Procalcitonin Detection in Veterinary Species: Investigation of Commercial ELISA Kits.
Abstract: In human medicine, procalcitonin (PCT), the precursor of calcitonin, is used for the rapid identification of the origin and severity of sepsis. In veterinary medicine, PCT has been studied in horses, cattle, and dogs, but the use of PCT in diagnostic and/or prognostic settings is not possible because of the lack of validated assays to obtain reference ranges. The aim of the present study was the investigation of commercially available ELISA kits for the detection of canine and equine PCT in plasma samples. Validation of the ELISA kits was performed by using species-specific recombinant proteins spiked both in plasma and buffer samples; linearity, limit of detection (LOD), recovery, and intra-assay and inter-assay variability were calculated. Moreover, clinical samples obtained from sick and healthy animals were also analyzed with the tested kits. Canine PCT was measured with a recombinant canine and a canine PCT ELISA kit. Equine PCT was measured with an equine and a human ELISA PCT kit. Our data demonstrate that the canine recombinant PCT ELISA kit can be used to measure canine PCT in plasma samples, showing an intra-assay and inter-assay coefficient of variation less than 20% and a LOD of 11 pg/mL, whereas the present results do not support the use of the canine PCT ELISA kit. The human PCT ELISA kit is suitable to detect equine PCT with a LOD of 56 ng/mL, whereas the equine PCT ELISA kit did not detect recombinant equine PCT.
Publication Date: 2020-08-26 PubMed ID: 32859059PubMed Central: PMC7552142DOI: 10.3390/ani10091511Google Scholar: Lookup
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- Journal Article
- Biomarkers
- Clinical Pathology
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Diseases
- Equine Health
- Horses
- Immune Response
- In Vitro Research
- Inflammatory Response
- Laboratory Methods
- Plasma
- Prognosis
- Sepsis
- Species Comparison
- Veterinary Medicine
- Veterinary Research
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the effectiveness of commercially available ELISA kits for detecting procalcitonin (PCT) in canines and equines. The results highlight that the recombinant canine PCT ELISA kit is effective for PCT detection in canines, while the ELISA kit for equine PCT is not ideal, with the human PCT ELISA kit displaying better accuracy.
Objective of the Study
- The goal of this study was to assess the adequacy of commercial ELISA kits for identifying procalcitonin (PCT) in canine and equine plasma samples. PCT, a precursor to calcitonin, is a valuable marker in human medicine for rapidly determining the origin and severity of sepsis. However, in veterinary medicine, using PCT for diagnostic or prognostic purposes has been challenged by a lack of validated assays to establish reference ranges.
Methods Employed in the Study
- Validation of the ELISA kits was accomplished by spiking species-specific recombinant proteins into both plasma and buffer samples. Linearity, limit of detection (LOD), recovery rate, and intra-assay and inter-assay variability were then calculated.
- The research also tested clinical samples procured from both healthy and infected animals using the evaluated kits.
- Detection involved the use of a recombinant canine and a canine PCT ELISA kit for canine PCT, and an equine and a human ELISA PCT kit for equine PCT.
Key Findings
- The research demonstrated that the canine recombinant PCT ELISA kit could effectively be used to detect canine PCT in plasma samples, with an intra-assay and inter-assay coefficient of variation below 20%, and an LOD of 11 pg/mL. On the other hand, the results do not endorse the use of the standard canine PCT ELISA kit.
- For equine PCT, the human PCT ELISA kit proved to be more effective than the equine PCT ELISA kit, with a LOD of 56 ng/mL. The equine PCT ELISA kit failed to detect recombinant equine PCT.
Implications of the Study
- The findings from this study provide significant insights into the use of ELISA kits for PCT detection in veterinary medicine.
- The study underscores the need for improved diagnostic tools in veterinary medicine, especially in the context of PCT detection where the currently available tools prove to be inconsistent or underperforming.
Cite This Article
APA
Battaglia F, Meucci V, Tognetti R, Bonelli F, Sgorbini M, Lubas G, Pretti C, Intorre L.
(2020).
Procalcitonin Detection in Veterinary Species: Investigation of Commercial ELISA Kits.
Animals (Basel), 10(9), 1511.
https://doi.org/10.3390/ani10091511 Publication
Researcher Affiliations
- Department of Veterinary Science, University of Pisa, 56122 Pisa, Italy.
- Department of Veterinary Science, University of Pisa, 56122 Pisa, Italy.
- Department of Veterinary Science, University of Pisa, 56122 Pisa, Italy.
- Department of Veterinary Science, University of Pisa, 56122 Pisa, Italy.
- Department of Veterinary Science, University of Pisa, 56122 Pisa, Italy.
- Department of Veterinary Science, University of Pisa, 56122 Pisa, Italy.
- Department of Veterinary Science, University of Pisa, 56122 Pisa, Italy.
- Department of Veterinary Science, University of Pisa, 56122 Pisa, Italy.
Grant Funding
- project PRA_2018/2019_54. / University of Pisa, Italy,
Conflict of Interest Statement
The authors declare no conflict of interest.
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Citations
This article has been cited 1 times.- Neumann S, Siegert S, Fischer A. Procalcitonin as an Endogenous Biomarker for Mastitis in Cows.. Animals (Basel) 2023 Jul 5;13(13).
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