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Home / Equine Research Bank / PLoS One / Plasmid Profiles of Virulent Rhodococcus equi Strains Isolat...
PloS one2016; 11(4); e0152887; doi: 10.1371/journal.pone.0152887

Plasmid Profiles of Virulent Rhodococcus equi Strains Isolated from Infected Foals in Poland.

Abstract: Rhodococcus equi is an important bacterial pathogen in foals up to 6 months old, widespread in horse farms all over the world. It was found that only virulent R. equi strains expressing 15-17 kDa virulence-associated protein (VapA) and having large virulence plasmid of 85-90 kb containing vapA gene are pathogenic for horses. To date, 12 plasmid types have been reported in VapA positive strains from horses. There are no data concerning plasmid types of Polish field R. equi strains isolated from horses and horse farm environment. The aim of the study is to determine plasmid profiles of virulent R. equi strains isolated in Poland from dead foals as well as from soil samples taken from horse breeding farms. Plasmid profiles of 10 clinical strains derived from 8 farms and 11 environmental strains from 3 farms, confirmed as virulent by PCR, were compared with 12 reference strains containing the known plasmid size and type. Plasmid DNAs were analysed by digestion with the restriction endonucleases BamHI, EcoRI, EcoT22I, and HindIII for detailed comparison and estimation of plasmid sizes. The results of RFLP analysis revealed that all except one isolates used in the study are classified as VapA 85 kb type I plasmid. One strain harboured VapA 87 kb type I plasmid. This is the first report of plasmid types of Polish field R. equi strains. The results of our preliminary investigations on horse farms located in central and eastern Poland indicate that the virulent R. equi strains thus far isolated from diseased foals and horse farms environment represent a highly uniform plasmid pattern.
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Publication Date: 2016-04-13 PubMed ID: 27074033PubMed Central: PMC4830601DOI: 10.1371/journal.pone.0152887Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research observes the plasmid profiles of a harmful bacteria called Rhodococcus equi found in foals in Poland. The study found that the majority of the bacteria carry a specific type of plasmid, which plays a crucial role in the bacteria’s virulence.

Introduction to Rhodococcus equi

  • Rhodococcus equi is a dangerous bacterial pathogen that affects foals less than 6 months old.
  • The bacteria is commonplace in horse farms worldwide.
  • It is specifically pathogenic to horses if it expresses 15-17 kDa virulence-associated protein (VapA) and carries a large virulence plasmid (small, circular, double-stranded DNA molecule) of 85-90 kb that holds the vapA gene.

Objective of the Research

  • The study seeks to establish the varying plasmid profiles of virulent R. equi strains that were isolated in Poland from deceased foals and from soil samples collected from horse breeding farms.
  • This is because there is no existing data relating to the plasmid types of Polish field R. equi strains extracted from horses and their environments.

Methodology

  • Researchers assembled the plasmid profiles of 10 clinical strains from 8 farms. They also studied 11 environmental strains from 3 other farms.
  • These were all validated as virulent through the Polymerase Chain Reaction (PCR) method.
  • The strains were then compared with 12 reference strains that were known to contain specific plasmid size and type.
  • The plasmid DNA was studied using the restriction endonucleases (enzymes that cut DNA at specific sites) BamHI, EcoRI, EcoT22I, and HindIII to estimate the plasmid sizes and elicit a more detailed comparison.

Results

  • The Restriction Fragment Length Polymorphism (RFLP) analysis indicated that almost all the isolates used in the test were classified as the VapA 85 kb type I plasmid. There was an exception in one strain, which carried the VapA 87 kb type I plasmid.
  • This was the first case of identifying the plasmid types of Polish field R. equi strains.
  • The preliminary results show that the virulent R. equi strains thus far isolated from diseased foals and horse farms environment across central and eastern Poland were found to represent a highly uniform plasmid pattern.

Cite This Article

APA
Kalinowski M, Grądzki Z, Jarosz Ł, Kato K, Hieda Y, Kakuda T, Takai S. (2016). Plasmid Profiles of Virulent Rhodococcus equi Strains Isolated from Infected Foals in Poland. PLoS One, 11(4), e0152887. https://doi.org/10.1371/journal.pone.0152887

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 11
Issue: 4
Pages: e0152887
PII: e0152887

Researcher Affiliations

Kalinowski, Marcin
  • Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Grądzki, Zbigniew
  • Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Jarosz, Łukasz
  • Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Kato, Kiyoko
  • School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori, Japan.
Hieda, Yu
  • School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori, Japan.
Kakuda, Tsutomu
  • School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori, Japan.
Takai, Shinji
  • School of Veterinary Medicine and Animal Science, Kitasato University, Towada, Aomori, Japan.

