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Home / Equine Research Bank / BMC Vet Res / Plasma proteomics shows an elevation of the anti-inflammator...
BMC veterinary research2012; 8; 179; doi: 10.1186/1746-6148-8-179

Plasma proteomics shows an elevation of the anti-inflammatory protein APOA-IV in chronic equine laminitis.

Abstract: Equine laminitis is a devastating disease that causes severe pain in afflicted horses and places a major economic burden on the horse industry. In acute laminitis, the disintegration of the dermal-epidermal junction can cause the third phalanx to detach from the hoof wall, leaving the horse unable to bear weight on the affected limbs. Horses that survive the acute phase transition into a chronic form of laminitis, which is often termed "founder". Some evidence suggests that chronic laminar inflammation might be associated with alterations in the endocrine and immune systems. We investigated this broad hypothesis by using DIGE to assess global differences in the plasma proteome between horses with chronic laminitis and controls. Results: We identified 16 differentially expressed proteins; the majority of these were involved in the interrelated coagulation, clotting, and kininogen cascades. Clinical testing of functional coagulation parameters in foundered horses revealed a slight delay in prothrombin (PT) clotting time, although most other indices were within normal ranges. Upregulation of the intestinal apolipoprotein APOA-IV in horses with chronic laminitis was confirmed by western blot. Conclusions: Our results support the hypothesis that localized laminar inflammation may be linked to systemic alterations in immune regulation, particularly in the gastrointestinal system. Gastrointestinal inflammation has been implicated in the development of acute laminitis but has not previously been associated with chronic laminitis.
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Publication Date: 2012-09-27 PubMed ID: 23016951PubMed Central: PMC3511297DOI: 10.1186/1746-6148-8-179Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examined the blood plasma of horses suffering from chronic laminitis and discovered an increase in anti-inflammatory protein APOA-IV and differences in protein levels connected to coagulation and clotting activities.

Overview of Study and Purpose

  • The research focused on equine laminitis, a serious disease affecting horses that is characterized by severe pain and can cause major financial costs in the horse industry. Laminitis causes a breakdown in the connection between the third phalanx of a horse’s leg and the hoof wall. The horses experiencing this disease often cannot bear weight on the impacted limbs.
  • The study sought to understand whether chronic laminitis is linked to changes in the endocrine and immune systems, possibly bringing about chronic inflammation. This concept was explored by comparatively analyzing the plasma proteome of healthy horses and those suffering from chronic laminitis.

Study Findings

  • The researchers discovered 16 proteins that were expressed differently between horses with and without chronic laminitis. Most of these alterations involved proteins that have a role in coagulation, clotting, and kininogen pathways.
  • When functional coagulation metrics were observed in laminitis-infected horses, the study showed a minor delay in prothrombin clotting time; however, all other analyzed indices were within typical ranges.
  • An increase in the apolipoprotein APOA-IV—an anti-inflammatory protein found in the intestines—was observed in horses dealing with chronic laminitis. This finding was substantiated using western blot, a lab technique used to detect specific protein levels in a sample.

Study Conclusion

  • The study’s findings support the earlier hypothesis that localized inflammation associated with chronic laminitis may be linked to changes occurring in systemic immune regulation. This is most notably seen in the observed alterations in the gastrointestinal system.
  • The study results suggest a novel association between chronic laminitis and gastrointestinal inflammation. Notably, the latter has been previously implicated in the development of acute laminitis, but until now, has not been tied to the disease’s chronic form.

Cite This Article

APA
Steelman SM, Chowdhary BP. (2012). Plasma proteomics shows an elevation of the anti-inflammatory protein APOA-IV in chronic equine laminitis. BMC Vet Res, 8, 179. https://doi.org/10.1186/1746-6148-8-179

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 8
Pages: 179

Researcher Affiliations

Steelman, Samantha M
  • Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77845-4458, USA.
Chowdhary, Bhanu P

    MeSH Terms

    • Animals
    • Apolipoproteins A / blood
    • Apolipoproteins A / genetics
    • Apolipoproteins A / metabolism
    • Blood Coagulation
    • Chronic Disease
    • Female
    • Foot Diseases / metabolism
    • Foot Diseases / veterinary
    • Gene Expression Regulation / physiology
    • Hoof and Claw / metabolism
    • Hoof and Claw / pathology
    • Horse Diseases / metabolism
    • Horse Diseases / pathology
    • Horses
    • Inflammation / metabolism
    • Inflammation / veterinary
    • Male
    • Proteomics
    • Transcriptome

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