Practical experience with artificial insemination (AI) using fresh chilled and frozen semen in mares.
Abstract: The objective of this study was to compare the efficiency of artificial insemination (AI) carried out with frozen and fresh, diluted and chilled semen under field conditions. One hundred and twenty-nine mares of different breeds were included in the study. Eighty-one out of the 107 mares inseminated with fresh, chilled semen got pregnant. Seven pregnant mares aborted and 74 foals were born. Out of the 22 mares inseminated with frozen semen, 17 mares got pregnant. Two mares out of the 17 pregnant mares aborted and finally 15 healthy foals were born. No difference was found between the two groups in the ratio of the foals born (P > 0.05). The comparison of medians for the number of insemination cycles did not show significant differences. However, a significant difference (Kruskal-Wallis test, P = 0.014) was found in the number of the inseminations per conception in favour of frozen semen (2.5 vs. 1.8 with fresh chilled and frozen semen, respectively). The Cox regression revealed that the type of semen has a significant impact (P < 0.001) on the service period (duration of the insemination period): the use of frozen semen prolonged the insemination period. This could be due to management issues, since re-insemination with frozen semen took place after only one/a few missed oestrous cycles not used for AI.
Publication Date: 2020-05-08 PubMed ID: 32384059DOI: 10.1556/004.2020.00007Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Animal Science
- Artificial Insemination
- Assisted Reproductive Techniques
- Cryopreservation
- Equid Semen
- Equine Diseases
- Equine Health
- Equine Science
- Equine Studies
- Field Study
- Freezing Technique
- Mares
- Pregnancy
- Pregnant Mares' Serum Gonadotropin
- Reproduction
- Semen Preservation
- Veterinary Care
- Veterinary Medicine
- Veterinary Research
Summary
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This research article studies the efficiency of artificial insemination (AI) in horses under field conditions, comparing the results of using both fresh chilled and frozen semen. The findings suggest that while both methods lead to successful pregnancies and birth of foals, the use of frozen semen requires more insemination attempts and extends the insemination period.
Objective and Design of the Study
- The main aim of the study was to evaluate the effectiveness of artificial insemination (AI) using both frozen and fresh, diluted and chilled semen under practical conditions.
- One hundred and twenty-nine mares of varying breeds were included in the analysis.
- The study design was a comparative analysis of the two AI methods in terms of the resulting pregnancies, abortions, foal births and insemination cycles.
Comparison of the Two Methods
- Of the 107 mares inseminated with fresh, chilled semen, 81 became pregnant. Following seven abortions, 74 foals were born successfully.
- Compared to this, 17 out of 22 mares inseminated with frozen semen became pregnant. After two abortions, 15 healthy foals were born.
- The ratio of foals born did not show a significant difference between the two groups. This suggests that both methods were equally successful in leading to birth of foals.
- Further, the number of insemination cycles also did not showcase any significant difference between fresh chilled and frozen semen methods.
Difference in the Number of Inseminations and Duration of Insemination Period
- A significant difference was observed in the number of inseminations per conception, favouring frozen semen (2.5 vs. 1.8 with fresh chilled and frozen semen, respectively).
- This implies that more attempts were required to achieve conception when using frozen semen.
- Cox regression analysis further revealed that the type of semen has a significant impact on the duration of the insemination period. Using frozen semen was associated with a longer insemination period.
- This could potentially be due to management issues, as re-insemination with frozen semen happened after only one or a few missed oestrous cycles that were not used for artificial insemination.
Cite This Article
APA
Gáspárdy A, Renkó E, Somoskői B, Bába A, Cseh S.
(2020).
Practical experience with artificial insemination (AI) using fresh chilled and frozen semen in mares.
Acta Vet Hung, 68(1), 85-90.
https://doi.org/10.1556/004.2020.00007 Publication
Researcher Affiliations
- 1Department of Animal Breeding, Nutrition and Laboratory Animal Science, University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary.
- 2Department and Clinic of Reproduction, University of Veterinary Medicine, Budapest, Hungary.
- 2Department and Clinic of Reproduction, University of Veterinary Medicine, Budapest, Hungary.
- 3Polequi Bt., Budapest, Hungary.
- 2Department and Clinic of Reproduction, University of Veterinary Medicine, Budapest, Hungary.
MeSH Terms
- Animals
- Cryopreservation / veterinary
- Female
- Horses / physiology
- Hungary
- Insemination, Artificial / statistics & numerical data
- Insemination, Artificial / veterinary
- Semen Preservation / methods
- Semen Preservation / veterinary
Citations
This article has been cited 3 times.- Mahmood K, Ali Channa A, Ghafoor A, Riaz A. Factors affecting the efficiency of equine embryo transfer (EET) in polo mares under subtropical conditions of Pakistan. PLoS One 2024;19(2):e0298066.
- Vozaf J, Svoradová A, Baláži A, Vašíček J, Olexiková L, Dujíčková L, Makarevich AV, Jurčík R, Ďúranová H, Chrenek P. The Cryopreserved Sperm Traits of Various Ram Breeds: Towards Biodiversity Conservation. Animals (Basel) 2022 May 20;12(10).
- Ramirez-Perez H, Guerrero-Netro HM, Torres-Rodríguez P, Díaz-Durán M, Boeta-Acosta AM, Diaw M. A combination of taurine and caffeine maintains sperm quality in equine semen during chilled storage. J Adv Vet Anim Res 2021 Dec;8(4):635-641.
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