Protective effect against Rhodococcus equi infection in mice of IgG purified from horses vaccinated with virulence associated protein (VapA)-enriched antigens.
Abstract: IgG was purified from horses immunized with repeated doses of virulence associated (VapA) enriched antigens extracted with Triton X-114 from the surface of a virulent strain of R. equi. This IgG were administered to mice immunosuppressed by prior treatment with indomethacin. Mice administered the higher dose were completely protected against intraperitoneal infection with R. equi; mice given the lower dose were partially protected. By contrast, mice administered concentrated nonimmune equine IgG were not protected. This study demonstrates that VapA may be an important antigen involved in humoral protective immunity in R. equi infections caused by foal virulent strains.
Publication Date: 1997-06-16 PubMed ID: 9226833DOI: 10.1016/s0378-1135(97)00087-4Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates the protective effect against Rhodococcus equi infection in mice when administered IgG purified from horses that have been vaccinated with virulence-associated protein (VapA)-enriched antigens. The study demonstrated that these antigens extracted from a virulent strain of R. equi provided significant protection against the infection.
Research Methodology
- The first step in the study was to immunize horses with repeated doses of virulence-associated protein (VapA) enriched antigens. These antigens were extracted with a detergent called Triton X-114 from the surface of a highly virulent strain of R. equi.
- The next step was to purify Immunoglobulin G (IgG) from these immunized horses. IgG is a type of antibody that plays a crucial role in immune response to bacterial and viral infections.
- The purified IgG was then administered to mice which had their immune response suppressed through prior treatment with a drug known as indomethacin. This created a more challenging environment for the protective antigens to function, ensuring robustness of the study’s findings.
Findings
- The study found that the mice which were administered a higher dose of the purified IgG were completely protected from an intraperitoneal infection with R. equi, a type of infection involving the membranes of the abdominal wall.
- Mice given a lower dose of the purified IgG were partially protected.
- In contrast, mice administered non-immune equine IgG, which did not contain the specific VapA-enriched antigens, were not protected against the infection.
Implications of the Study
- The research concludes that VapA is a critical antigen in the fight against R. equi infections. In other words, the body generates an effective immune response against R. equi when it recognizes this particular protein.
- This study suggests potential applications for the prevention and treatment of R. equi infections in mammals, including horses and presumably humans, through the use of vaccines enriched with VapA antigens.
Cite This Article
APA
Fernandez AS, Prescott JF, Nicholson VM.
(1997).
Protective effect against Rhodococcus equi infection in mice of IgG purified from horses vaccinated with virulence associated protein (VapA)-enriched antigens.
Vet Microbiol, 56(3-4), 187-192.
https://doi.org/10.1016/s0378-1135(97)00087-4 Publication
Researcher Affiliations
- Buenos Aires Province Central University, Tandil, Argentina.
MeSH Terms
- Actinomycetales Infections / immunology
- Actinomycetales Infections / prevention & control
- Actinomycetales Infections / veterinary
- Animals
- Antibody Formation
- Antigens, Bacterial / immunology
- Bacterial Proteins / immunology
- Bacterial Vaccines
- Detergents
- Enzyme-Linked Immunosorbent Assay
- Horse Diseases
- Horses
- Immunization, Passive
- Immunoglobulin G / blood
- Immunoglobulin G / isolation & purification
- Immunoglobulin G / therapeutic use
- Immunosuppression Therapy
- Indomethacin / pharmacology
- Lipoproteins / immunology
- Mice
- Octoxynol
- Polyethylene Glycols
- Rhodococcus equi / immunology
- Virulence Factors
Citations
This article has been cited 4 times.- Lohmann KL, Lopez AM, Manning ST, Marques FJ, Brownlie R, Allen AL, Sangster AE, Mutwiri G, Gerdts V, Potter A, Townsend HG. Failure of a VapA/CpG oligodeoxynucleotide vaccine to protect foals against experimental Rhocococcus equi pneumonia despite induction of VapA-specific antibody and interferon-γ response.. Can J Vet Res 2013 Jul;77(3):161-9.
- Hooper-McGrevy KE, Wilkie BN, Prescott JF. Immunoglobulin G subisotype responses of pneumonic and healthy, exposed foals and adult horses to Rhodococcus equi virulence-associated proteins.. Clin Diagn Lab Immunol 2003 May;10(3):345-51.
- Lopez AM, Hines MT, Palmer GH, Alperin DC, Hines SA. Identification of pulmonary T-lymphocyte and serum antibody isotype responses associated with protection against Rhodococcus equi.. Clin Diagn Lab Immunol 2002 Nov;9(6):1270-6.
- Giguère S, Hondalus MK, Yager JA, Darrah P, Mosser DM, Prescott JF. Role of the 85-kilobase plasmid and plasmid-encoded virulence-associated protein A in intracellular survival and virulence of Rhodococcus equi.. Infect Immun 1999 Jul;67(7):3548-57.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
