Pregnancy-associated endometrial expression of antileukoproteinase gene is correlated with epitheliochorial placentation.
Abstract: Uterine expression of the mRNA encoding antileukoproteinase (ALP) is highest in pig uterus during mid- to late pregnancy, suggesting a stage of pregnancy-dependent role for this elastase/cathepsin G protease inhibitor in feto-maternal interactions. To examine a potential relationship between uterine synthesis of ALP and the type of placentation in mammalian species, the expression of ALP mRNA and/or protein in pregnant mares, cows, rats, and mice was evaluated. Genomic DNA and mRNA hybridization analyses were performed using a porcine ALP cDNA as probe. The concentration of ALP protein in reproductive tissues was determined by RIA using a polyclonal antibody raised against a synthetic peptide (ALP 16P) corresponding to amino acid residues 21-36 of the porcine ALP protein. A single ALP mRNA transcript of approximately 0.8 kb in length was detected in equine and bovine uterine tissues. The relative abundance of ALP mRNA in equine endometrium increased between days 125-170 (mid-pregnancy), and then decreased by day 215 of pregnancy. Similarly, the steady state levels of ALP mRNA in bovine endometrium and myometrium were higher during mid- to late than during early pregnancy. The levels of ALP mRNA in bovine fetal cotyledon were low and did not change significantly with stage of pregnancy. No hybridization was detected to pregnant rat endometrial tissues, although high stringency Southern blot analysis of porcine, bovine, and rat genomic DNAs using porcine ALP cDNA as probe predicted a high degree of nucleotide sequence homology in their respective ALP genes.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1994-08-01 PubMed ID: 7980943DOI: 10.1002/mrd.1080380402Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research revolves around highlighting the role of the antileukoproteinase gene (ALP) in pregnancy, specifically in the feto-maternal interactions. It suggests that the ALP gene expression in the uterus during mid to late pregnancy can have a significant role in this interaction.
Study Focus
The paper primarily focusses on:
- Delineating the relationship between uterine synthesis of ALP and the type of placentation in mammals.
- Evaluating the expression of ALP mRNA (messenger RNA) and/or protein in pregnant mares, cows, rats, and mice.
Methods
The methods used to carry out this research concluded:
- Genomic DNA and mRNA hybridization analysis were performed using a pig ALP cDNA (complementary DNA) as a probe.
- The concentration of ALP protein in reproductive tissues were determined with a polyclonal antibody against a synthetic peptide.
Findings
Findings of the study includes:
- Detection of a single ALP mRNA transcript in equine and bovine uterine tissues.
- An increase in the relative abundance of ALP mRNA in equine endometrium between days 125-170 (mid-pregnancy), followed by a decrease by day 215 of pregnancy.
- Higher steady-state levels of ALP mRNA in bovine endometrium and myometrium during mid to late rather than early pregnancy.
- Low levels of ALP mRNA in bovine fetal cotyledon throughout pregnancy.
Conclusion
The absence of pregnant rat endometrial tissues despite the high stringency Southern blot analysis suggests that the ALP gene may play a significant role in mammalian pregnancy, particularly in the feto-maternal interactions during mid to late pregnancy stages. Further studies would cement the hypothesis and unravel deeper insights into the role of ALP in pregnancy.
Cite This Article
APA
Badinga L, Michel FJ, Fields MJ, Sharp DC, Simmen RC.
(1994).
Pregnancy-associated endometrial expression of antileukoproteinase gene is correlated with epitheliochorial placentation.
Mol Reprod Dev, 38(4), 357-363.
https://doi.org/10.1002/mrd.1080380402 Publication
Researcher Affiliations
- Animal Science Department, University of Florida, Gainesville 32611-0910.
MeSH Terms
- Animals
- Cattle / metabolism
- Enzyme Induction
- Female
- Gestational Age
- Horses / metabolism
- Mammals / anatomy & histology
- Mammals / metabolism
- Mice / metabolism
- Placentation
- Pregnancy
- Pregnancy Proteins / biosynthesis
- Pregnancy Proteins / genetics
- Pregnancy Proteins / physiology
- Pregnancy, Animal / metabolism
- Proteinase Inhibitory Proteins, Secretory
- Proteins
- Rats / metabolism
- Serine Proteinase Inhibitors / biosynthesis
- Serine Proteinase Inhibitors / genetics
- Serine Proteinase Inhibitors / physiology
- Species Specificity
- Swine / metabolism
Citations
This article has been cited 2 times.- Lee S, Yoo I, Cheon Y, Ka H. Spatiotemporal expression and regulation of peptidase inhibitor 3 and secretory leukocyte protease inhibitor at the maternal-fetal interface in pigs.. Anim Biosci 2023 Jul;36(7):1034-1043.
- Pfundt R, van Ruissen F, van Vlijmen-Willems IM, Alkemade HA, Zeeuwen PL, Jap PH, Dijkman H, Fransen J, Croes H, van Erp PE, Schalkwijk J. Constitutive and inducible expression of SKALP/elafin provides anti-elastase defense in human epithelia.. J Clin Invest 1996 Sep 15;98(6):1389-99.
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