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Animals : an open access journal from MDPI2022; 12(2); 189; doi: 10.3390/ani12020189

Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same.

Abstract: Platelet lysate (PL) is an attractive platelet-based therapeutic tool and has shown promise as xeno-free replacement for fetal bovine serum (FBS) in human and equine mesenchymal stromal cell (MSC) culture. Here, we established a scalable buffy-coat-based protocol for canine PL (cPL) production (n = 12). The cPL was tested in canine adipose MSC (n = 5) culture compared to FBS. For further comparison, equine adipose MSC (n = 5) were cultured with analogous equine PL (ePL) or FBS. During canine blood processing, platelet and transforming growth factor-β1 concentrations increased ( < 0.05 and < 0.001), while white blood cell concentrations decreased ( < 0.05). However, while equine MSC showed good results when cultured with 10% ePL, canine MSC cultured with 2.5% or 10% cPL changed their morphology and showed decreased metabolic activity ( < 0.05). Apoptosis and necrosis in canine MSC were increased with 2.5% cPL ( < 0.05). Surprisingly, passage 5 canine MSC showed less genetic aberrations after culture with 10% cPL than with FBS. Our data reveal that using analogous canine and equine biologicals does not entail the same results. The buffy-coat-based cPL was not adequate for canine MSC culture, but may still be useful for therapeutic applications.
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Publication Date: 2022-01-13 PubMed ID: 35049811PubMed Central: PMC8773277DOI: 10.3390/ani12020189Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper explores the use of platelet lysate, a platelet-derived therapy, as a potential non-animal serum substitute in the culture of mesenchymal stromal cells in dogs and horses. Results indicate variable success with the method, creating a difference in cellular effects between the two species.

Study Purpose and Methodology

  • The study aimed to understand the effectiveness of platelet lysate (PL) as a replacement for fetal bovine serum (FBS), a commonly used supplement in cell culture in both equine and canine mesenchymal stromal cell (MSC) cultures. PL has shown promise as a replacement for animal serums in cellular culture and could eliminate the risks associated with animal serum use.
  • Researchers set up a scalable protocol for canine platelet lysate (cPL) production using the ‘buffy coat’ method, taking blood samples from 12 dogs. This process separates blood components, allowing for concentrated extraction of platelets.
  • They compared the effects of cPL and FBS on canine adipose MSC cultures from five dog samples and on equine adipose MSC cultures from five horse samples using equine PL (ePL).

Results

  • There were changes in the concentration of platelets and transforming growth factor-β1 (a protein that helps cells grow and divide) in canine blood during processing for PL.
  • Equine MSCs exhibited positive growth when cultured with ePL, whereas canine MSCs showed changes in cell shape, reduced metabolic activity, and increased cell death when cultured with cPL.
  • Canine MSCs displayed decreased instances of genetic abnormalities when cultured with cPL as compared to FBS-cultured cells, suggesting potential benefits of cPL despite initial challenges.

Synthesis and Implications

  • The study revealed that using the same biological material, in this case, PL, doesn’t yield the same results in different species, emphasizing the need for species-specific research in the context of cell culture supplements.
  • The results suggest that while cPL may not be optimal for canine MSC culture due to changes in cell morphology and increased cell death, cPL could still prove to be a useful tool in therapeutic applications.

Cite This Article

APA
Hagen A, Holland H, Brandt VP, Doll CU, Häußler TC, Melzer M, Moellerberndt J, Lehmann H, Burk J. (2022). Platelet Lysate for Mesenchymal Stromal Cell Culture in the Canine and Equine Species: Analogous but Not the Same. Animals (Basel), 12(2), 189. https://doi.org/10.3390/ani12020189

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 2
PII: 189

Researcher Affiliations

Hagen, Alina
  • Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, 35390 Giessen, Germany.
Holland, Heidrun
  • Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, 04109 Leipzig, Germany.
Brandt, Vivian-Pascal
  • Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, 04109 Leipzig, Germany.
Doll, Carla U
  • Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, 35390 Giessen, Germany.
Häußler, Thomas C
  • Small Animal Clinic (Surgery), Justus-Liebig-University Giessen, 35390 Giessen, Germany.
Melzer, Michaela
  • Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, 35390 Giessen, Germany.
Moellerberndt, Julia
  • Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, 35390 Giessen, Germany.
Lehmann, Hendrik
  • Department of Veterinary Clinical Sciences, Small Animal Clinic, Justus-Liebig-University Giessen, 35390 Giessen, Germany.
Burk, Janina
  • Equine Clinic (Surgery, Orthopedics), Justus-Liebig-University Giessen, 35390 Giessen, Germany.

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Citations

This article has been cited 4 times.
  1. Rashid U, Saba E, Yousaf A, Tareen WA, Sarfraz A, Rhee MH, Sandhu MA. Autologous Platelet Lysate Is an Alternative to Fetal Bovine Serum for Canine Adipose-Derived Mesenchymal Stem Cell Culture and Differentiation.. Animals (Basel) 2023 Aug 17;13(16).
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  2. Iacono E, Merlo B. Stem Cells in Domestic Animals: Applications in Health and Production.. Animals (Basel) 2022 Oct 13;12(20).
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  3. Hagen A, Niebert S, Brandt VP, Holland H, Melzer M, Wehrend A, Burk J. Functional properties of equine adipose-derived mesenchymal stromal cells cultured with equine platelet lysate.. Front Vet Sci 2022;9:890302.
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  4. Pilgrim CR, McCahill KA, Rops JG, Dufour JM, Russell KA, Koch TG. A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine.. Front Vet Sci 2022;9:859025.
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