • Sequence Homology, Amino Acid
                    • Time Factors
                    • Viral Proteins / genetics
                    • West Nile Fever / epidemiology
                    • West Nile Fever / veterinary
                    • West Nile Fever / virology
                    • West Nile virus / classification
                    • West Nile virus / genetics
                    • West Nile virus / isolation & purification

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