Probiotic potential of Weissella strains isolated from horse feces.
Abstract: In this study, we isolated four Weissella confusa strains from the healthy horse feces to test their potential as equine probiotics. The identification and characteristics of these isolates were determined as per standard methods. Resistance and susceptibility of the isolated strains were tested to low pHs, different heat treatments, commonly used antibiotics and against the pathogenic strains of Salmonella, Pasteurella, Staphylococcus aureus, and Escherichia coli. After 3 h cultural in different pH medium, the 4 strains still had a certain amount of survival above pH 3.0. WH2 and WH4 were still viable at pH2.5. All the isolated strains showed proper growth at 60 °C while no strain survived at 80 °C. The inhibition of α-amylase, the scavenging ability of free radical DPPH· and hydroxyl free radical HO·were also investigated. The results showed that WH4 had highest inhibition rate of α-amylase activity and DPPH· free radical scavenging rate, and the inhibition rate of α-amylase activity was 24.09% and the DPPH· free radical scavenging rate was 35.78%. The inhibition rate ofα-amylase activity and DPPH· scavenging rate of free radicals in the other three strains were about 10%. The clearance rate of hydroxyl radical (HO·) in 4 strains was between 12% and 15%. The antibiotic susceptibilities varied for these four Weisella strains but all of them showed resistance against the frequently used equine antibiotics. All the four strains successfully suppressed the growth of standard strains in in vitro bacteriostasis experiment, which included Salmonella enteritidis (NTNC13349), Escherichia coli (C83902) and Staphylococcus aureus (BNCC186335). they also successfully suppressed the growth of state key laboratory isolating pathogens, which are Pasterurella multocida and Salmonella. Our findings suggest that the isolated strains of Weissella confusa can act as potential equine probiotics and should be explored further.
Copyright © 2019 Elsevier Ltd. All rights reserved.
Publication Date: 2019-04-19 PubMed ID: 31009656DOI: 10.1016/j.micpath.2019.04.032Google Scholar: Lookup
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Summary
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This research investigates the viability of Weissella confusa strains, isolated from healthy horse feces, to act as probiotics for horses. The strains were assessed based on their survival under varying conditions, their impact on pathogenic strains, and antibiotic resistance.
Isolation and Identification of Weissella Confusa Strains
- Researchers isolated four strains of Weissella confusa from healthy horse feces.
- Conventional methods were applied to determine the identification and characteristics of these strains.
Testing Resistance and Susceptibility
- The isolated strains were tested for their resistance against varying pH levels, different heat treatments, and commonly used antibiotics.
- Even after three hours in varying pH medium environments, all four strains still demonstrated survival above pH 3.0. Upon further testing, two specific strains, WH2 and WH4, remained viable at pH 2.5.
- When subjected to heat treatments, all four strains presented proper growth at 60°C while none survived at 80°C.
- The antibiotic susceptibilities differed among these isolates but all exhibited resistance against the frequently used equine antibiotics.
Influence on Pathogenic Strains
- The probiotic strains were tested against pathogenic bacteria such as Salmonella, Pasteurella, Staphylococcus aureus, and Escherichia coli.
- All four strains succeeded in suppressing the growth of the standard pathogens in in-vitro bacteriostasis tests, including strains like Salmonella enteritidis, Escherichia coli, Staphylococcus aureus, Pasterurella multocida, and Salmonella.
Additional Experiments and Characteristics
- Further research explored the strains’ inhibition of α-amylase, a digestive enzyme, and their capacity to scavenge DPPH· and hydroxyl free radical HO·.
- The strain WH4 demonstrated the highest inhibition rate of α-amylase activity and DPPH· free radical scavenging rate.
- The other three strains had approximately 10% inhibition rate of α-amylase activity and DPPH· scavenging rate of free radicals.
- The removal rate of the hydroxyl radical (HO·) among the strains was between 12% and 15%.
Conclusion
- Based on these results, the isolated strains of Weissella confusa demonstrate potential for use as equine probiotics. However, the authors suggest that further exploration is necessary.
Cite This Article
APA
Xia Y, Qin S, Shen Y.
(2019).
Probiotic potential of Weissella strains isolated from horse feces.
Microb Pathog, 132, 117-123.
https://doi.org/10.1016/j.micpath.2019.04.032 Publication
Researcher Affiliations
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
- College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. Electronic address: yshen@mail.hzau.edu.cn.
MeSH Terms
- Animals
- Anti-Bacterial Agents / pharmacology
- Biphenyl Compounds
- Escherichia coli / growth & development
- Feces / microbiology
- Horses
- Hydrogen-Ion Concentration
- Hydroxyl Radical
- Microbial Sensitivity Tests
- Microbial Viability / drug effects
- Pasteurella / growth & development
- Phylogeny
- Picrates
- Probiotics / isolation & purification
- Salmonella / growth & development
- Sequence Analysis, DNA
- Staphylococcus aureus / growth & development
- Stress, Physiological
- Weissella / drug effects
- Weissella / growth & development
- Weissella / isolation & purification
- Weissella / physiology
- alpha-Amylases / metabolism
Citations
This article has been cited 14 times.- Yang B, Song H, Hu R, Tao L, Liang Z, Cong W, Kang Y. Weissella confusa N17 Derived from Loach (Misgurnus anguillicaudatus) Exhibits Promising for Further Applications in Loach Aquaculture.. Probiotics Antimicrob Proteins 2023 Aug 26;.
- Wang X, Zhang D, Dai Z, Yu J, Li Q, Zhang L. A Case of Weissella confusa Isolated from Blood of a Patient with Coronary Heart Disease Complicated with Gastrointestinal Bleeding.. Infect Drug Resist 2023;16:4571-4577.
- Lei J, Ran X, Guo M, Liu J, Yang F, Chen D. Screening, Identification, and Probiotic Properties of Bacillus Pumilus From Yak.. Probiotics Antimicrob Proteins 2023 Mar 30;.
- Bao W, Yu J, He Y, Liu M, Yang X. The diversity analysis and gene function prediction of intestinal bacteria in three equine species.. Front Microbiol 2022;13:973828.
- Wu C, Dai C, Tong L, Lv H, Zhou X. Evaluation of the Probiotic Potential of Lactobacillus delbrueckii ssp. indicus WDS-7 Isolated from Chinese Traditional Fermented Buffalo Milk In Vitro.. Pol J Microbiol 2022 Mar 30;71(1):91-105.
- Zeng Z, Zhang J, Li Y, Li K, Gong S, Li F, Wang P, Iqbal M, Kulyar MF, Li J. Probiotic Potential of Bacillus licheniformis and Bacillus pumilus Isolated from Tibetan Yaks, China.. Probiotics Antimicrob Proteins 2022 Jun;14(3):579-594.
- Zhang X, Zhang H, Jiao P, Xia M, Tang B. Preparation and Evaluation of Antioxidant Activities of Bioactive Peptides Obtained from Cornus officinalis.. Molecules 2022 Feb 11;27(4).
- Goodman-Davis R, Figurska M, Cywinska A. Gut Microbiota Manipulation in Foals-Naturopathic Diarrhea Management, or Unsubstantiated Folly?. Pathogens 2021 Sep 4;10(9).
- Zeng Z, He X, Li F, Zhang Y, Huang Z, Wang Y, Li K, Bao Y, Iqbal M, Fakhar-E-Alam Kulyar M, Li J. Probiotic Properties of Bacillus proteolyticus Isolated From Tibetan Yaks, China.. Front Microbiol 2021;12:649207.
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