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Home / Equine Research Bank / Parasit Vectors / Protein expression profile of Gasterophilus intestinalis lar...
Parasites & vectors2009; 2(1); 6; doi: 10.1186/1756-3305-2-6

Protein expression profile of Gasterophilus intestinalis larvae causing horse gastric myiasis and characterization of horse immune reaction.

Abstract: Little information is available on the immunological aspect of parasitic Gasterophilus intestinalis (Diptera, Oestridae) larvae causing horse gastric myiasis. The objectives of this research were to analyze the protein content of larval crude extracts of the migrating second and third larvae (L2 and L3) of G. intestinalis in order to characterize the immune response of horses. Results: The proteomic profile of L2 and L3, investigated by using one and two dimensional approaches, revealed a migration pattern specific to each larval stage. Furthermore, Western blots were performed with horse sera and with sera of Balb/c mice immunised with the larval crude extracts of L2 or L3, revealing a different immune reaction in naturally infected horses vs. artificially induced immune reaction in mice. The comparisons of the immunoblot profiles demonstrate that the stage L2 is more immunogenic than the stage L3 most likely as an effect of the highest enzymatic production of L2 while migrating through the host tissues. Fifteen proteins were identified by mass spectrometry. Conclusions: This work provides further information into the understanding of the interaction between G. intestinalis and their host and by contributing a novel scheme of the proteomic profile of the main larval stages.
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Publication Date: 2009-01-08 PubMed ID: 19133133PubMed Central: PMC2633288DOI: 10.1186/1756-3305-2-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the immune reaction horses have to parasitic larvae Gasterophilis intestinalis, a cause of gastric myiasis, by analyzing the protein content these larvae produce during their secondary and tertiary stages of growth. The study findings indicate that the second stage of larvae development is more immune-reactive than the third stage, possibly due to a higher level of enzyme production during their migration through horse tissues.

Study Objective and Methodology

  • The central aim of the study was to explore the immunological aspect of horse gastric myiasis, a condition caused by Gasterophilis intestinalis larvae, by examining the protein content of the larvae during their second (L2) and third (L3) stages of maturation.
  • Utilizing one and two-dimensional methods, the researchers analyzed the proteomic (protein) profile of L2 and L3 larval crude extracts.

Results and Analysis

  • Each larval stage exhibited a unique migration pattern.
  • By performing Western blots with horse and Balb/c mice sera (mice immunised artificially with L2 or L3 larval extracts), the researchers observed a disparity in immune responses between naturally infected horses and artificially induced reactions in mice.
  • Specifically, the L2 stage was found to be more immunogenic (capable of producing an immune response) than the L3 stage, possibly due to increased enzymatic production in L2 larvae during their migration through host tissues.
  • Fifteen proteins produced by the larvae were successfully identified via a technique called mass spectrometry.

Conclusions

  • This study provides new understanding of the interaction between the G. intestinalis larvae and their host – horses.
  • Furthermore, the study provides a fresh perspective of the proteomic profile of these larvae during their principal development stages, which may prove useful in future research and interventions regarding gastric myiasis in horses.

Cite This Article

APA
Roelfstra L, Deeg CA, Hauck SM, Buse C, Membrez M, Betschart B, Pfister K. (2009). Protein expression profile of Gasterophilus intestinalis larvae causing horse gastric myiasis and characterization of horse immune reaction. Parasit Vectors, 2(1), 6. https://doi.org/10.1186/1756-3305-2-6

Publication

Parasites & vectors
ISSN: 1756-3305
NlmUniqueID: 101462774
Country: England
Language: English
Volume: 2
Issue: 1
Pages: 6

Researcher Affiliations

Roelfstra, Liselore
  • Institute of Biology, University of Neuchâtel, Switzerland. liselore.roelfstra@unine.ch.
Deeg, Cornelia A
    Hauck, Stefanie M
      Buse, Christina
        Membrez, Mathieu
          Betschart, Bruno
            Pfister, Kurt

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              Citations

              This article has been cited 3 times.
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