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The Journal of veterinary medical science2011; 73(12); 1663-1667; doi: 10.1292/jvms.11-0140

Prevalence of equine herpesvirus type 1 strains of neuropathogenic genotype in a major breeding area of Japan.

Abstract: A single non-synonymous nucleotide substitution of guanine (G) for adenine (A) at position 2254 in the viral DNA polymerase gene (encoded by open reading frame [ORF] 30) of equine herpesvirus type 1 (EHV-1) has been significantly associated with neuropathogenic potential in strains of this virus. To estimate the prevalence of EHV-1 strains with the neuropathogenic genotype (ORF30 G(2254)) in the Hidaka district--a major horse breeding area in Japan--we analyzed the ORF30 genomic region in cases of EHV-1 infection in this area during the years 2001-2010. Of the 113 cases analyzed, 3 (2.7%) were induced by ORF30 G(2254) strains. This prevalence is lower than those observed in the U.S.A. (10.8-19.4%), Argentina (7.4%), France (24%), and Germany (10.6%).
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Publication Date: 2011-08-09 PubMed ID: 21828961DOI: 10.1292/jvms.11-0140Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the prevalence of neuropathogenic equine herpesvirus type 1 (EHV-1) strains in the Hidaka region of Japan. It shows that these strains were involved in 2.7% of EHV-1 infections between 2001 and 2010, which is a lower prevalence than reported in other countries.

Objective of the Research

  • The primary objective of this research was to determine the prevalence of strains of equine herpesvirus type 1 (EHV-1) with a neuropathogenic genotype, specifically possessing a single non-synonymous nucleotide substitution of guanine (G) for adenine (A) at position 2254 within the viral DNA polymerase gene (encoded by open reading frame [ORF] 30). This substitution has been found to greatly enhance the neuropathogenic potential of EHV-1 strains. The place of study was the Hidaka district in Japan, a significant area for horse breeding.

Methodology of the Study

  • Researchers studied cases of EHV-1 infection in the Hidaka region over a decade, from 2001 to 2010. They focused on analyzing the ORF30 genomic region where the aforementioned substitution occurs.

Main Findings

  • From 113 cases of EHV-1 infection analyzed, the researchers found only 3 cases (or 2.7% of the total) to be induced by ORF30 G(2254) strains (neuropathogenic genotype). This suggests that the prevalence of neuropathogenic genotypes in the Hidaka region is relatively low.
  • The prevalence rate in Hidaka is markedly lower when compared to observed rates in other countries, including the USA (10.8-19.4%), Argentina (7.4%), France (24%), and Germany (10.6%). This comparison adds context to the findings and highlights the relative rarity of the neuropathogenic genotype in this particular region of Japan.

Significance of the Research

  • The findings provide important insights into the prevalence and geographical distribution of EHV-1 strains with the neuropathogenic genotype. Understanding this prevalence can guide appropriate preventative and treatment measures in areas of high horse breeding activity, such as Hidaka.
  • By determining that neuropathogenic strains have a lower prevalence than previously expected, the study may contribute to updated infectious disease control strategies and health policies in the equine industry, catering to specific regional requirements.

Cite This Article

APA
Tsujimura K, Oyama T, Katayama Y, Muranaka M, Bannai H, Nemoto M, Yamanaka T, Kondo T, Kato M, Matsumura T. (2011). Prevalence of equine herpesvirus type 1 strains of neuropathogenic genotype in a major breeding area of Japan. J Vet Med Sci, 73(12), 1663-1667. https://doi.org/10.1292/jvms.11-0140

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 73
Issue: 12
Pages: 1663-1667

Researcher Affiliations

Tsujimura, Koji
  • Epizootic Research Center, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329–0412, Japan. koji_tsujimura@jra.go.jp
Oyama, Takeshi
    Katayama, Yoshinari
      Muranaka, Masanori
        Bannai, Hiroshi
          Nemoto, Manabu
            Yamanaka, Takashi
              Kondo, Takashi
                Kato, Masakatsu
                  Matsumura, Tomio

                    MeSH Terms

                    • Abortion, Veterinary
                    • Animals
                    • Breeding
                    • Female
                    • Herpesviridae Infections / epidemiology
                    • Herpesviridae Infections / veterinary
                    • Herpesviridae Infections / virology
                    • Herpesvirus 1, Equid / genetics
                    • Herpesvirus 1, Equid / isolation & purification
                    • Horse Diseases / epidemiology
                    • Horse Diseases / virology
                    • Horses
                    • Japan / epidemiology
                    • Pregnancy
                    • Pregnancy Complications, Infectious
                    • Time Factors

