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Home / Equine Research Bank / Vet J / Practical considerations for clinical use of mesenchymal ste...
Veterinary journal (London, England : 1997)2018; 238; 49-57; doi: 10.1016/j.tvjl.2018.07.004

Practical considerations for clinical use of mesenchymal stem cells: From the laboratory to the horse.

Abstract: Since the clinical use of mesenchymal stem cells (MSCs) for treating musculoskeletal injuries is gaining popularity, practitioners should be aware of the factors that may affect MSCs from tissue harvesting for MSC isolation to cell delivery into the injury site. This review provides equine practitioners with up-to-date, practical knowledge for the treatment of equine patients using MSCs. A brief overview of laboratory procedures affecting MSCs is provided, but the main focus is on shipping conditions, routes of administration, injection methods, and which commonly used products can be combined with MSCs and which products should be avoided as they have deleterious effects on cells. There are still several knowledge gaps regarding MSC-based therapies in horses. Therefore, it is important to properly manage the factors which are currently known to affect MSCs, to further strengthen the evidence basis of this treatment.
Copyright © 2018 Elsevier Ltd. All rights reserved.
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Publication Date: 2018-07-17 PubMed ID: 30103915DOI: 10.1016/j.tvjl.2018.07.004Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the use of mesenchymal stem cells (MSCs) in treating musculoskeletal injuries in horses and provides practical knowledge on handling these cells from tissue harvesting to delivering into the injury site.

Laboratory Procedures and MSCs

  • The article starts by providing a brief overview of the laboratory procedures involving MSCs. In this context, the researchers elucidate how tissue harvesting for MSC isolation is done and what factors may affect the same.
  • This section acts as a roadmap for researchers or practitioners who work in labs and assists them in understanding the nuances of working with MSCs. Specific procedures are not detailed in the abstract, however, it can be assumed that these might include methods of cell culture, storage, and proliferation.

Shipping Conditions

  • Handling MSCs extends beyond the laboratory and includes the way they are transported or shipped. The research reveals that there are specific shipping conditions that ensure the cells remain viable and potent for treating the injuries.
  • Although not detailed in the abstract, shipping conditions might pertain to aspects such as temperature control, maintaining appropriate cell environment, and proper packaging to avoid contamination.

Routes of Administration and Injection Methods

  • The study also focuses on how MSCs are administered to equine patients. It mentions that there are certain routes and injection methods that are appropriate for the administration of these cells.
  • Specific methods or routes aren’t detailed in this summary, but they would generally include options like intravenous injection, localized injection at the injury site, etc.

Combination Products

  • The research also mentions about the use of combination products with MSCs. Some products are identified to have potential benefits when combined with MSCs, whilst others are advised to be avoided as they may have harmful effects.
  • These findings may prove important for practitioners and could potentially open up new treatment avenues for researchers studying MSC-based therapies.

Current Knowledge Gaps

  • Despite the progress made in understanding and utilizing MSCs for equine injury treatment, the research acknowledges that there still exist several knowledge gaps.
  • The abstract emphasizes minimizing the impact of known factors that negatively impact MSCs while noting the ongoing process of research to fully comprehend and develop MSC-based therapies.

Cite This Article

APA
Barrachina L, Romero A, Zaragoza P, Rodellar C, Vázquez FJ. (2018). Practical considerations for clinical use of mesenchymal stem cells: From the laboratory to the horse. Vet J, 238, 49-57. https://doi.org/10.1016/j.tvjl.2018.07.004

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 238
Pages: 49-57
PII: S1090-0233(18)30379-4

Researcher Affiliations

Barrachina, L
  • Laboratorio de Genética Bioquímica LAGENBIO, Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, 50013 Zaragoza, Spain.
Romero, A
  • Laboratorio de Genética Bioquímica LAGENBIO, Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, 50013 Zaragoza, Spain.
Zaragoza, P
  • Laboratorio de Genética Bioquímica LAGENBIO, Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, Zaragoza, Spain; Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-Centro de Investigación y Tecnología de Aragón (CITA), Zaragoza, Spain.
Rodellar, C
  • Laboratorio de Genética Bioquímica LAGENBIO, Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, Zaragoza, Spain; Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-Centro de Investigación y Tecnología de Aragón (CITA), Zaragoza, Spain.
Vázquez, F J
  • Laboratorio de Genética Bioquímica LAGENBIO, Instituto de Investigación Sanitaria de Aragón (IIS), Universidad de Zaragoza, Zaragoza, Spain; Servicio de Cirugía y Medicina Equina, Hospital Veterinario, Universidad de Zaragoza, 50013 Zaragoza, Spain. Electronic address: pvazquez@unizar.es.

MeSH Terms

  • Animals
  • Cell Culture Techniques
  • Horse Diseases / therapy
  • Horses
  • Mesenchymal Stem Cell Transplantation / methods
  • Mesenchymal Stem Cell Transplantation / veterinary
  • Mesenchymal Stem Cells / cytology
  • Musculoskeletal Diseases / therapy
  • Musculoskeletal Diseases / veterinary
  • Regenerative Medicine

Citations

This article has been cited 14 times.
  1. Phyo H, Aburza A, Mellanby K, Esteves CL. Characterization of canine adipose- and endometrium-derived Mesenchymal Stem/Stromal Cells and response to lipopolysaccharide. Front Vet Sci 2023;10:1180760.
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  3. Cahalan SD, Boehm I, Jones RA, Piercy RJ. Recognising the potential of large animals for modelling neuromuscular junction physiology and disease. J Anat 2022 Nov;241(5):1120-1132.
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    doi: 10.3390/life15101558pubmed: 41157231google scholar: lookup
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    doi: 10.3390/ijms25063426pubmed: 38542399google scholar: lookup
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