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Journal of animal physiology and animal nutrition2011; 96(4); 563-569; doi: 10.1111/j.1439-0396.2011.01179.x

Physiological and blood biochemical variables in horses exercising on a treadmill submerged in water.

Abstract: The blood lactate concentration (LA) and heart rate (HR) of 10 horses exercising in water on a treadmill were examined. With the water at 10 and 50% of the withers height (WH), the blood LA increased up to mean values around 1.9 mm during the standardized exercise test (SET) until after step 3 of 5 (each step lasted 5 min, speed increasing step by step). Thereafter, blood LA of horses remained constant, while with the water at 80% of WH, the blood LA decreased from the mean peak of 2.16 ± 0.62 mm after the 4th step. The HR of the horses increased to 132 ± 14 beats/min until the 3rd step of SET with the water at 10% of WH, up to the 2nd step with the water at 50% (134 ± 10 beats/min) and up to the 1st step only with the water at 80% of WH (134 ± 10 beats/min). In another SET, horses were exercised five times for 5 min at the maximal attainable speed of 5.5 m/s in water at 20% of the withers height in step 1, 35% in step 2, 49% in step 3, 63% in step 4 and 77% in step 5. On using this SET, blood LA increased to 1.91 ± 0.25 mm until after the 2nd step and decreased after the 3rd step. The HR increased between before commencing SET and the 1st step (143 ± 13 beats/min) and remained constant thereafter. In conclusion, increasing water height and speed of exercise does not augment continuously blood LA and HR of horses exercising in water treadmills.
Publication Date: 2011-06-21 PubMed ID: 21692872DOI: 10.1111/j.1439-0396.2011.01179.xGoogle Scholar: Lookup
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Summary

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This study evaluates how exercising in water of different depths impacts horses’ blood lactate concentrations and heart rates

Summary of research

The research examined the changes in blood lactate concentration, denoted as LA, and heart rate, HR, in 10 horses while they performed exercises on a water treadmill. The treadmill was placed in water at different heights relative to the horse’s withers height (WH), and the observations were made during these exercises.

Methodology

  • The exercises, called a standardized exercise test (SET), included five steps with each step lasting 5 minutes.
  • Within each step, the speed of the treadmill was gradually increased.
  • The water was set at 10%, 50% of the wither’s height, and at 80% of the wither’s height.
  • In a different experiment, the horses executed a SET at a max attainable speed of 5.5 m/s. In this scenario, the water was adjusted at 20%, 35%, 49%, 63%, and 77% of WH in sequential steps.
  • The increase in HR and LA were recorded before and after each step of the two SETs.

Results

  • When the water was at 10% and 50% of the WH, the blood LA increased and reached an average of around 1.9 mm until the completion of the third step of the SET. After this step, the blood LA remained constant for the remaining duration of the exercise.
  • But when the water was at 80% of WH, blood LA decreased from an average peak of 2.16 ± 0.62 mm after the completion of the fourth step of the exercise.
  • The HR rose to around 132 ± 14 beats/min until the third step of the SET with water at 10% WH, up to the second step with water at 50% (134 ± 10 beats/min), and up to the first step with water at 80% of WH (134 ± 10 beats/min).
  • In the experiment with a max speed of 5.5 m/s, blood LA, increased to 1.91 ± 0.25 mm until after the second step and then decreased after the third step. The HR increased between before commencing SET and the first step (143 ± 13 beats/min) and remained constant thereafter.

Conclusions

The study concluded that increasing the water level and intensifying the exercise speed didn’t consistently heighten the blood LA and HR of horses exercising on a water treadmill. By performing systematic and structured water treadmill exercises, the researchers discovered an observable physiological derivate in horses. The results suggest potential benefits, challenges, and overall health impacts that might be influenced by the different conditions of physical exercise.

Cite This Article

APA
Lindner A, Wäschle S, Sasse HH. (2011). Physiological and blood biochemical variables in horses exercising on a treadmill submerged in water. J Anim Physiol Anim Nutr (Berl), 96(4), 563-569. https://doi.org/10.1111/j.1439-0396.2011.01179.x

Publication

ISSN: 1439-0396
NlmUniqueID: 101126979
Country: Germany
Language: English
Volume: 96
Issue: 4
Pages: 563-569

Researcher Affiliations

Lindner, A
  • Arbeitsgruppe Pferd, 52428 Jülich, Germany. arnolindner@t-online.de
Wäschle, S
    Sasse, H H L

      MeSH Terms

      • Animals
      • Body Temperature
      • Exercise Test
      • Female
      • Horses / blood
      • Horses / physiology
      • Male
      • Physical Conditioning, Animal / physiology
      • Time Factors

      Citations

      This article has been cited 10 times.
      1. Sikorska U, Maśko M, Rey B, Domino M. Utility of Infrared Thermography for Monitoring of Surface Temperature Changes During Horses' Work on Water Treadmill with an Artificial River System. Animals (Basel) 2025 Aug 1;15(15).
        doi: 10.3390/ani15152266pubmed: 40805054google scholar: lookup
      2. Geiger T, Lindenhahn L, Delarocque J, Geburek F. Evaluation of water treadmill training, lunging and treadmill training in the rehabilitation of horses with back pain. BMC Vet Res 2025 Jul 29;21(1):495.
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        doi: 10.3390/ani12223086pubmed: 36428314google scholar: lookup
      6. Vitger AD, Bruhn-Rasmussen T, Pedersen EO, Fuglsang-Damgaard LH, Harrison AP. The impact of water depth and speed on muscle fiber activation of healthy dogs walking in a water treadmill. Acta Vet Scand 2021 Nov 24;63(1):46.
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        doi: 10.3390/ani11020305pubmed: 33530300google scholar: lookup
      9. Muñoz A, Saitua A, Becero M, Riber C, Satué K, de Medina AS, Argüelles D, Castejón-Riber C. The Use of the Water Treadmill for the Rehabilitation of Musculoskeletal Injuries in the Sport Horse. J Vet Res 2019 Sep;63(3):439-445.
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      10. Greco-Otto P, Bond S, Sides R, Kwong GPS, Bayly W, Léguillette R. Workload of horses on a water treadmill: effect of speed and water height on oxygen consumption and cardiorespiratory parameters. BMC Vet Res 2017 Nov 28;13(1):360.
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