MeSH Terms

  • Actinomycetales Infections / microbiology
  • Animals
  • DNA, Bacterial
  • Horse Diseases / microbiology
  • Horses
  • Plasmids / genetics
  • Plasmids / metabolism
  • Poland
  • Polymerase Chain Reaction / veterinary
  • Rhodococcus equi / isolation & purification
  • Virulence Factors / genetics

Conflict of Interest Statement

The authors have declared that no competing interests exist.

References

This article includes 32 references
  1. Giguère S, Prescott JF. Clinical manifestations, diagnosis, treatment, and prevention of Rhodococcus equi infections in foals.. Vet Microbiol 1997 Jun 16;56(3-4):313-34.
    pubmed: 9226845doi: 10.1016/s0378-1135(97)00099-0google scholar: lookup
  2. Meijer WG, Prescott JF. Rhodococcus equi.. Vet Res 2004 Jul-Aug;35(4):383-96.
    pubmed: 15236672doi: 10.1051/vetres:2004024google scholar: lookup
  3. Cohen ND. Rhodococcus equi foal pneumonia.. Vet Clin North Am Equine Pract 2014 Dec;30(3):609-22.
    doi: 10.1016/j.cveq.2014.08.010pubmed: 25282322google scholar: lookup
  4. Takai S. Epidemiology of Rhodococcus equi infections: a review.. Vet Microbiol 1997 Jun 16;56(3-4):167-76.
    pubmed: 9226831doi: 10.1016/s0378-1135(97)00085-0google scholar: lookup
  5. Giguère S, Cohen ND, Chaffin MK, Hines SA, Hondalus MK, Prescott JF, Slovis NM. Rhodococcus equi: clinical manifestations, virulence, and immunity.. J Vet Intern Med 2011 Nov-Dec;25(6):1221-30.
    doi: 10.1111/j.1939-1676.2011.00804.xpubmed: 22092609google scholar: lookup
  6. Reuss SM, Cohen ND. Update on bacterial pneumonia in the foal and weanling.. Vet Clin North Am Equine Pract 2015 Apr;31(1):121-35.
    doi: 10.1016/j.cveq.2014.11.004pubmed: 25600452google scholar: lookup
  7. Grądzki Z, Ziętek A, Hetman E, Winiarczyk S. Występowanie szczepów Rhodococcus equi w glebie w stadninach z występującą rodokokozą. Med Weter 2006;62: 1310–1312.
  8. Muscatello G, Leadon DP, Klayt M, Ocampo-Sosa A, Lewis DA, Fogarty U, Buckley T, Gilkerson JR, Meijer WG, Vazquez-Boland JA. Rhodococcus equi infection in foals: the science of 'rattles'.. Equine Vet J 2007 Sep;39(5):470-8.
    pubmed: 17910275doi: 10.2746/042516407x209217google scholar: lookup
  9. Venner M, Meyer-Hamme B, Verspohl J, Hatori F, Shimizu N, Sasaki Y, Kakuda T, Tsubaki S, Takai S. Genotypic characterization of VapA positive Rhodococcus equi in foals with pulmonary affection and their soil environment on a warmblood horse breeding farm in Germany.. Res Vet Sci 2007 Dec;83(3):311-7.
    pubmed: 17360011doi: 10.1016/j.rvsc.2007.01.009google scholar: lookup
  10. Takai S, Tharavichitkul P, Takarn P, Khantawa B, Tamura M, Tsukamoto A, Takayama S, Yamatoda N, Kimura A, Sasaki Y, Kakuda T, Tsubaki S, Maneekarn N, Sirisanthana T, Kirikae T. Molecular epidemiology of Rhodococcus equi of intermediate virulence isolated from patients with and without acquired immune deficiency syndrome in Chiang Mai, Thailand.. J Infect Dis 2003 Dec 1;188(11):1717-23.
    pubmed: 14639543doi: 10.1086/379739google scholar: lookup
  11. Topino S, Galati V, Grilli E, Petrosillo N. Rhodococcus equi infection in HIV-infected individuals: case reports and review of the literature.. AIDS Patient Care STDS 2010 Apr;24(4):211-22.
    doi: 10.1089/apc.2009.0248pubmed: 20377432google scholar: lookup
  12. Takai S, Sekizaki T, Ozawa T, Sugawara T, Watanabe Y, Tsubaki S. Association between a large plasmid and 15- to 17-kilodalton antigens in virulent Rhodococcus equi.. Infect Immun 1991 Nov;59(11):4056-60.
    pmc: PMC258996pubmed: 1937765doi: 10.1128/iai.59.11.4056-4060.1991google scholar: lookup
  13. Takai S, Ohbushi S, Koike K, Tsubaki S, Oishi H, Kamada M. Prevalence of virulent Rhodococcus equi in isolates from soil and feces of horses from horse-breeding farms with and without endemic infections.. J Clin Microbiol 1991 Dec;29(12):2887-9.
    pmc: PMC270455pubmed: 1757567doi: 10.1128/jcm.29.12.2887-2889.1991google scholar: lookup
  14. Hondalus MK. Pathogenesis and virulence of Rhodococcus equi.. Vet Microbiol 1997 Jun 16;56(3-4):257-68.
    pubmed: 9226840doi: 10.1016/s0378-1135(97)00094-1google scholar: lookup
  15. Muscatello G. Rhodococcus equi pneumonia in the foal--part 1: pathogenesis and epidemiology.. Vet J 2012 Apr;192(1):20-6.
    doi: 10.1016/j.tvjl.2011.08.014pubmed: 22015138google scholar: lookup
  16. Vázquez-Boland JA, Giguère S, Hapeshi A, MacArthur I, Anastasi E, Valero-Rello A. Rhodococcus equi: the many facets of a pathogenic actinomycete.. Vet Microbiol 2013 Nov 29;167(1-2):9-33.
    doi: 10.1016/j.vetmic.2013.06.016pubmed: 23993705google scholar: lookup
