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Home / Equine Research Bank / Lipids / Positional distribution of fatty acids in triglycerides from...
Lipids1982; 17(6); 437-442; doi: 10.1007/BF02535223

Positional distribution of fatty acids in triglycerides from milk of several species of mammals.

Abstract: Milk triglycerides from the echidna, koala, Tammar wallaby, guinea pig, dog, cat, Weddell seal, horse, pig and cow were subjected to fatty acid and stereospecific analysis to determine the positional distribution of the fatty acids in the triglycerides. The samples presented a wide range of fatty acids, most of which varied in content among species. The compositions of the acids at the 3 positions also varied among species, reflecting the content of these acids in the triglycerides. However, there was a general similarity in fatty acid positional distribution patterns for all the species with the exception of the echidna. The echidna exhibited a completely different fatty acid positional distribution pattern. The saturated acids were preferentialy esterified at the sn-1-position whereas the unsaturated acids were selectively esterified at the sn-2-position. The triglyceride carbon number distribution of milk from the above species (with the exception of the Weddell seal) was determined by gas liquid chromatography and compared to that predicted by the 1-random-2-random-3-random fatty acid distribution hypothesis. Agreement was excellent between observed and predicted composition for echidna, koala, Tammar wallaby, guinea pig and pig milk, and agreement was reasonable for dog, cat, horse and cow milk. Results are discussed in relation to biochemical mechanisms.
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Publication Date: 1982-06-01 PubMed ID: 7109856DOI: 10.1007/BF02535223Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the variations in fatty acid positions within milk triglycerides across different mammal species including the echidna, koala, Tammar wallaby, guinea pig, dog, cat, Weddell seal, horse, pig, and cow. By conducting fatty acid analysis, the researchers uncovered varying compositions across species, with echidna demonstrating a unique pattern, eschewing the typical distribution seen in most mammals.

Objectives and Methodology

  • The objective of the study was to determine the differences in the positional distribution of fatty acids in the triglycerides from the milk of several mammal species.
  • Fatty acid and stereospecific analyses were conducted on milk triglycerides from each of the aforementioned mammal species. Additionally, gas-liquid chromatography was used to identify the carbon distribution of these triglycerides.

Main Findings

  • The study resulted in a broad spectrum of fatty acids, with variation of content among different species. This implies that the distribution of fatty acids within triglycerides is not universal across all mammals.
  • The study found that all species except the echidna demonstrated a general similarity in fatty acid positional distribution patterns.
  • The echidna presented a completely unique fatty acid distribution pattern. Saturated acids were predominantly esterified at the sn-1 position, while unsaturated acids were selectively esterified at the sn-2 position. This individual discrepancy reinforced the overall finding of varying triglyceride compositions across mammal species.

Validation Against the Random Fatty Acid Distribution Hypothesis

  • The researchers compared the results against the 1-random-2-random-3-random fatty acid distribution hypothesis. This is a theory that predicts a random distribution of fatty acids in the triglyceride positions.
  • Excellent agreement was found between observed and predicted distribution for echidna, koala, Tammar wallaby, guinea pig, and pig milk. Dog, cat, horse, and cow milk also demonstrated reasonable agreement. Despite some exceptions, this validation supports the hypothesis that fatty acid positions within triglycerides are generally random.

Implications and Conclusion

  • The study’s findings indicate that biochemical mechanisms managing fat absorption and metabolism could significantly vary across mammalian species.
  • A deeper understanding of these mechanisms could have implications for nutrition and health, particularly in mammalian young who depend on maternal milk for sustenance.
  • Further research is required to explore the causes of these variations and their potential implications.

