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Veterinary research communications2007; 31(5); 591-601; doi: 10.1007/s11259-007-3518-2

Plasma concentrations of a type II collagen-derived peptide and its nitrated form in growing Ardenner sound horses and in horses suffering from juvenile digital degenerative osteoarthropathy.

Abstract: Several breeds of draft horses suffer from degenerative digital osteoarthropathy, resulting in a reduced active lifespan. A group of 30 Ardenner horses was followed, in standardized conditions, from 15 to 28 months of age to detect the early manifestations of the disease. The severity of the disease was assessed according to a personal grading system including clinical and radiographic items. Coll 2-1, a peptide of the helical region of type II collagen, and its nitrated form (Coll 2-1 NO(2)) were assayed in blood plasma collected at 452 +/- 18 days, 504 +/- 20 days, 558 +/- 18 days, 613 +/- 19 days, 675 +/- 19 days, 752 +/- 21 days and 852 +/- 19 days of age. At the end of the follow-up period, 53.3% of Ardenner horses were affected by a degenerative digital osteoarthropathy. A significant effect (p<0.05) of time, sex and pathology was observed for Coll 2-1 NO(2). Variations of Coll 2-1 were not significant except for the time effect. The elevation of Coll 2-1 NO(2) in the pathological group could indicate an inflammatory process during the growth of the affected horses, as nitration of tyrosine is mediated through reactive oxygen/nitrogen species and/or myeloperoxidase activity. Coll 2-1 NO(2) appears to be an interesting early marker of cartilage degradation and oxidation in degenerative osteoarthropathy.
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Publication Date: 2007-01-24 PubMed ID: 17252318DOI: 10.1007/s11259-007-3518-2Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the levels of a certain type of collagen-derived peptide in the blood of Ardenner draft horses. The researchers found that increased levels of this peptide were linked to a type of degenerative bone disease common in this breed.

Overview of the Study

  • The study was carried out on a group of 30 Ardenner horses, aged between 15 to 28 months. The aim was to identify early signs of degenerative digital osteoarthropathy, a disease common in several breeds of draft horses which shortens their active lifespan.
  • The severity of the disease in the horses was measured using a personalized grading system, including clinical and radiographic examinations. Blood plasma was also collected from the horses at specified intervals, to measure levels of Coll 2-1, a type II collagen-derived peptide, and its nitrated form (Coll 2-1 NO(2)).

The Findings

  • By the end of the monitoring period, slightly over half (53.3%) of the horses were found to be affected by the disease.
  • Significant changes in levels of Coll 2-1 NO(2) were observed over time, with differences also noted based on sex and presence of the disease.
  • The only significant variations observed in Coll 2-1 levels were related to the passage of time.

Analysis and Implications

  • The increase in Coll 2-1 NO(2) levels in affected horses suggests that an inflammatory process is occurring during growth. It is postulated that this inflammation could be due to nitration of tyrosine, a process mediated by reactive oxygen/nitrogen species and/or the activity of an enzyme called myeloperoxidase.
  • In light of these findings, Coll 2-1 NO(2) appears to be a potentially useful early marker for cartilage degradation and oxidation in degenerative osteoarthropathy. This could be instrumental in early diagnosis, management, and treatment of this debilitative disease in horses.

Cite This Article

APA
Lejeune JP, Serteyn D, Gangl M, Schneider N, Deby-Dupont G, Deberg M, Henrotin Y. (2007). Plasma concentrations of a type II collagen-derived peptide and its nitrated form in growing Ardenner sound horses and in horses suffering from juvenile digital degenerative osteoarthropathy. Vet Res Commun, 31(5), 591-601. https://doi.org/10.1007/s11259-007-3518-2

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 31
Issue: 5
Pages: 591-601

Researcher Affiliations

Lejeune, J-Ph
  • Centre Européen du Cheval, Mont-le-Soie, Vielsalm, Belgium. jph.lejeune@student.ulg.ac.be
Serteyn, D
    Gangl, M
      Schneider, N
        Deby-Dupont, G
          Deberg, M
            Henrotin, Y

              MeSH Terms

              • Aging
              • Animals
              • Bone Diseases / blood
              • Bone Diseases / pathology
              • Bone Diseases / veterinary
              • Collagen Type II / blood
              • Collagen Type II / chemistry
              • Female
              • Health
              • Horse Diseases / blood
              • Horse Diseases / pathology
              • Horses / blood
              • Horses / growth & development
              • Joint Diseases / blood
              • Joint Diseases / pathology
              • Joint Diseases / veterinary
              • Male
              • Sex Characteristics

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              Citations

              This article has been cited 2 times.
              1. Turlo AJ, Cywinska A, Frisbie DD. Revisiting predictive biomarkers of musculoskeletal injury in thoroughbred racehorses: longitudinal study in polish population. BMC Vet Res 2019 Feb 26;15(1):66.
                doi: 10.1186/s12917-019-1799-7pubmed: 30808359google scholar: lookup
              2. Verwilghen DR, Martens A, Busschers E, Franck T, Deberg M, Henrotin Y, Vanderheyden L, Serteyn D. Coll2-1, Coll2-1NO2 and myeloperoxidase concentrations in the synovial fluid of equine tarsocrural joints affected with osteochondrosis. Vet Res Commun 2011 Oct;35(7):401-8.
                doi: 10.1007/s11259-011-9487-5pubmed: 21681550google scholar: lookup
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