  17. Son WG, Lee DS, Yamatoda N, Hatori F, Shimizu N, Kakuda T, Sasaki Y, Tsubaki S, Takai S. Molecular typing of VapA-positive Rhodococcus equi isolates from Jeju native horses, Korea.. J Vet Med Sci 2006 Mar;68(3):249-53.
    pubmed: 16598168doi: 10.1292/jvms.68.249google scholar: lookup
  18. Duquesne F, Hébert L, Sévin C, Breuil MF, Tapprest J, Laugier C, Petry S. Analysis of plasmid diversity in 96 Rhodococcus equi strains isolated in Normandy (France) and sequencing of the 87-kb type I virulence plasmid.. FEMS Microbiol Lett 2010 Oct;311(1):76-81.
    doi: 10.1111/j.1574-6968.2010.02070.xpubmed: 20707817google scholar: lookup
  19. Takai S, Chaffin MK, Cohen ND, Hara M, Nakamura M, Kakuda T, Sasaki Y, Tsubaki S, Martens RJ. Prevalence of virulent Rhodococcus equi in soil from five R. equi-endemic horse-breeding farms and restriction fragment length polymorphisms of virulence plasmids in isolates from soil and infected foals in Texas.. J Vet Diagn Invest 2001 Nov;13(6):489-94.
    pubmed: 11724139doi: 10.1177/104063870101300606google scholar: lookup
  20. Takai S, Shoda M, Sasaki Y, Tsubaki S, Fortier G, Pronost S, Rahal K, Becu T, Begg A, Browning G, Nicholson VM, Prescott JF. Restriction fragment length polymorphisms of virulence plasmids in Rhodococcus equi.. J Clin Microbiol 1999 Oct;37(10):3417-20.
    pmc: PMC85590pubmed: 10488224doi: 10.1128/jcm.37.10.3417-3420.1999google scholar: lookup
  21. Takai S, Son WG, Lee DS, Madarame H, Seki I, Yamatoda N, Kimura A, Kakuda T, Sasaki Y, Tsubaki S, Lim YK. Rhodococcus equi virulence plasmids recovered from horses and their environment in Jeju, Korea: 90-kb type II and a new variant, 90-kb type V.. J Vet Med Sci 2003 Dec;65(12):1313-7.
    pubmed: 14709819doi: 10.1292/jvms.65.1313google scholar: lookup
  22. Makrai L, Takai S, Tamura M, Tsukamoto A, Sekimoto R, Sasaki Y, Kakuda T, Tsubaki S, Varga J, Fodor L, Solymosi N, Major A. Characterization of virulence plasmid types in Rhodococcus equi isolates from foals, pigs, humans and soil in Hungary.. Vet Microbiol 2002 Sep 24;88(4):377-84.
    pubmed: 12220812doi: 10.1016/s0378-1135(02)00157-8google scholar: lookup
  23. Makrai L, Kobayashi A, Matsuoka M, Sasaki Y, Kakuda T, Dénes B, Hajtós I, Révész I, Jánosi K, Fodor L, Varga J, Takai S. Isolation and characterisation of Rhodococcus equi from submaxillary lymph nodes of wild boars (Sus scrofa).. Vet Microbiol 2008 Oct 15;131(3-4):318-23.
    doi: 10.1016/j.vetmic.2008.04.009pubmed: 18499361google scholar: lookup
  24. Woolcock JB, Farmer AM, Mutimer MD. Selective medium for Corynebacterium equi isolation.. J Clin Microbiol 1979 May;9(5):640-2.
    pmc: PMC275365pubmed: 479362doi: 10.1128/jcm.9.5.640-642.1979google scholar: lookup
  25. Sellon DC, Walker K, Suyemoto M, Altier C. Nucleic acid amplification for rapid detection of Rhodococcus equi in equine blood and tracheal wash fluids.. Am J Vet Res 1997 Nov;58(11):1232-7.
    pubmed: 9361884
  26. Ziętek-Barszcz A, Grądzki Z. Optimisation of the DNA extraction method from foal faeces for the diagnosis of Rhodococcus equi infections using PCR. Bull Vet Inst Pulawy 2009;53: 345–349.
  27. Bell KS, Philp JC, Christofi N, Aw DW. Identification of Rhodococcus equi using the polymerase chain reaction.. Lett Appl Microbiol 1996 Aug;23(2):72-4.
    pubmed: 8987445doi: 10.1111/j.1472-765x.1996.tb00033.xgoogle scholar: lookup
  28. Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Res 1979 Nov 24;7(6):1513-23.
    pmc: PMC342324pubmed: 388356doi: 10.1093/nar/7.6.1513google scholar: lookup
  29. Takai S, Watanabe Y, Ikeda T, Ozawa T, Matsukura S, Tamada Y, Tsubaki S, Sekizaki T. Virulence-associated plasmids in Rhodococcus equi.. J Clin Microbiol 1993 Jul;31(7):1726-9.
    pmc: PMC265621pubmed: 8349748doi: 10.1128/jcm.31.7.1726-1729.1993google scholar: lookup
  30. Nicholson VM, Prescott JF. Restriction enzyme analysis of the virulence plasmids of VapA-positive Rhodococcus equi strains isolated from humans and horses.. J Clin Microbiol 1997 Mar;35(3):738-40.
    pmc: PMC229662pubmed: 9041424doi: 10.1128/jcm.35.3.738-740.1997google scholar: lookup
  31. Ribeiro MG, Seki I, Yasuoka K, Kakuda T, Sasaki Y, Tsubaki S, Takai S. Molecular epidemiology of virulent Rhodococcus equi from foals in Brazil: virulence plasmids of 85-kb type I, 87-kb type I, and a new variant, 87-kb type III.. Comp Immunol Microbiol Infect Dis 2005 Jan;28(1):53-61.
    pubmed: 15563953doi: 10.1016/j.cimid.2004.07.001google scholar: lookup
  32. Takai S, Sengee S, Madarame H, Hatori F, Yasuoka K, Ochirel E, Sasaki Y, Kakuda T, Tsubaki S, Bandi N, Sodnomdarjaa R. The absence of Rhodococcus equi in Mongolian horses.. J Vet Med Sci 2005 Jun;67(6):611-3.
    pubmed: 15997190doi: 10.1292/jvms.67.611google scholar: lookup