Cite This Article

APA
Parodi PW. (1982). Positional distribution of fatty acids in triglycerides from milk of several species of mammals. Lipids, 17(6), 437-442. https://doi.org/10.1007/BF02535223

Publication

Lipids
ISSN: 0024-4201
NlmUniqueID: 0060450
Country: United States
Language: English
Volume: 17
Issue: 6
Pages: 437-442

Researcher Affiliations

Parodi, P W

    MeSH Terms

    • Animals
    • Cattle
    • Chromatography, Gas
    • Fatty Acids / analysis
    • Female
    • Horses
    • Marsupialia
    • Milk / analysis
    • Seals, Earless
    • Species Specificity
    • Swine
    • Triglycerides / analysis

    References

    This article includes 22 references
    1. J Lipid Res. 1965 Jan;6:10-5
      pubmed: 14280457
    2. Lipids. 1976 Jan;11(1):49-60
      pubmed: 1250066
    3. Biochim Biophys Acta. 1973 Jul 19;316(1):109-13
      pubmed: 4722463
    4. J Lipid Res. 1976 Nov;17(6):647-56
      pubmed: 11264
    5. Lipids. 1977 Dec;12(12):1005-11
      pubmed: 593067
    6. Biochem J. 1980 May 1;187(2):289-95
      pubmed: 7396849
    7. J Dairy Res. 1982 Feb;49(1):73-80
      pubmed: 7076946
    8. Can J Biochem. 1969 Aug;47(8):761-9
      pubmed: 5810867
    9. Biochim Biophys Acta. 1977 Nov 24;489(2):236-41
      pubmed: 922027
    10. Biochim Biophys Acta. 1969 Apr 29;176(3):445-52
      pubmed: 5800036
    11. Biochim Biophys Acta. 1970 Jun 9;210(1):46-56
      pubmed: 5456045
    12. Lipids. 1966 Nov;1(6):444-8
      pubmed: 17805654
    13. Lipids. 1976 Sep;11(9):680-4
      pubmed: 994758
    14. Lipids. 1974 Apr;9(4):229-34
      pubmed: 4833433
    15. Eur J Biochem. 1979 Feb 15;94(1):93-8
      pubmed: 436848
    16. Prog Lipid Res. 1979;17(4):347-95
      pubmed: 38463
    17. Lipids. 1974 Nov;9(11):905-12
      pubmed: 4215933
    18. Prog Lipid Res. 1980;19(1-2):23-106
      pubmed: 7232453
    19. J Lipid Res. 1966 Jan;7(1):62-4
      pubmed: 5900222
    20. Biochim Biophys Acta. 1966 Feb 1;116(1):67-72
      pubmed: 5942462
    21. Lipids. 1969 Nov;4(6):562-70
      pubmed: 5367940
    22. J Biol Chem. 1975 Aug 25;250(16):6315-9
      pubmed: 1158865

