Pregnancy outcome in mares following insemination deep in the uterine horn with low numbers of sperm selected by glass wool/Sephadex filtration, Percoll separation or absolute number.
Abstract: Mares were inseminated deep in the uterine horn with 25 million sperm selected by glass wool/Sephadex (GWS) filtration, Percoll separation (PS) or absolute number (AN). Deep-horn insemination using a low-volume, smooth tipped, flexible pipette/catheter delivery system allowed more efficient use of stallion sperm and reduced post-breeding uterine reaction in mares. Mares were pregnant in 15/30, 13/30 and 10/30 cycles for GWS, PS and AN selection methods, respectively. Sperm selection method did not effect pregnancy outcome (P=0.422). However, sperm selected for deep-horn insemination by filtration through a glass wool/Sephadex column tended to improve fertility over simply using an absolute number of sperm (P=0.105).
Publication Date: 2003-07-11 PubMed ID: 12853183DOI: 10.1016/s0378-4320(03)00086-1Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research explored the effectiveness of different sperm selection methods for deep uterine horn insemination in mares. It was found that using a glass wool/Sephadex filtration method tends to improve fertility more than just using an absolute number of sperm, although the method of selection didn’t significantly impact the pregnancy outcome.
Objective of the study
- The study aimed at determining the efficacy of three different sperm selection methods – glass wool/Sephadex (GWS) filtration, Percoll separation (PS), and absolute number (AN) – for deep uterine horn insemination in mares. The researchers examined the impact of these methods on both the efficient usage of stallion sperm and the occurrence of post-breeding uterine reactions.
Research Methodology
- Using a low-volume, smooth-tipped, flexible pipette/catheter delivery system, mares were inseminated deep in the uterine horn with 25 million sperm.
- The conception rates using the three different sperm selection methods were then observed and recorded.
Findings and Conclusions
- The study concluded that the method of sperm selection did not significantly affect the chances of pregnancy. This conclusion was reached after observing the conception rates in the mares inseminated using GWS, PS, and AN selection methods.
- The chances of pregnancy were found to be 15 out of 30 for mares inseminated using GWS, 13 out of 30 for mares inseminated using PS, and 10 out of 30 for mares inseminated using AN methods.
- However, it was noticed that using the GWS filtration method for sperm selection in deep-horn insemination tended to improve fertility rates more than just using an absolute number of sperm, even though it did not significantly alter the overall pregnancy outcome.
Significance of the study
- The observed slight improvement in fertility rates when using the GWS filtration method for sperm selection suggests this method could potentially be used to increase fertility in mares, especially if more research confirms these preliminary findings.
Cite This Article
APA
Nie GJ, Johnson KE, Wenzel JG.
(2003).
Pregnancy outcome in mares following insemination deep in the uterine horn with low numbers of sperm selected by glass wool/Sephadex filtration, Percoll separation or absolute number.
Anim Reprod Sci, 79(1-2), 103-109.
https://doi.org/10.1016/s0378-4320(03)00086-1 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, AL 36849-5522, USA. niegary@earthlink.net
MeSH Terms
- Animals
- Breeding
- Cell Separation / methods
- Centrifugation, Density Gradient
- Chromatography, Gel
- Dextrans
- Female
- Glass
- Horses
- Insemination, Artificial / methods
- Insemination, Artificial / veterinary
- Male
- Povidone
- Pregnancy
- Pregnancy Outcome / veterinary
- Silicon Dioxide
- Sperm Count
- Spermatozoa / cytology
Citations
This article has been cited 1 times.- Orsolini MF, Meyers SA, Dini P. An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section II.. Animals (Basel) 2021 Nov 20;11(11).
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