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Scientific reports2018; 8(1); 5249; doi: 10.1038/s41598-018-23537-6

Proteins involved in embryo-maternal interaction around the signalling of maternal recognition of pregnancy in the horse.

Abstract: During maternal recognition of pregnancy (MRP), a conceptus-derived signal leads to the persistence of the corpus luteum and the maintenance of gestation. In the horse, the nature of this signal remains to be elucidated. Several studies have focused on the changes in gene expression during MRP, but little information exists at the protein level. The aim of this study was to identify the proteins at the embryo-maternal interface around signalling of MRP in the horse (day 13) by means of mass spectrometry. A distinct influence of pregnancy was established, with 119 proteins differentially expressed in the uterine fluid of pregnant mares compared to cyclic mares and with upregulation of several inhibitors of the prostaglandin synthesis during pregnancy. By creating an overview of the proteins at the embryo-maternal interface in the horse, this study provides a solid foundation for further targeted studies of proteins potentially involved in embryo-maternal interactions, MRP and pregnancy loss in the horse.
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Publication Date: 2018-03-27 PubMed ID: 29588480PubMed Central: PMC5869742DOI: 10.1038/s41598-018-23537-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers conducted a study on horses to identify proteins involved in the embryo-maternal interaction, specifically during the maternal recognition of pregnancy (MRP). Using mass spectrometry, they found that pregnancy influenced the expression of 119 proteins in the uterine fluid of pregnant mares, and several inhibitors of prostaglandin synthesis were upregulated during pregnancy.

Objective of Research

  • The primary objective of this study was to identify and examine the proteins involved in the interaction between an embryo and its mother around the signalling of maternal recognition of pregnancy (MRP) in horses. Identifying these proteins would provide significant insights into the mechanisms of MRP and offer a foundation for further research into pregnancy loss in horses.

Methodology

  • To identify these proteins, the research team employed a method called mass spectrometry. They chose this technique as it is capable of identifying and quantifying individual proteins in a mixture based on their distinctive mass.
  • The study focused on day 13 of the MRP, a critical period when the conceptus-derived signal leads to the persistence of the corpus luteum, which is necessary for the maintenance of gestation in horses.
  • This research compared the proteins found in the uterine fluid of pregnant mares and cyclic mares to identify any differences.

Findings

  • The study discovered a significant influence of pregnancy on protein expression, with 119 proteins differentially expressed in the uterine fluid of pregnant mares compared to cyclic mares. This finding indicates that there are specific proteins associated with pregnancy and potentially with the process of maternal recognition of pregnancy.
  • Furthermore, the research observed an upregulation of various inhibitors of prostaglandin synthesis during pregnancy. As prostaglandins play a crucial role in various reproductive processes, including the regulation of the menstrual cycle and the induction of labor, an increased presence of these inhibitors during pregnancy could have significant implications.

Implications

  • The results provide a comprehensive overview of the proteins present at the embryo-maternal interface in horses, particularly during the signal of MRP. This knowledge could form the basis for further targeted studies into these proteins and their potential role in embryo-maternal interactions and pregnancy loss in horses.
  • The study’s discoveries surrounding the upregulation of inhibitors of prostaglandin synthesis also open up new avenues for research into the regulation of prostaglandin during pregnancy.

Cite This Article

APA
Smits K, Willems S, Van Steendam K, Van De Velde M, De Lange V, Ververs C, Roels K, Govaere J, Van Nieuwerburgh F, Peelman L, Deforce D, Van Soom A. (2018). Proteins involved in embryo-maternal interaction around the signalling of maternal recognition of pregnancy in the horse. Sci Rep, 8(1), 5249. https://doi.org/10.1038/s41598-018-23537-6

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 8
Issue: 1
Pages: 5249

Researcher Affiliations

Smits, Katrien
  • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Katrien.Smits@UGent.be.
Willems, Sander
  • Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Gent, Belgium.
Van Steendam, Katleen
  • Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Gent, Belgium.
Van De Velde, Margot
  • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
De Lange, Valérie
  • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Ververs, Cyrillus
  • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Roels, Kim
  • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Govaere, Jan
  • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Van Nieuwerburgh, Filip
  • Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Gent, Belgium.
Peelman, Luc
  • Laboratory of Animal Genetics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Deforce, Dieter
  • Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Gent, Belgium.
Van Soom, Ann
  • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

MeSH Terms

  • Animals
  • Embryo, Mammalian / embryology
  • Embryo, Mammalian / metabolism
  • Female
  • Horses / embryology
  • Horses / metabolism
  • Pregnancy
  • Protein Interaction Maps
  • Proteins / metabolism
  • Signal Transduction
  • Uterus / metabolism

Conflict of Interest Statement

The authors declare no competing interests.

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