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Experimental and molecular pathology2003; 74(3); 244-255; doi: 10.1016/s0014-4800(03)00017-0

Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing.

Abstract: Nonhealing wounds of the lower equine limb represent a challenging model. The platelet is a natural source of a myriad of growth factors and cytokines that promote wound healing. This study evaluates the potential of platelet derived factors to enhance wound healing in the lower equine limb. Platelets were isolated from horse blood and activated with thrombin, a process known to induce growth factor release. This produced a platelet gel composed of platelet-rich plasma (PRP). To test this all-natural wound healant, 2.5-cm(2) full thickness cutaneous wounds were created below the knee and hock of a thoroughbred horse. Wounds were treated with PRP gel or left untreated. Sequential wound biopsies collected at Days 7, 36, and 79 postwounding permitted comparison of the temporal expression of differentiation markers and wound repair. To test the hypothesis that wounds treated with PRP gel exhibit more rapid epithelial differentiation and enhanced organization of dermal collagen compared to controls, tissues were stained for cytokeratin 10, a suprabasal differentiation marker, and the reestablishment of collagen was evaluated by trichrome staining. PRP gel-treated wounds at Day 7 expressed intense cytokeratin 10 staining near the wound junction in suprabasal epidermal layers, while staining in control tissues was less intense and restricted to apical epidermal layers distal to the wound junction. By Day 79, the staining was equal in both groups. However, PRP gel-treated wounds at Day 79 contained abundant, dense collagen bundles oriented parallel to each other and to the overlying epithelium, whereas control tissues contained fewer collagen fibers that were oriented randomly. Thus, treatment of wounds with PRP gel induced accelerated epithelial differentiation and produced tissue with organized, interlocking collagen bundles. This study reveals that this novel all-natural wound healant induced wound repair in injuries previously deemed untreatable.
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Publication Date: 2003-06-05 PubMed ID: 12782011DOI: 10.1016/s0014-4800(03)00017-0Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper looks at the deployment of platelet-rich plasma (PRP) gel in assisting the healing of wounds on horses’ lower limbs – wounds that are typically difficult to heal. Through controlled experimentation with PRP gel, the study shows that the treatment expedited epithelial differentiation and produced well-organized dermal collagen, resulting in enhanced wound healing.

Objective and Approach of the Research

  • The research primarily focuses on the potential of utilizing platelet-rich plasma (PRP) gel to significantly ameliorate the healing of non-healing equine lower limb wounds.
  • Platelets were obtained from horse blood and activated with thrombin, which led to the onset of growth factor release. This made a platelet gel composed of platelet-rich plasma (PRP).
  • For the experimental setup, full thickness cutaneous wounds of 2.5 cm² were formed below the knee and hock of a thoroughbred horse. These wounds were then treated with the developed PRP gel and some were left untreated for comparison.

Methodology and Observations

  • Biopsy samples were collected on the 7th, 36th, and 79th day post-wounding for comparing the expression of differentiation markers and the progression of wound repair.
  • This was performed to evaluate the hypothesis that PRP gel-treatment would lead to quicker epithelial differentiation and better organization of dermal collagen compared to the untreated wounds.
  • The researchers used staining methods to validate their hypothesis – cytokeratin 10 staining for examining the differentiation and trichrome staining for analyzing the collagen re-establishment.
  • On the 7th day, PRP gel-assisted wounds showed intense cytokeratin 10 staining near the wound junction in suprabasal epidermal layers, compared to a less intense staining in the untreated wounds.
  • On day 79, the staining was found to be homogeneous across both groups. However, the PRP-treated wounds showed a significant amount of well-organized, parallel-aligned collagen bundles, while the control group had lesser, randomly oriented collagen fibres.

Conclusion

  • The treatment of horse wounds with PRP gel facilitated an accelerated epithelial differentiation, and developed tissue complete with arranged, interlocking collagen bundles.
  • This research indicates that the novel all-natural wound treatment using PRP gel could be a significant solution in managing injuries previously considered non-treatable.

Cite This Article

APA
Carter CA, Jolly DG, Worden CE, Hendren DG, Kane CJ. (2003). Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing. Exp Mol Pathol, 74(3), 244-255. https://doi.org/10.1016/s0014-4800(03)00017-0

Publication

Experimental and molecular pathology
ISSN: 0014-4800
NlmUniqueID: 0370711
Country: Netherlands
Language: English
Volume: 74
Issue: 3
Pages: 244-255

Researcher Affiliations

Carter, Charleata A
  • Research Division, BeluMedX Equine Wound Healing, Little Rock, AR 72212, USA. charleatacarter@yahoo.com
Jolly, David G
    Worden, Charles E
      Hendren, Dennis G
        Kane, Cynthia J M

          MeSH Terms

          • Animals
          • Blood Platelets / drug effects
          • Blood Platelets / metabolism
          • Cell Differentiation
          • Collagen / metabolism
          • Enzyme-Linked Immunosorbent Assay
          • Gels / chemistry
          • Horse Diseases / drug therapy
          • Horse Diseases / pathology
          • Horse Diseases / physiopathology
          • Horses
          • Immunoenzyme Techniques
          • Keratin-10
          • Keratins / analysis
          • Keratins / metabolism
          • Male
          • Platelet-Derived Growth Factor / administration & dosage
          • Platelet-Derived Growth Factor / analysis
          • Platelet-Derived Growth Factor / metabolism
          • Regeneration
          • Skin / injuries
          • Skin / pathology
          • Thrombin / pharmacology
          • Transforming Growth Factor beta / analysis
          • Transforming Growth Factor beta / metabolism
          • Transforming Growth Factor beta1
          • Wound Healing / drug effects
          • Wound Healing / physiology

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