Citations

This article has been cited 4 times.
  1. Kalinowski M, Grądzki Z, Jarosz Ł, Adaszek Ł. Molecular analysis of the chromosomal 16S rRNA gene and vapA plasmid gene of Polish field strains of R. equi. PLoS One 2018;13(9):e0204024.
    doi: 10.1371/journal.pone.0204024pubmed: 30252885google scholar: lookup
  2. Yerlikaya Z, Karagülle B, Otlu B, Muz A. From Paddock to Foal: Prevalence and Genotypic Diversity of Rhodococcus equi on Stud Farms in Türkiye. Vet Sci 2026 Jan 10;13(1).
    doi: 10.3390/vetsci13010072pubmed: 41600728google scholar: lookup
  3. Ghielmetti G, Stevens MJA, Schmitt S, Kittl S, Cernela N, Biggel M, Schulthess B, Keller PM, Schrenzel J, Stephan R. Multi-host distribution of Rhodococcus equi (Prescottella equi) strains and their phylogenomic clustering. BMC Microbiol 2025 Jul 21;25(1):447.
    doi: 10.1186/s12866-025-04152-8pubmed: 40691552google scholar: lookup
  4. da Silveira BP, Cohen ND, Lawhon SD, Watson RO, Bordin AI. Protective immune response against Rhodococcus equi: An innate immunity-focused review. Equine Vet J 2025 May;57(3):563-586.
    doi: 10.1111/evj.14214pubmed: 39258739google scholar: lookup
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