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Veterinary research communications1988; 12(2-3); 101-107; doi: 10.1007/BF00362788

Platelet activating factor as a mediator of equine cell locomotion.

Abstract: Equine polymorphonuclear (PMN) and mononuclear (MN) leucocytes were separated on Percoll gradients and used to study the chemoattractant properties of the polar ether-linked phospholipid, platelet activating factor (PAF). Six concentrations of PAF ranging from 1 ng/ml to 100 micrograms/ml were studied in each of two in vitro assay systems, the agarose microdroplet and a microfilter technique. Very significant (p less than 0.01) increases in the movement of both PMN and MN cells were obtained with most concentrations of PAF. In two instances there was no apparent concentration-response relationship, although the action of PAF was approximately bell-shaped in two others. The possible significance of these findings for equine inflammatory conditions is discussed.
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Publication Date: 1988-01-01 PubMed ID: 3188378DOI: 10.1007/BF00362788Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the effects of the phospholipid, platelet activating factor (PAF), on the movement of two types of equine white blood cells. Increased cell movement was observed with most concentrations of PAF, which may have implications for understanding equine inflammatory conditions.

Objective of the Research

The researchers aimed to study the influence of platelet activating factor (PAF) on the locomotion of two types of equine white blood cells, polymorphonuclear (PMN) and mononuclear (MN) leucocytes. The purpose of this study was to advance the understanding of equine cell movement, with a particular focus on the inflammatory response.

Methodology

  • The research team isolated PMN and MN leucocytes from horses, employing Percoll gradients for cell separation.
  • These cells were then exposed to six distinct concentrations of PAF, ranging from 1 nanogram per milliliter to 100 micrograms per milliliter.
  • The effects of PAF on cell locomotion were evaluated using two in vitro assay systems: the agarose microdroplet method and a microfilter technique.

Findings

  • Significant increases in cell movement were observed in both PMN and MN leucocytes across most PAF concentrations, indicating PAF’s potency as a chemoattractant.
  • Not all lab results demonstrated a concentration-response relationship; the influence of PAF on cell locomotion did not consistently increase with concentration.
  • In two instances, the influence of PAF on cell movement followed a bell-shaped curve; the degree of cell locomotion initially increased with concentration, peaked, then decreased.

Implications

These findings potentially carry significance for understanding equine inflammatory conditions, as the movement of leukocytes is essential for the inflammatory response. Increased understanding about the role of PAF in promoting cell movement may further inform veterinarians and equine scientists about how to manage and treat conditions involving inflammation. However, the researchers mention that the relationship between PAF concentration and cell locomotion is not straightforward, and additional investigation is required.

Cite This Article

APA
Dawson J, Lees P, Sedgwick AD. (1988). Platelet activating factor as a mediator of equine cell locomotion. Vet Res Commun, 12(2-3), 101-107. https://doi.org/10.1007/BF00362788

Publication

Veterinary research communications
ISSN: 0165-7380
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 12
Issue: 2-3
Pages: 101-107

Researcher Affiliations

Dawson, J
  • Royal Veterinary College, University of London, Great Britain.
Lees, P
    Sedgwick, A D

      MeSH Terms

      • Animals
      • Chemotaxis, Leukocyte
      • Horses / blood
      • Leukocytes, Mononuclear / physiology
      • Neutrophils / physiology
      • Platelet Activating Factor / pharmacology

      References

      This article includes 19 references
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      Citations

      This article has been cited 1 times.
      1. McEwen BJ, Wilcock BP, Eyre P. The effect of leukotriene B4, leukotriene C4, zymosan activated serum, histamine, tabanid extract and N-formyl-methionyl-leucyl-phenylalanine on the in vitro migration of equine eosinophils. Can J Vet Res 1990 Oct;54(4):400-4.
        pubmed: 2174292
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