Preliminary characterization of equine interferons and their antiviral activities on bovine, ovine, and human cells.
Abstract: Equine dermal cells induced with poly I:C + DEAE-dextran produced low levels of interferon tentatively classified as equine interferon beta (EqIFN-beta). In contrast, dermal cells initially primed with EqIFN-beta and then superinduced with poly I:C + DEAE-dextran in the presence of cycloheximide and actinomycin D produced greater than 100-fold EqIFN-beta. Equine blood mononuclear cells induced with Newcastle disease virus and phytohemagglutinin produced high levels of interferons tentatively classified as equine interferon alpha (EqIFN-alpha) and equine interferon gamma (EqIFN-gamma), respectively. Both EqIFN-beta and EqIFN-gamma exhibited equivalent antiviral activity on equine, bovine, and ovine cells while EqIFN-alpha had greater activity on bovine and ovine cells than on equine cells. Furthermore, EqIFN-alpha had high antiviral activity on human cells. No detectable levels of antiviral activity of equine interferons was observed on feline and mouse cells.
Publication Date: 1982-01-01 PubMed ID: 6182253DOI: 10.1089/jir.1982.2.363Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research explores the production and anti-viral effectiveness of equine (horse) interferons on cells from various species including bovines (cows), ovines (sheep), and humans.
Interferon Production in Equine Cells
- Evolving from the study, it was noted that when induced with a combination of poly I:C + DEAE-dextran, equine dermal cells produced a low level of interferon initially classified as equine interferon beta (EqIFN-beta).
- Fascinatingly, when these dermal cells were first primed with EqIFN-beta and then superinduced – or triggered at an augmented rate – with the combo of poly I:C + DEAE-dextran, in the presence of cycloheximide and actinomycin D, the same cells produced over 100 times more EqIFN-beta.
Interferon Production in Equine Blood Mononuclear Cells
- Moreover, the study also examined equine blood mononuclear cells, finding that they produced high levels of interferons when induced with Newcastle disease virus and phytohemagglutinin. These interferons were tentatively classified as equine interferon alpha (EqIFN-alpha) and equine interferon gamma (EqIFN-gamma) respectively.
Interferon Anti-viral Activities on Different Species
- The research also investigated the antiviral activities of these equine interferons on cells of different species, including bovine, ovine, and human cells.
- It was found that both EqIFN-beta and EqIFN-gamma demonstrated equivalent antiviral activities on equine, bovine, and ovine cells.
- However, EqIFN-alpha indicated higher antiviral activity on bovine and ovine cells compared to equine cells, with particularly high activity on human cells.
- The study also highlighted that no detectable levels of antiviral activities of equine interferons were observed on cells from felines (cats) and mice.
Conclusion
- In summary, the research advances our understanding of equine interferons, the conditions which maximize their production, and their varying effectiveness as antiviral agents across different cell types from different species. This could potentially hold implications for the development of cross-species antiviral treatments in future.
Cite This Article
APA
Yilma T, McGuire TC, Perryman LE.
(1982).
Preliminary characterization of equine interferons and their antiviral activities on bovine, ovine, and human cells.
J Interferon Res, 2(3), 363-370.
https://doi.org/10.1089/jir.1982.2.363 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antiviral Agents
- Cattle
- Cells, Cultured
- Horses / immunology
- Humans
- Interferon Type I / biosynthesis
- Interferon-gamma / biosynthesis
- Interferons / isolation & purification
- Interferons / pharmacology
- Sheep
- Skin / immunology
- Species Specificity
Grant Funding
- HD08886 / NICHD NIH HHS
- RR05465-19 / NCRR NIH HHS
Citations
This article has been cited 4 times.- Detournay O, Morrison DA, Wagner B, Zarnegar B, Wattrang E. Genomic analysis and mRNA expression of equine type I interferon genes.. J Interferon Cytokine Res 2013 Dec;33(12):746-59.
- Papin JF, Verardi PH, Jones LA, Monge-Navarro F, Brault AC, Holbrook MR, Worthy MN, Freiberg AN, Yilma TD. Recombinant Rift Valley fever vaccines induce protective levels of antibody in baboons and resistance to lethal challenge in mice.. Proc Natl Acad Sci U S A 2011 Sep 6;108(36):14926-31.
- Jensen-Waern M, Persson SG, Nordengrahn A, Mérza M, Fossum C. Temporary suppression of cell-mediated immunity in standardbred horses with decreased athletic capacity.. Acta Vet Scand 1998;39(1):25-33.
- Bridges CG, Edington N. Innate immunity during Equid herpesvirus 1 (EHV-1) infection.. Clin Exp Immunol 1986 Jul;65(1):172-81.
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