Proteomic Differences Between the Ovulatory and Anovulatory Sides of the Mare’s Follicular and Oviduct Fluid.
Abstract: The follicular fluid and oviduct fluid play major roles in oocyte maturation, sperm activation, and fertilization. To better understand the physiological environments for equine oocyte maturation and fertilization, here we conducted the proteome analysis and comparison on follicular fluids and oviduct fluids from the ovulatory side and the anovulatory side. The results showed that there is no significant difference between two side oviduct fluids, but a total of 71 differential abundance proteins (DAPs) were identified between two side follicular fluids, of which 9 are up-regulated and 62 are down-regulated in ovulatory side follicle fluid versus anovulatory side follicle fluid. As we expected, the function classification and enrichment results indicate that up- and down-regulated proteins are largely related to oocyte meiosis, maturation and ovulation. Noticeably, among 9 up-regulated DAPs in ovulatory side follicle fluid, as the DAP with the greatest fold change, PLA2G1B may be a newly discovered component that influences the efficacy of horse IVM/IVF. The current findings add to our knowledge of the in vivo conditions and regulation of equine reproduction, as well as the regulatory mechanism underpinning alternative ovulation.
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
Publication Date: 2022-12-30 PubMed ID: 36592664DOI: 10.1016/j.jevs.2022.104207Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research compares the protein concentrations in fluid gathered from the ovulatory and anovulatory sides of mare reproductive systems. The findings suggest differences in protein levels may affect oocyte maturation and fertility.
Objectives and Methodology of the Research
- This study aimed to comprehend the physiological environments that influence equine oocyte maturation and fertilization.
- The researchers conducted a proteome analysis (study of the entire set of proteins) on fluids from the follicular structure and oviduct collected from both the ovulatory (when an egg is released) and anovulatory (when an egg is not released) sides.
Key Findings
- No significant differences were found when comparing the fluid from the ovulatory and anovulatory sides of the oviduct. This suggests that the physiological environment of the oviduct is consistent irrespective of whether an egg is being released or not.
- However, differences were noticed when comparing the follicular fluids from the ovulatory and anovulatory sides: 71 proteins with differential abundance (DAPs) were identified. Out of these, 9 were found in higher levels (up-regulated) and 62 were found in lower levels (down-regulated) in the ovulatory follicular fluid compared to the anovulatory fluid.
- These differentially abundant proteins were associated largely with oocyte meiosis (the cell division process that produces eggs), maturation, and ovulation – suggesting that these proteins may play a key role in these processes.
Significance and Implications
- The protein PLA2G1B, which was elevated in the ovulatory side follicle fluid, could be a novel factor affecting the efficacy of horse In Vitro Maturation (IVM) and In Vitro Fertilization (IVF).
- The researchers argue that an understanding of these protein differences could provide insights into the physiological conditions impacting equine reproduction and the mechanism behind alternate ovulation.
- This study could potentially lead to improved fertility treatments and reproductive strategies in equine veterinary practice, though more research is needed to confirm and further understand these potential impacts.
Cite This Article
APA
Shen Y, Ulaangerel T, Ren H, Davshilt T, Yi M, Li X, Xing J, Du M, Bai D, Dugarjav M, Bou G.
(2022).
Proteomic Differences Between the Ovulatory and Anovulatory Sides of the Mare’s Follicular and Oviduct Fluid.
J Equine Vet Sci, 121, 104207.
https://doi.org/10.1016/j.jevs.2022.104207 Publication
Researcher Affiliations
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: dmanglai@163.com.
- College of Animal Science, Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Inner Mongolia Agricultural University, Hohhot, China. Electronic address: gerelchimeg@imau.edu.cn.
MeSH Terms
- Animals
- Horses
- Female
- Male
- Ovarian Follicle / metabolism
- Proteomics
- Semen
- Oocytes / metabolism
- Anovulation / veterinary
- Oviducts
- Horse Diseases / metabolism
Citations
This article has been cited 1 times.- Song H, Dong T, Wang W, Yan X, Geng C, Bai S, Hu H. GWAS Enhances Genomic Prediction Accuracy of Caviar Yield, Caviar Color and Body Weight Traits in Sturgeons Using Whole-Genome Sequencing Data. Int J Mol Sci 2024 Sep 9;25(17).
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