    Citations

    This article has been cited 15 times.
    1. Tadmor-Levi R, Argov-Argaman N. How Close Are We to the Production of Milk in Alternative Systems? The Fat Perspective. Foods 2025 Feb 26;14(5).
      doi: 10.3390/foods14050809pubmed: 40077512google scholar: lookup
    2. Zhou X, Zhao X, Parker L, Derkach P, Correa M, Benites V, Miller R, Athanasiadis D, Doherty B, Alnozaili G, Wittenberg J, Gates D, Destaillats F, Rakitsky W, Franklin S. Development and large-scale production of human milk fat analog by fermentation of microalgae. Front Nutr 2024;11:1341527.
      doi: 10.3389/fnut.2024.1341527pubmed: 38352706google scholar: lookup
    3. Tumino S, Bognanno M, Chessari G, Tolone M, Bordonaro S, Mangano F, Marletta D, Avondo M. Polymorphisms at Candidate Genes for Fat Content and Fatty Acids Composition: Effects on Sheep Milk Production and Fatty Acid Profile Using Two Dietary Supplementations. Animals (Basel) 2023 Aug 6;13(15).
      doi: 10.3390/ani13152533pubmed: 37570341google scholar: lookup
    4. Ren C, Jin J, Huppertz T, Zhang Y, Jin Q, Wang X. Evaluation of Fatty Acid Distributions and Triacylglycerol Species in Sow Milk and Commercial Piglet Formulas: A Comparative Study Based on Fat Sources and Lactation Stages. Animals (Basel) 2022 Dec 28;13(1).
      doi: 10.3390/ani13010124pubmed: 36611734google scholar: lookup
    5. Koziolek M, Carrière F, Porter CJH. Lipids in the Stomach - Implications for the Evaluation of Food Effects on Oral Drug Absorption. Pharm Res 2018 Feb 8;35(3):55.
      doi: 10.1007/s11095-017-2289-xpubmed: 29423732google scholar: lookup
    6. Parodi PW, Griffiths M. A comparison of the positional distribution of fatty acids in milk triglycerides of the extant monotremes platypus (Ornithorhynchus anatinus) and echidna (Tachyglossus aculeatus). Lipids 1983 Nov;18(11):845-7.
      doi: 10.1007/BF02534646pubmed: 27519991google scholar: lookup
    7. Zhang Z, Hou R, Lan J, Wang H, Kurokawa H, Takatsu Z, Kobayashi T, Koie H, Kamata H, Kanayama K, Watanabe T. Analysis of the breast milk of giant pandas (Ailuropoda melanoleuca) and the preparation of substitutes. J Vet Med Sci 2016 Jun 1;78(5):747-54.
      doi: 10.1292/jvms.15-0677pubmed: 26781707google scholar: lookup
    8. Kalo P, Kemppinen A, Ollilainen V, Kuksis A. Regiospecific determination of short-chain triacylglycerols in butterfat by normal-phase HPLC with on-line electrospray-tandem mass spectrometry. Lipids 2004 Sep;39(9):915-28.
      doi: 10.1007/s11745-004-1314-3pubmed: 15669768google scholar: lookup
    9. Kalo P, Kemppinen A, Kilpeläinen I. Determination of positional distribution of butyryl groups in milkfat triacylglycerols, triacylglycerol mixtures, and isolated positional isomers of triacylglycerols by gas chromatography and 1H nuclear magnetic resonance spectroscopy. Lipids 1996 Mar;31(3):331-6.
      doi: 10.1007/BF02529880pubmed: 8900463google scholar: lookup
    10. Jensen MM, Sørensen H, Høy CE. Influence of triacylglycerol structure and fatty acid profile of dietary fats on milk triacylglycerols in the rat. A two-generation study. Lipids 1996 Feb;31(2):187-92.
      doi: 10.1007/BF02522619pubmed: 8835407google scholar: lookup
    11. Innis SM, Dyer R, Quinlan PT, Diersen-Schade D. Dietary triacylglycerol structure and saturated fat alter plasma and tissue fatty acids in piglets. Lipids 1996 May;31(5):497-505.
      doi: 10.1007/BF02522643pubmed: 8727642google scholar: lookup
    12. Myher JJ, Kuksis A, Steiner G. Milk fat structure of a patient with type 1 hyperlipidemia. Lipids 1984 Sep;19(9):673-82.
      doi: 10.1007/BF02534527pubmed: 6503629google scholar: lookup
    13. Iverson SJ, Oftedal OT. Fatty acid composition of black bear (Ursus americanus) milk during and after the period of winter dormancy. Lipids 1992 Nov;27(11):940-3.
      doi: 10.1007/BF02535877pubmed: 1491615google scholar: lookup
    14. Iverson SJ, Sampugna J, Oftedal OT. Positional specificity of gastric hydrolysis of long-chain n-3 polyunsaturated fatty acids of seal milk triglycerides. Lipids 1992 Nov;27(11):870-8.
      doi: 10.1007/BF02535866pubmed: 1491605google scholar: lookup
    15. Puppione DL, Kuehlthau CM, Jandacek RJ, Costa DP. Positional analyses of triacylglycerol fatty acids in the milk fat of the antarctic fur seal (Arctocephalus gazella). Lipids 1992 Aug;27(8):637-9.
      doi: 10.1007/BF02536124pubmed: 1406076google scholar: lookup
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