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Pathogens (Basel, Switzerland)2023; 12(8); 1043; doi: 10.3390/pathogens12081043

Presence of Equine and Bovine Coronaviruses, Endoparasites, and Bacteria in Fecal Samples of Horses with Colic.

Abstract: Acute abdominal pain (colic) is one of the major equine health threats worldwide and often necessitates intensive veterinary medical care and surgical intervention. Equine coronavirus (ECoV) infections can cause colic in horses but are rarely considered as a differential diagnosis. To determine the frequency of otherwise undetected ECoV infections in horses with acute colic, fresh fecal samples of 105 horses with acute colic and 36 healthy control horses were screened for viruses belonging to the species by RT-PCR as well as for gastrointestinal helminths and bacteria commonly associated with colic. Horses with colic excreted significantly fewer strongyle eggs than horses without colic. The prevalence of anaerobic, spore-forming, gram-positive bacteria ( and ) was significantly higher in the feces of horses with colic. Six horses with colic (5.7%) and one horse from the control group (2.8%) tested positive for Betacoronaviruses. Coronavirus-positive samples were sequenced to classify the virus by molecular phylogeny (N gene). Interestingly, in three out of six coronavirus-positive horses with colic, sequences closely related to bovine coronaviruses (BCoV) were found. The pathogenic potential of BCoV in horses remains unclear and warrants further investigation.
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Publication Date: 2023-08-15 PubMed ID: 37624003PubMed Central: PMC10458731DOI: 10.3390/pathogens12081043Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the prevalence of Equine coronavirus (ECoV), along with endoparasites and bacteria, in horses suffering from colic, a condition characterised by severe abdominal pain. A comparative analysis was also carried out with healthy control horses. The findings revealed a significantly higher presence of certain types of bacteria and occasional detection of coronaviruses related to bovine coronaviruses in horses with colic.

Study Methodology and Sample Population

  • The study involved the examination of fresh fecal samples from 105 horses having acute colic and 36 healthy control horses.
  • The research sought to determine the degree of undiagnosed ECoV infections in horses that presented with acute colic, which is a significant health issue in horses on a global scale.
  • Specifically, the researchers screened for the existence of viruses of the Betacoronavirus species using RT-PCR.
  • The team also examined the samples for gastrointestinal helminths and bacteria that are often linked to colic.

Key Findings

  • Horses with colic were found to excrete significantly fewer strongyle eggs than horses without colic.
  • Importantly, the feces of horses with colic had a significantly higher occurrence of anaerobic, spore-forming, gram-positive bacteria, specifically Clostridium and Paeniclostridium spp.
  • Six of the horses with colic (representing 5.7% of the cohort) and one horse from the control group (comprising 2.8% of the control group) tested positive for Betacoronaviruses.

Observations on Coronavirus Detection

  • The coronavirus-positive samples underwent sequencing to classify the virus by molecular phylogeny, specifically through the N gene.
  • Interestingly, in three of the six coronavirus-positive horses with colic, bovine coronaviruses (BCoV)-related sequences were detected.
  • The discovery of BCoV-related sequences in horses raises questions about the virus’s potential pathogenicity in equines and necessitates further research.

Cite This Article

APA
Stummer M, Frisch V, Glitz F, Hinney B, Spergser J, Krücken J, Diekmann I, Dimmel K, Riedel C, Cavalleri JV, Rümenapf T, Joachim A, Lyrakis M, Auer A. (2023). Presence of Equine and Bovine Coronaviruses, Endoparasites, and Bacteria in Fecal Samples of Horses with Colic. Pathogens, 12(8), 1043. https://doi.org/10.3390/pathogens12081043

Publication

Pathogens (Basel, Switzerland)
ISSN: 2076-0817
NlmUniqueID: 101596317
Country: Switzerland
Language: English
Volume: 12
Issue: 8
PII: 1043

Researcher Affiliations

Stummer, Moritz
  • Institute of Virology, University of Veterinary Medicine, 1210 Vienna, Austria.
Frisch, Vicky
  • Clinical Unit of Equine Internal Medicine, University of Veterinary Medicine, 1210 Vienna, Austria.
Glitz, Frauke
  • Animal Clinic Würflach, 2732 Würflach, Austria.
Hinney, Barbara
  • Institute of Parasitology, University of Veterinary Medicine, 1210 Vienna, Austria.
Spergser, Joachim
  • Institute of Microbiology, University of Veterinary Medicine, 1210 Vienna, Austria.
Krücken, Jürgen
  • Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.
Diekmann, Irina
  • Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany.
Dimmel, Katharina
  • Institute of Virology, University of Veterinary Medicine, 1210 Vienna, Austria.
Riedel, Christiane
  • CIRI-Centre International de Recherche en Infectiologie, Univ Lyon, Université Claude Bernard Lyon 1, Inserm, U1111, CNRS, UMR5308, ENS Lyon, 46 allée d'Italie, 69364 Lyon, France.
Cavalleri, Jessika-Maximiliane V
  • Clinical Unit of Equine Internal Medicine, University of Veterinary Medicine, 1210 Vienna, Austria.
Rümenapf, Till
  • Institute of Virology, University of Veterinary Medicine, 1210 Vienna, Austria.
Joachim, Anja
  • Institute of Parasitology, University of Veterinary Medicine, 1210 Vienna, Austria.
Lyrakis, Manolis
  • Platform for Bioinformatics and Biostatistics, Department of Biomedical Sciences, University of Veterinary Medicine, 1210 Vienna, Austria.
Auer, Angelika
  • Institute of Virology, University of Veterinary Medicine, 1210 Vienna, Austria.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 3 times.
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  2. Aziz KJ, Barwary LTOA, Issa NA, Abdulwahid MJ. Prevalence of Theileria Equi and Babesia Caballi and Associated Hemato-Biochemical Changes in Racing Horses in Erbil Province, Iraq. Acta Parasitol 2025 Jan 24;70(1):38.
    doi: 10.1007/s11686-024-00987-9pubmed: 39853481google scholar: lookup
  3. Haywood LMB, Sheahan BJ. A Review of Epithelial Ion Transporters and Their Roles in Equine Infectious Colitis. Vet Sci 2024 Oct 7;11(10).
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