                    Citations

                    This article has been cited 17 times.
                    1. Carvelli A, Nielsen SS, Paillot R, Broglia A, Kohnle L. Clinical impact, diagnosis and control of Equine Herpesvirus-1 infection in Europe. EFSA J 2022 Apr;20(4):e07230.
                      doi: 10.2903/j.efsa.2022.7230pubmed: 35414834google scholar: lookup
                    2. Tong P, Duan R, Palidan N, Deng H, Duan L, Ren M, Song X, Jia C, Tian S, Yang E, Kuang L, Xie J. Outbreak of neuropathogenic equid herpesvirus 1 causing abortions in Yili horses of Zhaosu, North Xinjiang, China. BMC Vet Res 2022 Mar 1;18(1):83.
                      doi: 10.1186/s12917-022-03171-1pubmed: 35232435google scholar: lookup
                    3. Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar C, Herskin M, Michel V, Miranda Chueca MÁ, Roberts HC, Padalino B, Pasquali P, Spoolder H, Ståhl K, Calvo AV, Viltrop A, Winckler C, Carvelli A, Paillot R, Broglia A, Kohnle L, Baldinelli F, Van der Stede Y. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus-1. EFSA J 2022 Jan;20(1):e07036.
                      doi: 10.2903/j.efsa.2022.7036pubmed: 35035581google scholar: lookup
                    4. Vereecke N, Carnet F, Pronost S, Vanschandevijl K, Theuns S, Nauwynck H. Genome Sequences of Equine Herpesvirus 1 Strains from a European Outbreak of Neurological Disorders Linked to a Horse Gathering in Valencia, Spain, in 2021. Microbiol Resour Announc 2021 May 20;10(20).
                      doi: 10.1128/MRA.00333-21pubmed: 34016681google scholar: lookup
                    5. Kang HW, Lee EY, Lee KK, Ko MK, Park JY, Kim YH, Lee K, Choi EJ, Kim J, So B, Park CK, Jeoung HY. Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea. Pathogens 2021 Apr 2;10(4).
                      doi: 10.3390/pathogens10040425pubmed: 33918404google scholar: lookup
                    6. Oladunni FS, Horohov DW, Chambers TM. EHV-1: A Constant Threat to the Horse Industry. Front Microbiol 2019;10:2668.
                      doi: 10.3389/fmicb.2019.02668pubmed: 31849857google scholar: lookup
                    7. Preziuso S, Sgorbini M, Marmorini P, Cuteri V. Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes. Viruses 2019 Sep 13;11(9).
                      doi: 10.3390/v11090851pubmed: 31540321google scholar: lookup
                    8. Garvey M, Lyons R, Hector RD, Walsh C, Arkins S, Cullinane A. Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017. Pathogens 2019 Jan 15;8(1).
                      doi: 10.3390/pathogens8010007pubmed: 30650561google scholar: lookup
                    9. Matczuk AK, Skarbek M, Jackulak NA, Bażanów BA. Molecular characterisation of equid alphaherpesvirus 1 strains isolated from aborted fetuses in Poland. Virol J 2018 Dec 3;15(1):186.
                      doi: 10.1186/s12985-018-1093-5pubmed: 30509297google scholar: lookup
                    10. Bryant NA, Wilkie GS, Russell CA, Compston L, Grafham D, Clissold L, McLay K, Medcalf L, Newton R, Davison AJ, Elton DM. Genetic diversity of equine herpesvirus 1 isolated from neurological, abortigenic and respiratory disease outbreaks. Transbound Emerg Dis 2018 Jun;65(3):817-832.
                      doi: 10.1111/tbed.12809pubmed: 29423949google scholar: lookup
                    11. Tsujimura K, Murase H, Bannai H, Nemoto M, Yamanaka T, Kondo T. Efficacy of five commercial disinfectants and one anionic surfactant against equine herpesvirus type 1. J Vet Med Sci 2015 Nov;77(11):1545-8.
                      doi: 10.1292/jvms.15-0030pubmed: 26074409google scholar: lookup
                    12. Stasiak K, Rola J, Ploszay G, Socha W, Zmudzinski JF. Detection of the neuropathogenic variant of equine herpesvirus 1 associated with abortions in mares in Poland. BMC Vet Res 2015 May 1;11:102.
                      doi: 10.1186/s12917-015-0416-7pubmed: 25929692google scholar: lookup
                    13. Marenzoni ML, Bietta A, Lepri E, Casagrande Proietti P, Cordioli P, Canelli E, Stefanetti V, Coletti M, Timoney PJ, Passamonti F. Role of equine herpesviruses as co-infecting agents in cases of abortion, placental disease and neonatal foal mortality. Vet Res Commun 2013 Dec;37(4):311-7.
                      doi: 10.1007/s11259-013-9578-6pubmed: 24052369google scholar: lookup
                    14. Bannai H, Kambayashi Y, Kume K, Takebe N, Endo Y, Kawanishi N, Nemoto M, Tsujimura K. Reduction in endemic equine herpesvirus type-1 and type-4 infection among Thoroughbred yearlings through an updated vaccination program. J Equine Sci 2025 Jun;36(2):67-74.
                      doi: 10.1294/jes.36.67pubmed: 40831999google scholar: lookup
                    15. Mohamed E, Zarak I, Vereecke N, Theuns S, Laval K, Nauwynck H. Genomic analysis and replication kinetics of the closely related EHV-1 neuropathogenic 21P40 and abortigenic 97P70 strains. Vet Res 2025 Jan 13;56(1):12.
                      doi: 10.1186/s13567-024-01434-3pubmed: 39806433google scholar: lookup
                    16. Tong P, Pan J, Dang Y, Yang E, Jia C, Duan R, Tian S, Palidan N, Kuang L, Wang C, Lu G, Xie J. First identification and isolation of equine herpesvirus type 1 in aborted fetal lung tissues of donkeys. Virol J 2024 May 27;21(1):117.
                      doi: 10.1186/s12985-024-02390-2pubmed: 38802935google scholar: lookup
                    17. Emelogu U, Lewin AC, Balasuriya UBR, Liu CC, Wilkes RP, Zhang J, Mills EP, Carter RT. Phylogenomic assessment of 23 equid alphaherpesvirus 1 isolates obtained from USA-based equids. Virol J 2023 Nov 29;20(1):278.
                      doi: 10.1186/s12985-023-02248-zpubmed: 38031153google scholar: lookup
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