Povidone-iodine lavage treatment of experimentally induced equine infectious arthritis.
Abstract: Both tarsocrural joints of 4 horses were inoculated with 1.5 X 10(5) colony-forming units of Staphylococcus aureus. On days 1, 3, and 6, each horse had one tarsocrural joint lavaged with a balanced electrolyte solution and had the contralateral tarsocrural joint lavaged with 0.1% povidone-iodine solution. All horses were orally administered trimethoprim (5 mg/kg)/sufadiazine (25 mg/kg) combination twice daily and phenylbutazone (2 g) once daily for the duration of the study (21 days). On days 0, 1, 3, 6, 9, 14, and 21, synovial fluid specimens were collected and analyzed for color, clarity, total protein concentration, WBC count and differential, and mucin clot-forming ability. Synovial fluid specimens collected on days 1, 3, 6, 9, 14, and 21 were bacteriologically cultured. On day 21, all horses were euthanatized, the tarsocrural joints were opened and examined, synovial membrane specimens were collected, bacteriologically cultured, and histologically evaluated, and articular cartilage specimens were histochemically evaluated. Repeated measures analysis of variance were used to evaluate differences between lavage solutions and among days for objective measurements. A paired t test was used to evaluate differences between solutions for the indices of synovial membrane inflammation and articular cartilage staining intensity with safranin-O-fast green. To be considered significant, the probability of a type-I error was less than 0.05. Significant differences were not found between joints lavaged with electrolyte solution vs povidone-iodine solution for synovial total protein concentration, WBC count, results of synovial fluid and membrane bacteriologic culture, synovial membrane inflammation, or articular cartilage glycosaminoglycan concentration.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1987-04-01 PubMed ID: 3592370
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- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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This research is about comparing the effectiveness of povidone-iodine solution with balanced electrolyte solution when used for lavage treatment of infectious arthritis caused by Staphylococcus aureus in horses.
Methodology
The study was carried out by inducing infectious arthritis in both tarsocrural joints of 4 horses by injecting them with 1.5 X 10(5) colony-forming units of Staphylococcus aureus.
- One tarsocrural joint of each horse was lavaged with balanced electrolyte solution, while the contralateral tarsocrural joint was lavaged with 0.1% povidone-iodine solution. This procedure was carried out on days 1, 3, and 6.
- As part of the treatment protocol, all horses were also orally administered trimethoprim (5 mg/kg)/sufadiazine (25 mg/kg) combination twice daily and phenylbutazone (2 g) once daily for the period of 21 days which was the study duration.
- Synovial fluid specimens were collected and examined on days 0, 1, 3, 6, 9, 14, and 21. These specimens were analyzed for color, clarity, total protein concentration, WBC count and differential, mucin clot-forming ability, and bacteriological culture on certain days.
- On the final day of the study (day 21), all horses were euthanatized and the tarsocrural joints were opened for examination. Synovial membrane specimens and articular cartilage specimens were also collected and evaluated bacteriologically, histologically, and histochemically.
Statistical Analysis
- The research used statistical analysis methods like repeated measures analysis of variance to evaluate differences between lavage solutions used and among days for objective measurements.
- A paired t test was applied to evaluate differences between solutions for the indices of synovial membrane inflammation and cartilage staining intensity.
- A result was considered statistically significant if the probability of a type-I error was less than 0.05.
Findings
The research found no significant differences between joints lavaged with the electrolyte solution or povidone-iodine solution when comparing synovial total protein concentration, WBC count, results of synovial fluid and membrane bacteriologic culture, synovial membrane inflammation, or articular cartilage glycosaminoglycan concentration. This indicates that both types of solution are comparably effective in treating infectious arthritis in the horses.
Cite This Article
APA
Bertone AL, McIlwraith CW, Jones RL, Norrdin RW, Radin MJ.
(1987).
Povidone-iodine lavage treatment of experimentally induced equine infectious arthritis.
Am J Vet Res, 48(4), 712-715.
Publication
Researcher Affiliations
MeSH Terms
- Analysis of Variance
- Animals
- Arthritis, Infectious / pathology
- Arthritis, Infectious / therapy
- Arthritis, Infectious / veterinary
- Horse Diseases / pathology
- Horse Diseases / therapy
- Horses
- Joints / pathology
- Povidone / analogs & derivatives
- Povidone-Iodine / therapeutic use
- Staphylococcal Infections / pathology
- Staphylococcal Infections / therapy
- Staphylococcal Infections / veterinary
- Synovial Fluid / analysis
- Synovial Fluid / cytology
- Synovial Fluid / microbiology
- Synovial Membrane / microbiology
- Synovial Membrane / pathology
- Therapeutic Irrigation / veterinary
Citations
This article has been cited 3 times.- Koziy RV, Yoshimura S, Dickinson R, Rybicka JM, Moshynskyy I, Ngeleka M, Bracamonte JL, Simko E. Use of standard diagnostic techniques to determine eradication of infection in experimental equine septic arthritis. Can J Vet Res 2019 Jan;83(1):24-33.
- Francoz D, Desrochers A, Latouche JS. Effect of repeated arthrocentesis and single joint lavage on cytologic evaluation of synovial fluid in 5 young calves. Can J Vet Res 2007 Apr;71(2):129-34.
- Francoz D, Desrochers A, Fecteau G, Desautels C, Latouche JS, Fortin M. Synovial fluid changes in induced infectious arthritis in calves. J Vet Intern Med 2005 May-Jun;19(3):336-43.
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